Biofilm Production Potential of  Serovars Isolated from Chickens in North West Province, South Africa

STEPHEN ABIOLA AKINOLA; MPINDA EDOAURD TSHIMPAMBA; MULUNDA MWANZA; COLLINS NJIE ATEBA

Accès libre

Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa

STEPHEN ABIOLA AKINOLA

MPINDA EDOAURD TSHIMPAMBA

MULUNDA MWANZA

COLLINS NJIE ATEBA

| 23 nov. 2020

Polish Journal of Microbiology

Édition 69 (2020): Edition 4 (December 2020)

À propos de cet article

Article précédent

Article suivant

Citez

Partagez

Article Category: original-paper

Publié en ligne: 23 nov. 2020

Pages: 427 - 439

Reçu: 10 août 2020

Accepté: 24 sept. 2020

DOI: https://doi.org/10.33073/pjm-2020-046

Mots clés
biofilm, biofilm production potential, crystal violet microtitre

© 2020 Stephen Abiola Akinola et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effect of incubation temperatures on biofilm-forming potentials of Salmonella serotypes.

Behavioral pattern of Salmonella serotypes to biofilm production at 25°C incubation temperature.

Behavioral pattern of Salmonella serotypes to biofilm production at 37°C incubation temperature.

Behavioral pattern of Salmonella serotypes to biofilm production at 40°C incubation temperature.

Identities of Salmonella isolates used for biofilm assay.

Isolate number	Sources	Accession number	Organism
CHG1	Broiler	MG663456	Salmonella enterica subsp. enterica
CHG2	Broiler	MG663457	Salmonella enterica subsp. enterica
CHG3	Broiler	MG663458	Salmonella enterica subsp. enterica
CHG4	Broiler	MG663459	Salmonella enterica ser. Weltevreden
CHG5	Broiler	MG663460	Salmonella enterica ser. Chingola
CHG6	Broiler	MG663461	Salmonella enterica ser. Arizonae
CHG7	Broiler	MG663462	Salmonella enterica ser. Bovismorbificans
CHG8	Layer	MG663463	Salmonella enterica subsp. enterica
CHG9	Layer	MG663464	Salmonella enterica subsp. enterica
CHG10	Layer	MG663465	Salmonella enterica ser. Typhimurium
CHG11	Layer	MG663466	Salmonella enterica ser. Salamae
CHG12	Layer	MG663467	Salmonella enterica ser. Houten
CHG13	Layer	MG663468	Salmonella enterica subsp. enterica
CHG14	Indigenous Venda	MG663469	Salmonella enterica ser. Bareilly
CHG15	Indigenous Venda	MG663470	Salmonella enterica subsp. enterica
CHG16	Indigenous Venda	MG663471	Salmonella enterica subsp. enterica
CHG17	Indigenous Venda	MG663472	Salmonella enterica subsp. enterica
CHG18	Indigenous Venda	MG663473	Salmonella enterica ser. Heidelberg
CHG19	Indigenous Venda	MG663474	Salmonella enterica ser. Arizonae
CHG20	Indigenous Venda	MG663475	Salmonella enterica subsp. enterica
CHG21	Indigenous Venda	MG663476	Salmonella enterica ser. India
CHG22	Indigenous Venda	MG663477	Salmonella enterica ser. Crossness
CHG23	Indigenous Venda	MG663478	Salmonella enterica ser. Albany
CHG24	Indigenous Venda	MG663479	Salmonella enterica ser. Yovokome
CHG25	Indigenous Venda	MG663480	Salmonella enterica ser. Pullorum
CHG26	Indigenous Venda	MG663481	Salmonella enterica ser. Infantis
CHG27	Broiler	MG663482	Salmonella enterica ser. Arizonae
CHG28	Broiler	MG663483	Salmonella enterica ser. Heidelberg
CHG29	Broiler	MG663484	Salmonella enterica subsp. enterica
CHG30	Broiler	MG663485	Salmonella enterica subsp. enterica
CHG31	Broiler	MG663486	Salmonella bongori
CHG32	Broiler	MG663487	Salmonella bongori
CHG33	Broiler	MG663488	Salmonella enterica ser. Arizonae
CHG34	Layer	MG663489	Salmonella enterica subsp. enterica
CHG35	Layer	MG663490	Salmonella enterica ser. Wandsworth
CHG36	Layer	MG663491	Salmonella enterica subsp. enterica
CHG37	Layer	MG663492	Salmonella bongori
CHG38	Layer	MG663493	Salmonella enterica ser. Kentucky
CHG39	Layer	MG663494	Salmonella bongori
CHG40	Layer	MG663495	Salmonella enterica ser. Blockley
CHG41	Layer	MG663496	Salmonella enterica ser. Newport
CHG42	Layer	MG663497	Salmonella enterica ser. Typhimurium
CHG43	Indigenous koekoek	MG663498	Salmonella bongori
CHG44	Indigenous koekoek	MG663499	Salmonella enterica ser. Manchester
CHG45	Indigenous koekoek	MG663500	Salmonella enterica subsp. enterica
CHG46	Indigenous koekoek	MG663501	Salmonella enterica subsp. enterica
CHG47	Indigenous koekoek	MG663502	Salmonella enterica ser. Typhimurium
CHG48	Indigenous koekoek	MG663503	Salmonella enterica subsp. enterica
CHG49	Indigenous koekoek	MG663504	Salmonella enterica ser. Typhimurium
CHG50	Indigenous koekoek	MG663505	Salmonella enterica ser. Typhimurium
CHG51	Indigenous koekoek	MG663506	Salmonella enterica ser. Typhimurium
CHG52	Indigenous koekoek	MG663507	Salmonella enterica ser. Koessen
CHG53	Indigenous koekoek	MG663508	Salmonella bongori
CHG54	Indigenous koekoek	MG663509	Salmonella enterica ser. Blegdam
CHG55	Indigenous koekoek	MG663456	Salmonella enterica subsp. enterica

