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Effects of Sodium Tripolyphosphate on Oral Commensal and Pathogenic Bacteria

JI-HOI MOON
JI-HOI MOON
, 
MI HEE NOH
MI HEE NOH
, 
EUN-YOUNG JANG
EUN-YOUNG JANG
, 
SEOK BIN YANG
SEOK BIN YANG
, 
SANG WOOK KANG
SANG WOOK KANG
, 
KYU HWAN KWACK
KYU HWAN KWACK
, 
JAE-IN RYU
JAE-IN RYU
 et 
JIN-YONG LEE
JIN-YONG LEE
   | 28 juin 2019
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Article Category: original-paper
Publié en ligne: 28 juin 2019
Pages: 263 - 268
Reçu: 10 janv. 2019
Accepté: 22 avr. 2019
DOI: https://doi.org/10.33073/pjm-2019-029
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© 2019 Ji-Hoi Moon MATEJ REMENÁR et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

MIC determination of polyP3 by the agar dilution method. The bacterial cells were spot-inoculated (approximately 105 to 106 cells/spot) onto Brucella blood agar plates containing polyP3 at various concentrations and incubated at 37°C for 3 days anaerobically. The MIC was defined as the lowest concentration that inhibited the bacterial growth on the plate. The results for P. intermedia ATCC 49046 are the same as those reported in our previous study (Jang et al. 2016).
MIC determination of polyP3 by the agar dilution method. The bacterial cells were spot-inoculated (approximately 105 to 106 cells/spot) onto Brucella blood agar plates containing polyP3 at various concentrations and incubated at 37°C for 3 days anaerobically. The MIC was defined as the lowest concentration that inhibited the bacterial growth on the plate. The results for P. intermedia ATCC 49046 are the same as those reported in our previous study (Jang et al. 2016).

Fig. 2.

Effect of polyP3 on the growth of the bacteria at a high inoculum density. Each bacterial strain cultured to exponential phase was adjusted to approximately 108 cells/ml in B-HK, then exposed to polyP3 at various concentrations. The bacterial growth was measured by reading optical density at 600 nm (OD600) after the 24-hour incubation. Data are means ± SDs from two independent experiments performed in triplicate. One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3).
Effect of polyP3 on the growth of the bacteria at a high inoculum density. Each bacterial strain cultured to exponential phase was adjusted to approximately 108 cells/ml in B-HK, then exposed to polyP3 at various concentrations. The bacterial growth was measured by reading optical density at 600 nm (OD600) after the 24-hour incubation. Data are means ± SDs from two independent experiments performed in triplicate. One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3).

Fig. 3.

Effect of polyP3 on the growth and biofilm formation of the four bacterial species in a mixed culture. (A) To assess the total growth (planktonic and biofilm bacterial growth), the biofilm bacterial cells were dispersed, mixed thoroughly with planktonic bacterial cells, and OD600 of the suspension was measured. The suspension was diluted 2 to 10 times, and OD600 of the diluted suspension was also measured. The amount of biofilm formed by the mixed bacterial species was quantitated by crystal violet staining. Readings were expressed as mean %OD600 (%OD600 = OD600 ∕ mean OD600 of control × 100). One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3). (B) SEM images of the biofilms at a magnification of 20 000 × (left) and 100 000 × (right).
Effect of polyP3 on the growth and biofilm formation of the four bacterial species in a mixed culture. (A) To assess the total growth (planktonic and biofilm bacterial growth), the biofilm bacterial cells were dispersed, mixed thoroughly with planktonic bacterial cells, and OD600 of the suspension was measured. The suspension was diluted 2 to 10 times, and OD600 of the diluted suspension was also measured. The amount of biofilm formed by the mixed bacterial species was quantitated by crystal violet staining. Readings were expressed as mean %OD600 (%OD600 = OD600 ∕ mean OD600 of control × 100). One-way ANOVA with Tukey’s post-hoc tests p < 0.001, versus control (0% polyP3). (B) SEM images of the biofilms at a magnification of 20 000 × (left) and 100 000 × (right).
eISSN:
2544-4646
Langue:
Anglais
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Sujets de la revue:
Life Sciences, Microbiology and Virology
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