Plants | Type of extracts | Inhibition zone ratio of herbal extracts for | |||
---|---|---|---|---|---|
2A1a | 2A3 | 2A11 | 2V21 | ||
Aqueous extract | 5.33 ± 0.64 | 4.17 ± 0.38 | 4.67 ± 0.12 | 4.10 ± 0.44 | |
– | – | – | – | ||
EtOAc extract | 6.1 ± 0.19 | 5.6 ± 0.20 | 5.0 ± 0.23 | 6.1 ± 0.07 | |
MeOH extract | – | – | – | – | |
Acetone extract | – | – | – | – | |
Aqueous extract | 4.00 ± 0.46 | 4.10 ± 0.10 | 4.60 ± 0.53 | 3.80 ± 0.66 | |
1.9 ± 0.06 | 1.8 ± 0.15 | 1.6 ± 0.05 | 1.8 ± 0.17 | ||
EtOAc extract | 4.1 ± 0.11 | 4.3 ± 0.03 | 3.3 ± 0.05 | 3.8 ± 0.25 | |
MeOH extract | 2.5 ± 0.06 | 2.1 ± 0.25 | 2.4 ± 0.11 | 1.9 ± 0.15 | |
Acetone extract | 1.5 ± 0.25 | 1.4 ± 0.15 | 1.4 ± 0.36 | 1.5 ± 0.06 | |
Aqueous extract | 3.93 ± 0.15 | 3.47 ± 0.55 | 3.93 ± 0.21 | 3.50 ± 0.53 | |
3.5 ± 0.04 | 3.6 ± 0.24 | 3.6 ± 0.13 | 3.8 ± 0.14 | ||
EtOAc extract | – | – | – | – | |
MeOH extract | 4.9 ± 0.21 | 4.6 ± .17 | 4.0 ± 0.06 | 4.4 ± 0.08 | |
Acetone extract | 4.3 ± 0.12 | 4.4 ± 0.06 | 4.0 ± 0.15 | 4.1 ± 0.22 | |
Aqueous extract | 1.17 ± 0.40 | 0.97 ± 0.21 | 1.20 ± 0.36 | 0.93 ± 0.15 | |
1.9 ± 0.09 | 1.8 ± 0.06 | 1.5 ± 0.09 | 1.8 ± 0.21 | ||
EtOAc extract | 1.8 ± 0.14 | 1.9 ± 0.21 | 1.9 ± 0.06 | 1.5 ± 0.08 | |
MeOH extract | 1.5 ± .22 | 1.5 ± .19 | 1.4 ± 0.08 | 1.4 ± 0.11 | |
Acetone extract | 2.3 ± 0.12 | 1.8 ± 0.11 | 1.5 ± 0.13 | 1.8 ± 0.05 |
Sample No. | Symptoms | Site of isolation |
---|---|---|
01–04 | Deformed and yellowish colored hepatopancreas | Hepatopancreas |
05–06 | Blackish colored hepatopancreas | Hepatopancreas |
07–11 | Discolored hepatopancreas | Hepatopancreas |
12–13 | Yellowish colored hepatopancreas | Hepatopancreas |
14–15 | No visible symptom | Hepatopancreas |
Test Type | Test | Characteristics |
---|---|---|
Colony characteristics | Color in NA media | Brownish |
Color in TCBS media | Yellowish | |
Size | Large | |
Shape | Round | |
Elevation | Convex | |
Morphological characteristics | Shape | Comma |
Motility | + | |
Growth in 0% NaCl | – | |
Growth in 2,4 and 8% NaCl containing media | + | |
Growth at 4°C | – | |
Growth at 40°C | + | |
Biochemical characteristics | Gram’s staining | – |
Oxidative-Fermentative | F | |
Oxidase | + | |
Catalase | + | |
Acetoin production | – | |
H2S production | – | |
Indole | + | |
Sensitivity to a vibriostatic agent 0/129 | + | |
Arginine dihydrolase | – | |
Lysine decarboxylase | + | |
Acid production from | Glucose | + |
Arabinose | – | |
Manitol | + | |
Sorbitol | + | |
Sucrose | + |
Isolates | Inhibition zone ratio against different antibiotics | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Er | Pe | Am | Va | Amp | Le | Cx | Az | Ni | Ce | Ge | |
2A1a | R | R | R | R | R | 7.3 ± 0.2 | 4.6 ± 0.3 | 3.8 ± 0.3 | 6.8 ± 0.1 | R | 6 ± 0.2 |
2A3 | R | R | R | R | R | 7.3 ± 0.3 | 3.7 ± 0.2 | 3.3 ± 0.1 | 7 ± 0.1 | R | 5.7 ± 0.1 |
2A11 | R | R | R | R | R | 7.2 ± 0.1 | 4.8 ± 0.6 | 3.7 ± 0.6 | 6.5 ± 0.5 | R | 5.8 ± 0.1 |
2V21 | R | R | R | R | R | 7.0 ± 0.1 | 4.2 ± 0.2 | 3.3 ± 0.2 | 7.0 ± 0.2 | R | 5.7 ± 0.3 |
Sl. No. | English name | Scientific name | Plant parts used |
---|---|---|---|
1 | Zinger | Rhizome | |
2 | Turmeric | Rhizome | |
3 | Clove | Bud | |
4 | Garlic | Bulb | |
5 | Onion | Bulb | |
6 | Black cumin | Seed | |
7 | Mehogoni | Seed | |
8 | Bottle gourd | Seed, Fruit | |
9 | Guava | Fruit | |
10 | Olive | Fruit | |
11 | Chilli | Fruit | |
12 | Rose periwinkle | Leaf and flower | |
13 | Amla | Leaf | |
14 | Tamarind | Leaf | |
15 | Arjun | Leaf | |
16 | Papaya | Leaf | |
17 | Carunda | Leaf | |
18 | Bermuda grass | Leaf | |
19 | Neem | Leaf | |
20 | Pomegranate | Leaf | |
21 | Carambola | Leaf |