Optical densities and degree of biofilms formed by Salmonella serotypes as influenced by incubation temperatures.

ID	Salmonella isolates	Incubation temperature			Degree of biofilms formed
ID	Salmonella isolates	25°C	37°C	40°C	25°C	37°C	40°C
CHG1	Salmonella enterica subsp. enterica	0.107 ± 0.003	0.312 ± 0.089	0.132 ± 0.020	Moderate	Weak	Moderate
CHG2	Salmonella enterica subsp. enterica	0.075 ± 0.009	0.969 ± 0.065	0.342 ± 0.106	Moderate	Strong	Strong
CHG3	Salmonella enterica subsp. enterica	0.023 ± 0.018	0.946 ± 0.123	0.063 ± 0.032	No biofilm	Strong	Weak
CHG8	Salmonella enterica subsp. enterica	0.247 ± 0.099	0.271 ± 0.030	0.300 ± 0.071	Strong	Weak	Strong
CHG9	Salmonella enterica subsp. enterica	0.120 ± 0.052	0.291 ± 0.015	0.082 ± 0.041	Moderate	Weak	Weak
CHG13	Salmonella enterica subsp. enterica	0.095 ± 0.017	0.319 ± 0.058	0.261 ± 0.081	Moderate	Weak	Strong
CHG15	Salmonella enterica subsp. enterica	0.037 ± 0.013	1.006 ± 0.031	0.167 ± 0.136	Weak	Strong	Moderate
CHG16	Salmonella enterica subsp. enterica	0.067 ± 0.009	1.022 ± 0.108	0.085 ± 0.033	Moderate	Strong	Weak
CHG17	Salmonella enterica subsp. enterica	0.405 ± 0.222	1.010 ± 0.045	0.082 ± 0.060	Strong	Strong	Weak
CHG20	Salmonella enterica subsp. enterica	0.278 ± 0.071	0.885 ± 0.120	0.083 ± 0.027	Strong	Strong	Weak
CHG29	Salmonella enterica subsp. enterica	0.149 ± 0.061	0.961 ± 0.180	0.077 ± 0.024	Strong	Moderate	Weak
CHG30	Salmonella enterica subsp. enterica	0.303 ± 0.085	0.591 ± 0.174	0.112 ± 0.006	Strong	Strong	Moderate
CHG34	Salmonella enterica subsp. enterica	0.039 ± 0.032	1.227 ± 0.273	0.010 ± 0.005	Weak	Weak	No biofilm
CHG36	Salmonella enterica subsp. enterica	0.026 ± 0.024	0.704 ± 0.220	0.065 ± 0.046	No biofilm	Weak	Weak
CHG45	Salmonella enterica subsp. enterica	0.800 ± 0.572	0.983 ± 0.177	1.098 ± 0.736	Strong	Weak	Strong
CHG46	Salmonella enterica subsp. enterica	0.937 ± 0.668	1.017 ± 0.244	1.089 ± 0.803	Strong	Weak	Strong
CHG48	Salmonella enterica subsp. enterica	0.259 ± 0.308	1.248 ± 0.080	0.407 ± 0.447	Strong	Moderate	Strong
CHG55	Salmonella enterica subsp. enterica	0.341 ± 0.115	1.605 ± 0.066	0.395 ± 0.098	Strong	Weak	Strong
CHG31	Salmonella bongori	0.276 ± 0.037	0.561 ± 0.150	0.034 ± 0.012	Strong	Moderate	No biofilm
CHG32	Salmonella bongori	0.012 ± 0.007	0.422 ± 0.191	0.034 ± 0.017	No biofilm	No biofilm	No biofilm
CHG37	Salmonella bongori	0.030 ± 0.001	0.700 ± 0.204	0.066 ± 0.013	No biofilm	Weak	Weak
CHG39	Salmonella bongori	0.277 ± 0.094	1.075 ± 0.340	0.489 ± 0.192	Strong	Weak	Strong
CHG43	Salmonella bongori	0.077 ± 0.003	0.812 ± 0.288	0.129 ± 0.012	Moderate	Weak	Moderate
CHG53	Salmonella bongori	0.769 ± 0.205	1.244 ± 0.104	1.020 ± 0.207	Strong	Weak	Strong
Serovars
CHG4	Salmonella enterica ser. Weltevreden	0.138 ± 0.042	0.817 ± 0.273	1.509 ± 0.453	Strong	Strong	Strong
CHG5	Salmonella enterica ser. Chingola	0.181 ± 0.107	0.308 ± 0.055	0.446 ± 0.011	Strong	Weak	Strong
CHG6	Salmonella enterica ser. Arizonae	0.108 ± 0.090	0.287 ± 0.035	0.198 ± 0.044	Moderate	Weak	Strong
CHG19	Salmonella enterica ser. Arizonae	0.151 ± 0.045	0.574 ± 0.145	0.152 ± 0.036	Strong	Moderate	Moderate
CHG27	Salmonella enterica ser. Arizonae	0.026 ± 0.011	0.901 ± 0.040	0.472 ± 0.040	No biofilm	Strong	Strong
CHG33	Salmonella enterica ser. Arizonae	0.038 ± 0.017	0.848 ± 0.453	0.064 ± 0.031	Weak	Weak	Weak
