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Lactobacillus fermentum JX306 Restrain D-galactose-induced Oxidative Stress of Mice through its Antioxidant Activity

DI ZHANG
DI ZHANG
, 
CHUANG LI
CHUANG LI
, 
RUIRUI SHI
RUIRUI SHI
, 
FENGCHUN ZHAO
FENGCHUN ZHAO
 et 
ZHENGYOU YANG
ZHENGYOU YANG
   | 04 juin 2020
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Article Category: original-paper
Publié en ligne: 04 juin 2020
Pages: 205 - 215
Reçu: 14 févr. 2020
Accepté: 21 avr. 2020
DOI: https://doi.org/10.33073/pjm-2020-024
Mots clés
© 2020 Di Zhang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the liver of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the liver of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 2.

Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the kidney of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities, and MDA concentration in the kidney of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 3.

Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities and MDA concentration in the serum of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on GSH-Px, SOD, T-AOC activities and MDA concentration in the serum of mice with oxidative stress induced by D-galactose. (A) MDA; (B) GSH-Px; (C) SOD; (D) T-AOC. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 4.

Effect of L. fermentum JX306 on the expression of the genes encoding for peroxiredoxin-1 (Prdx1), glutathione peroxidase (Gsr), glutathione peroxidase (Gpx), and thioredoxin reductase (TR3) in the liver of D-galactose induced aging mice. (A) Thioredoxin reductase mRNA; (B) Peroxiredoxin1 mRNA; (C) Glutathione reductase mRNA; (D) Glutathione peroxidase mRNA. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).
Effect of L. fermentum JX306 on the expression of the genes encoding for peroxiredoxin-1 (Prdx1), glutathione peroxidase (Gsr), glutathione peroxidase (Gpx), and thioredoxin reductase (TR3) in the liver of D-galactose induced aging mice. (A) Thioredoxin reductase mRNA; (B) Peroxiredoxin1 mRNA; (C) Glutathione reductase mRNA; (D) Glutathione peroxidase mRNA. All data are presented as mean ± SD (n = 3). Bars with different letters were significantly different (p < 0.05).

Fig. 5.

Effects of L. fermentum JX306 treatment on organic damages in the liver (A), and the kidney (B) of D-galactose induced aging mice.
Effects of L. fermentum JX306 treatment on organic damages in the liver (A), and the kidney (B) of D-galactose induced aging mice.

The survival rate (%) of LAB in simulated bile, gastric fluid, and intestinal fluid.

Strains Simulated bile Simulated gastric fluid Simulated intestinal fluid
L. fermentum JX306 78.28 ± 0.18 a 53.05 ± 1.75 a 42.07 ± 6.52 a
L. fermentum GZ394 74.61 ± 4.67 a 30.57 ± 3.68 b 27.26 ± 4.95 b
L. plantarum SC34 65.09 ± 6.33 b 5.34 ± 1.92 c 12.23 ± 1.85 e
L. splantarum GZ328 74.48 ± 1.79 a 1.51 ± 0.22 e 9.75 ± 0.45 f
Pediococcus pentosaceus GZ430 22.99 ± 3.12 d 3.62 ± 1.11 d 15.26 ± 2.34 d
Leuconostoc mesenteroides YN295 33.89 ± 0.96 c 5.34 ± 0.79 c 17.28 ± 0.56 c

Groups of experimental animals.

Group Number Experimental treatments
Oral administration Intraperitoneal injection
NC 10 Normal saline (0.85%, 20 ml/kg BW) Normal saline (0.85%, 20 ml/kg BW)
MC 10 Normal saline (0.85%, 20 ml/kg BW) D-galactose (30 g/l, 200 mg/kg BW)
PC 10 Vitamin C (30 g/l, 200 mg/kg BW) D-galactose (30 g/l, 200 mg/kg BW)
LD 10 L. fermentum JX306 (108 CFU/day) D-galactose (30 g/l, 200 mg/kg BW)
MD 10 L. fermentum JX306 (109 CFU/day) D-galactose (30 g/l, 200 mg/kg BW)
HD 10 L. fermentum JX306 (1010 CFU/day) D-galactose (30 g/l, 200 mg/kg BW)

The selected LAB strains with high antioxidant activity.

Strains DPPH scavenging rate (%) Hydrogen radicals scavenging rate (%) Inhibition rate of lipid peroxidation (%)
L. fermentum JX306 37.29 ± 1.75 a 37.90 ± 0.29 a 28.14 ± 2.97 a
L. fermentum GZ394 34.92 ± 3.57 a 35.02 ± 1.70 b 23.89 ± 1.60 b
L. plantarum SC34 23.09 ± 4.00 c 34.50 ± 1.44 b 12.69 ± 0.23 d
L. plantarum GZ328 27.83 ± 2.25 b 29.21 ± 1.60 c 20.85 ± 2.07 bc
Pediococcus pentosaceus GZ430 16.22 ± 0.89 d 23.32 ± 1.62 d 18.01 ± 2.09 c
Leuconostoc mesenteroides YN295 15.26 ± 0.67 d 34.31 ± 1.18 b 14.43 ± 1.29 d
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