<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> An HPLC method with fluorescence detection (HPLC-FLD) was developed for the simultaneous quantitative determination of enrofloxacin, ciprofloksacin, sarafloxacin, and difloxacin in feed. The samples were extracted with 0.25% formic acid in ultrasonic bath and purified by solid phase extraction (SPE) on Strata-X cartridges. The samples were analysed on Luna C8 liquid chromatography column with gradient programme by acetonitrile and 0.025 M phosphoric acid with 0.0025 M sodium 1- heptanesulfonate monohydrate. The method was successfully validated according to the requirements of the European Decision 2002/657/EC. Recoveries of the components from spiked feed samples ranged from 55%-70%. Repeatability was &lt;7%. The method presented here proved to be efficient, rapid, and a selective approach for determination of fluoroquinolones in feed.</p>
</abstract>

An HPLC method with fluorescence detection (HPLC-FLD) was developed for the simultaneous quantitative determination of enrofloxacin, ciprofloksacin, sarafloxacin, and difloxacin in feed. The samples were extracted with 0.25% formic acid in ultrasonic bath and purified by solid phase extraction (SPE) on Strata-X cartridges. The samples were analysed on Luna C8 liquid chromatography column with gradient programme by acetonitrile and 0.025 M phosphoric acid with 0.0025 M sodium 1- heptanesulfonate monohydrate. The method was successfully validated according to the requirements of the European Decision 2002/657/EC. Recoveries of the components from spiked feed samples ranged from 55%-70%. Repeatability was <7%. The method presented here proved to be efficient, rapid, and a selective approach for determination of fluoroquinolones in feed.

Simultaneous Determinaton of Fluoroquinolones in Feed by Liquid Chromatography with Fluorescence Detection

Department of Pharmacology and Toxicology

Journal of Veterinary Research

An HPLC method with fluorescence detection (HPLC-FLD) was developed for the simultaneous quantitative determination of enrofloxacin, ciprofloksacin,...

Simultaneous Determinaton of Fluoroquinolones in Feed by Liquid Chromatography with Fluorescence Detection

Publié en ligne: 17 janv. 2013

Pages: 343 - 347

DOI: https://doi.org/10.2478/v10213-012-0060-y

Mots clés
feed, fluoroquinolones, residues, liquid chromatography

This content is open access.

Simultaneous Determinaton of Fluoroquinolones in Feed by Liquid Chromatography with Fluorescence Detection

Publié en ligne: 17 janv. 2013

Pages: 343 - 347

DOI: https://doi.org/10.2478/v10213-012-0060-y

Mots clésfeed, fluoroquinolones, residues, liquid chromatography

This content is open access.

Mots clés
feed, fluoroquinolones, residues, liquid chromatography