CHG7	Salmonella enterica ser. Bovismorbificans	0.586 ± 0.116	0.220 ± 0.032	0.163 ± 0.147	Strong	No biofilm	Moderate
CHG10	Salmonella enterica ser. Typhimurium	1.028 ± 0.507	0.230 ± 0.059	0.167 ± 0.166	Strong	No biofilm	Moderate
CHG42	Salmonella enterica ser. Typhimurium	0.069 ± 0.064	0.089 ± 0.038	0.039 ± 0.018	Moderate	Weak	No biofilm
CHG47	Salmonella enterica ser. Typhimurium	0.024 ± 0.011	0.920 ± 0.315	0.053 ± 0.026	No biofilm	Weak	Weak
CHG49	Salmonella enterica ser. Typhimurium	0.167 ± 0.107	0.468 ± 0.142	0.163 ± 0.071	Strong	No biofilm	Moderate
CHG50	Salmonella enterica ser. Typhimurium	0.116 ± 0.084	0.310 ± 0.098	0.099 ± 0.007	Moderate	No biofilm	Weak
CHG51	Salmonella enterica ser. Typhimurium	0.098 ± 0.041	1.132 ± 0.333	0.185 ± 0.051	Moderate	Weak	Strong
CHG11	Salmonella enterica ser. Salamae	0.008 ± 0.004	0.284 ± 0.024	0.173 ± 0.019	No biofilm	Weak	Moderate
CHG12	Salmonella enterica ser. Houten	0.327 ± 0.059	0.360 ± 0.053	0.248 ± 0.118	Strong	Weak	Strong
CHG14	Salmonella enterica ser. Bareilly	0.182 ± 0.061	0.906 ± 0.163	1.009 ± 0.642	Strong	Strong	Strong
CHG18	Salmonella enterica ser. Heidelberg	1.048 ± 0.915	0.976 ± 0.104	0.064 ± 0.022	Strong	Strong	Weak
CHG28	Salmonella enterica ser. Heidelberg	0.098 ± 0.012	0.695 ± 0.167	0.038 ± 0.019	Weak	Strong	No biofilm
CHG21	Salmonella enterica ser. India	0.390 ± 0.091	1.024 ± 0.077	0.238 ± 0.094	Strong	Strong	Strong
CHG22	Salmonella enterica ser. Crossness	0.097 ± 0.008	0.640 ± 0.154	0.402 ± 0.366	Moderate	Moderate	Strong
CHG23	Salmonella enterica ser. Albany	0.212 ± 0.088	0.700 ± 0.108	0.303 ± 0.108	Strong	Moderate	Strong
CHG24	Salmonella enterica ser. Yovokome	0.107 ± 0.011	0.906 ± 0.277	0.041 ± 0.014	Weak	Strong	No biofilm
CHG25	Salmonella enterica ser. Pullorum	0.183 ± 0.082	0.733 ± 0.035	0.729 ± 0.082	Strong	Moderate	Strong
CHG26	Salmonella enterica ser. Infantis	0.320 ± 0.115	0.754 ± 0.124	0.743 ± 0.137	Strong	Moderate	Strong
CHG35	Salmonella enterica ser. Wandsworth	0.056 ± 0.018	0.723 ± 0.240	0.101 ± 0.031	Weak	Weak	Moderate
CHG38	Salmonella enterica ser. Kentucky	0.214 ± 0.088	1.012 ± 0.224	0.304 ± 0.255	Strong	Weak	Strong
CHG40	Salmonella enterica ser. Blockley	0.057 ± 0.030	0.387 ± 0.077	0.077 ± 0.037	Weak	No biofilm	Weak
CHG41	Salmonella enterica ser. Newport	0.245 ± 0.376	0.604 ± 0.310	0.388 ± 0.554	Strong	Weak	Strong
CHG44	Salmonella enterica ser. Manchester	0.078 ± 0.012	1.107 ± 0.172	0.128 ± 0.020	Moderate	Weak	Moderate
CHG52	Salmonella enterica ser. Koessen	0.206 ± 0.038	1.021 ± 0.169	0.290 ± 0.034	Strong	Weak	Strong
CHG54	Salmonella enterica ser. Blegdam	0.155 ± 0.078	0.584 ± 0.194	0.135 ± 0.027	Strong	Weak	Moderate
BLNK	Blank (LB broth)	0.089 ± 0.009	0.278 ± 0.017	0.0385 ± 0.036	–	–	–
CNTRL1	Negative control (un-inoculated broth)	0.025 ± 0.038	0.267 ± 0.002	0.023 ± 0.017	No biofilm	No biofilm	No biofilm
CNTRL2	Positive control (Salmonella enterica ser. Typhimurium ATCC 14028^TM)	0.352 ± 0.106	1.397 ± 0.107	0.493 ± 0.167	Strong	Moderate	Strong
CNTRL3	Positive control (Salmonella enterica ser. Enteritidis ATCC 13076^TM)	0.410 ± 0.017	1.725 ± 0.009	0.602 ± 0.059	Strong	Moderate	Strong
CNTRL4	Internal Control (E. coli 0157)	1.031 ± 0.072	1.236 ± 0.030	1.309 ± 0.076	Strong	Moderate	Strong

