<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Our objectives in the present study were to determine the activity of monoamine oxidases A and B (MAO AB) in rat ovary, oviduct and uterus during preimplantation period of pregnancy. It should help us to clarify and better understand possible involvement of both MAO enzymes in the reproductive process. Pregnant females were killed employing a lethal dose of thiopental on the first (D1), on the third (D3), and on the fifth (D5) days of pregnancy. Rats were perfused transcardially with the PBS to rinse out of the body as much blood as possible. Ovaries, oviducts and uteri were immediately removed and stored until the measurement was done. MAO activity was determined by fluorescent monoamine oxidase detection kit. In the ovaries we have found the highest MAO activity at D3, followed by D1, and the lowest levels were recorded at D5 of pregnancy. In the oviducts, the highest MAO activity was detected again at D3, followed by D5, and by D1 of pregnancy. But statistical analysis did not reveal any difference between individual days of pregnancy nor in the ovaries, neither in uterine tubes. Uteri were the only organs, in which statistically significant differences were detected (p&lt;0.001). The highest activity of MAO was observed at D5, followed by D1, and by D3 of pregnancy. Potential mechanisms responsible for the changed MAO activity in gonads during preimplantation period of pregnancy are proposed.</p></abstract>

Our objectives in the present study were to determine the activity of monoamine oxidases A and B (MAO AB) in rat ovary, oviduct and uterus during preimplantation period of pregnancy. It should help us to clarify and better understand possible involvement of both MAO enzymes in the reproductive process. Pregnant females were killed employing a lethal dose of thiopental on the first (D1), on the third (D3), and on the fifth (D5) days of pregnancy. Rats were perfused transcardially with the PBS to rinse out of the body as much blood as possible. Ovaries, oviducts and uteri were immediately removed and stored until the measurement was done. MAO activity was determined by fluorescent monoamine oxidase detection kit. In the ovaries we have found the highest MAO activity at D3, followed by D1, and the lowest levels were recorded at D5 of pregnancy. In the oviducts, the highest MAO activity was detected again at D3, followed by D5, and by D1 of pregnancy. But statistical analysis did not reveal any difference between individual days of pregnancy nor in the ovaries, neither in uterine tubes. Uteri were the only organs, in which statistically significant differences were detected (p<0.001). The highest activity of MAO was observed at D5, followed by D1, and by D3 of pregnancy. Potential mechanisms responsible for the changed MAO activity in gonads during preimplantation period of pregnancy are proposed.

Activity of Monoamine Oxidases in Rat Female Genital Organs During Preimplantation Period of Pregnancy

Department of Anatomy, Medical Faculty, P.J. Safarik University, Kosice, Slovak Republic

Neurobiological Institute, Slovak Academy of Sciences, Kosice, Slovak Republic

Acta Medica Martiniana

Our objectives in the present study were to determine the activity of monoamine oxidases A and B (MAO AB) in rat ovary, oviduct and uterus during...

Activity of Monoamine Oxidases in Rat Female Genital Organs During Preimplantation Period of Pregnancy

Publié en ligne: 03 juin 2011

Pages: 16 - 22

DOI: https://doi.org/10.2478/v10201-011-0002-z

Mots clés
MAO, ovary, oviduct, uterus

This content is open access.

Activity of Monoamine Oxidases in Rat Female Genital Organs During Preimplantation Period of Pregnancy

Publié en ligne: 03 juin 2011

Pages: 16 - 22

DOI: https://doi.org/10.2478/v10201-011-0002-z

Mots clésMAO, ovary, oviduct, uterus

This content is open access.

Mots clés
MAO, ovary, oviduct, uterus