<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Leptospirosis remains one of the most widespread zoonotic diseases in the world and Ukraine, in particular. Ukrainian clinicians have been faced with early detection of the disease due to the availability of only a serological method for routine diagnostics in Ukraine, namely the microscopic agglutination test (MAT). This paper demonstrates the first results of the complex application of MAT and polymerase chain reaction (PCR) for routine verification of leptospirosis, which were first applied simultaneously in Lviv Oblast of Ukraine in 2016. We examined the sera of 150 patients clinically suspected of leptospirosis, 31 of whom were treated at the Lviv Oblast Clinical Hospital for Infectious Diseases (LOCHID). The application of PCR during the first seven days of the disease allowed increasing the share of confirmed leptospirosis cases by 16,1% in patients that were treated in LOCHID during 2016–2017.</p></abstract>

Leptospirosis remains one of the most widespread zoonotic diseases in the world and Ukraine, in particular. Ukrainian clinicians have been faced with early detection of the disease due to the availability of only a serological method for routine diagnostics in Ukraine, namely the microscopic agglutination test (MAT). This paper demonstrates the first results of the complex application of MAT and polymerase chain reaction (PCR) for routine verification of leptospirosis, which were first applied simultaneously in Lviv Oblast of Ukraine in 2016. We examined the sera of 150 patients clinically suspected of leptospirosis, 31 of whom were treated at the Lviv Oblast Clinical Hospital for Infectious Diseases (LOCHID). The application of PCR during the first seven days of the disease allowed increasing the share of confirmed leptospirosis cases by 16,1% in patients that were treated in LOCHID during 2016–2017.

Application of PCR for Specific Diagnosis of Leptospirosis in Humans in Ukraine

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Application of PCR for Specific Diagnosis of Leptospirosis in Humans in Ukraine"}

Leptospirosis remains one of the most widespread zoonotic diseases in the world and Ukraine, in particular. Ukrainian clinicians have been faced with early...

Method of investigation	Total number of patients with suspected leptospirosis (n = 150)		Positive results by different methods		Total confirmation
Method of investigation	Number of the samples examined (absolute)	Number of the samples examined (%)	Number of positive samples (absolute)	Number of positive samples (%)	Number of positive samples (absolute)	Number of positive samples (%)
MAT	148	98.66	20	13.33	33	22
PCR	30	20	5	3.33
MAT + PCR	28	18.66	8	5.33

Application of PCR for Specific Diagnosis of Leptospirosis in Humans in Ukraine

Article Category: original-paper

Publié en ligne: 27 déc. 2020

Pages: 421 - 426

Reçu: 08 juil. 2020

Accepté: 29 sept. 2020

DOI: https://doi.org/10.33073/pjm-2020-045

Mots clés
Leptospirosis, diagnostics, microagglutination test, polymerase chain reaction

© 2020 Olena Zubach et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The diagnosis of patients with suspected leptospirosis.

Application of PCR for Specific Diagnosis of Leptospirosis in Humans in Ukraine

Article Category: original-paper

Publié en ligne: 27 déc. 2020

Pages: 421 - 426

Reçu: 08 juil. 2020

Accepté: 29 sept. 2020

DOI: https://doi.org/10.33073/pjm-2020-045

Mots clésLeptospirosis, diagnostics, microagglutination test, polymerase chain reaction

© 2020 Olena Zubach et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The diagnosis of patients with suspected leptospirosis.

Mots clés
Leptospirosis, diagnostics, microagglutination test, polymerase chain reaction