Pearson correlations between biofilm production potential of Salmonella serotypes incubated at varied temperatures.

Incubation temperatures		25°C	37°C	40°C
25°C	Pearson correlation	1	0.170^*	0.501^**
25°C	p-value		0.021	0.000
37°C	Pearson correlation	0.170^*	1	0.263^**
37°C	p-value	0.021		0.000
40°C	Pearson correlation	0.501^**	0.263^**	1
40°C	p-value	0.000	0.000

eISSN:: 2544-4646
Langue:: Anglais

Périodicité:: 4 fois par an
Sujets de la revue:: Life Sciences, Microbiology and Virology

RSS Feed de la revue

Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa

Article Category: original-paper

Publié en ligne: 23 nov. 2020

Pages: 427 - 439

Reçu: 10 août 2020

Accepté: 24 sept. 2020

DOI: https://doi.org/10.33073/pjm-2020-046

Mots clés
biofilm, biofilm production potential, crystal violet microtitre

© 2020 Stephen Abiola Akinola et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Identities of Salmonella isolates used for biofilm assay.

Optical densities and degree of biofilms formed by Salmonella serotypes as influenced by incubation temperatures.

Pearson correlations between biofilm production potential of Salmonella serotypes incubated at varied temperatures.

Biofilm Production Potential of Salmonella Serovars Isolated from Chickens in North West Province, South Africa

Article Category: original-paper

Publié en ligne: 23 nov. 2020

Pages: 427 - 439

Reçu: 10 août 2020

Accepté: 24 sept. 2020

DOI: https://doi.org/10.33073/pjm-2020-046

Mots clésbiofilm, biofilm production potential, crystal violet microtitre

© 2020 Stephen Abiola Akinola et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Identities of Salmonella isolates used for biofilm assay.

Optical densities and degree of biofilms formed by Salmonella serotypes as influenced by incubation temperatures.

Pearson correlations between biofilm production potential of Salmonella serotypes incubated at varied temperatures.

Mots clés
biofilm, biofilm production potential, crystal violet microtitre