<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml">The study discusses the problem of role of various substances in the mechanisms of contraction and relaxation of human term pregnant myometrium in the experimental <italic>in vitro</italic> conditions.Human myometrium tissue samples were collected from term pregnant women who had to undergo Caesarean section. The effects of potassium ion channels openers and closers, phosphodiesterase inhibitors and tachykinins were measured in the reactivity of human term pregnant myometrium in the experimental <italic>in vitro</italic> conditions.Specific agonist-opener of KATP potassium ion channels - pinacidil significantly reduced contractile activity of human term pregnant myometrial strips induced by administration of oxytocin in <italic>in vitro</italic> conditions, while the effect of specific agonist-opener of BKCa2+ potassium ion channels - NS1619 was not significant. Glibenclamide, specific KATP potassium ion channels antagonist-closer significantly increased contractile activity of human term pregnant myometrial strips in <italic>in vitro</italic> conditions. The contractile effect of specific antagonist-closer of BKCa2+ potassium ion channels - tetraethylammonium was not significant. Phosphodiesterase-4 inhibitor - rolipram and phosphodiesterase-5 inhibitor - sildenaphil led to statistically significant decrease of oxytocin-induced contractile activity in human term pregnant myometrial strips in <italic>in vitro</italic> conditions. Tachykinins - neurokinin A, neurokinin B and substance P markedly stimulated the activity of the human term pregnant myometrial strips in <italic>in vitro</italic> conditions.The results showed the different effectiveness of potassium ion channels openers and closers, phosphodiesterase inhibitors as well as tachykinins in the mechanisms of contraction and relaxation of human term pregnant myometrium in <italic>in vitro</italic> conditions.</abstract>

The study discusses the problem of role of various substances in the mechanisms of contraction and relaxation of human term pregnant myometrium in the experimental in vitro conditions.Human myometrium tissue samples were collected from term pregnant women who had to undergo Caesarean section. The effects of potassium ion channels openers and closers, phosphodiesterase inhibitors and tachykinins were measured in the reactivity of human term pregnant myometrium in the experimental in vitro conditions.Specific agonist-opener of KATP potassium ion channels - pinacidil significantly reduced contractile activity of human term pregnant myometrial strips induced by administration of oxytocin in in vitro conditions, while the effect of specific agonist-opener of BKCa2+ potassium ion channels - NS1619 was not significant. Glibenclamide, specific KATP potassium ion channels antagonist-closer significantly increased contractile activity of human term pregnant myometrial strips in in vitro conditions. The contractile effect of specific antagonist-closer of BKCa2+ potassium ion channels - tetraethylammonium was not significant. Phosphodiesterase-4 inhibitor - rolipram and phosphodiesterase-5 inhibitor - sildenaphil led to statistically significant decrease of oxytocin-induced contractile activity in human term pregnant myometrial strips in in vitro conditions. Tachykinins - neurokinin A, neurokinin B and substance P markedly stimulated the activity of the human term pregnant myometrial strips in in vitro conditions.The results showed the different effectiveness of potassium ion channels openers and closers, phosphodiesterase inhibitors as well as tachykinins in the mechanisms of contraction and relaxation of human term pregnant myometrium in in vitro conditions.

Mechanisms of Contraction and Relaxation in Human Term Pregnant Myometrium

Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, Martin

Clinic of Obstetrics and Gynecology, Jessenius Faculty of Medicine and Martin University Hospital, Martin, Slovak Republic

Acta Medica Martiniana

{"article-title":"Mechanisms of Contraction and Relaxation in Human Term Pregnant Myometrium"}

The study discusses the problem of role of various substances in the mechanisms of contraction and relaxation of human term pregnant myometrium in the...

Mechanisms of Contraction and Relaxation in Human Term Pregnant Myometrium

Publié en ligne: 06 mars 2012

Pages: 38 - 44

DOI: https://doi.org/10.2478/v10201-011-0026-4

Mots clés
phosphodiesterase inhibitors, potassium ion channels openers and closers, tachykinins

This content is open access.

Mechanisms of Contraction and Relaxation in Human Term Pregnant Myometrium

Publié en ligne: 06 mars 2012

Pages: 38 - 44

DOI: https://doi.org/10.2478/v10201-011-0026-4

Mots clésphosphodiesterase inhibitors, potassium ion channels openers and closers, tachykinins

This content is open access.

Mots clés
phosphodiesterase inhibitors, potassium ion channels openers and closers, tachykinins