<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Gastric microbiota provides a biological barrier against the invasion of foreign pathogens from the oral cavity, playing a vital role in maintaining gastrointestinal health. <italic>Klebsiella</italic> spp. of oral origin causes various infections not only in gastrointestinal tract but also in other organs, with <italic>Klebsiella pneumoniae</italic> serotype K1 resulting in a liver abscess (KLA) through oral inoculation in mice. However, the relationship between gastric microbiota and the extra-gastrointestinal KLA infection is not clear. In our study, a 454 pyrosequencing analysis of the bacterial 16S rRNA gene shows that the composition of gastric mucosal microbiota in mice with or without KLA infection varies greatly after oral inoculation with <italic>K. pneumoniae</italic> serotype K1 isolate. Interestingly, only several bacteria taxa show a significant change in gastric mucosal microbiota of KLA mice, including the decreased abundance of <italic>Bacteroides, Alisptipes</italic> and increased abundance of <italic>Streptococcus</italic>. It is worth noting that the abundance of <italic>Klebsiella</italic> exhibits an obvious increase in KLA mice, which might be closely related to KLA infection. At the same time, the endogenous antibiotics, defensins, involved in the regulation of the bacterial microbiota also show an increase in stomach and intestine. All these findings indicate that liver abscess caused by <italic>K. pneumoniae</italic> oral inoculation has a close relationship with gastric microbiota, which might provide important information for future clinical treatment.</p></abstract>

Gastric microbiota provides a biological barrier against the invasion of foreign pathogens from the oral cavity, playing a vital role in maintaining gastrointestinal health. Klebsiella spp. of oral origin causes various infections not only in gastrointestinal tract but also in other organs, with Klebsiella pneumoniae serotype K1 resulting in a liver abscess (KLA) through oral inoculation in mice. However, the relationship between gastric microbiota and the extra-gastrointestinal KLA infection is not clear. In our study, a 454 pyrosequencing analysis of the bacterial 16S rRNA gene shows that the composition of gastric mucosal microbiota in mice with or without KLA infection varies greatly after oral inoculation with K. pneumoniae serotype K1 isolate. Interestingly, only several bacteria taxa show a significant change in gastric mucosal microbiota of KLA mice, including the decreased abundance of Bacteroides, Alisptipes and increased abundance of Streptococcus. It is worth noting that the abundance of Klebsiella exhibits an obvious increase in KLA mice, which might be closely related to KLA infection. At the same time, the endogenous antibiotics, defensins, involved in the regulation of the bacterial microbiota also show an increase in stomach and intestine. All these findings indicate that liver abscess caused by K. pneumoniae oral inoculation has a close relationship with gastric microbiota, which might provide important information for future clinical treatment.

Gastric Microbiota Alteration in <italic>Klebsiella pneumoniae</italic>-Caused Liver Abscesses Mice

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Gastric Microbiota Alteration in -Caused Liver Abscesses Mice

Gastric microbiota provides a biological barrier against the invasion of foreign pathogens from the oral cavity, playing a vital role in maintaining...

Gastric Microbiota Alteration in Klebsiella pneumoniae-Caused Liver Abscesses Mice

Article Category: original-paper

Publié en ligne: 28 juin 2019

Pages: 247 - 254

Reçu: 16 janv. 2019

Accepté: 24 mars 2019

DOI: https://doi.org/10.33073/pjm-2019-026

Mots clés
serotype K1, defensins, gastric mucosal microbiota, High-throughput pyrosequencing, liver abscess

© 2019 Nan Chen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Gastric Microbiota Alteration in Klebsiella pneumoniae-Caused Liver Abscesses Mice

Article Category: original-paper

Publié en ligne: 28 juin 2019

Pages: 247 - 254

Reçu: 16 janv. 2019

Accepté: 24 mars 2019

DOI: https://doi.org/10.33073/pjm-2019-026

Mots clésserotype K1, defensins, gastric mucosal microbiota, High-throughput pyrosequencing, liver abscess

© 2019 Nan Chen et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Mots clés
serotype K1, defensins, gastric mucosal microbiota, High-throughput pyrosequencing, liver abscess