<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of this study was to develop a robust method for the simultaneous determination of the activities of three porcine CYP450 enzymes in hepatic microsomes. A cocktail consisting of three selective CYP450 probe substrates, 7-ethoxyresorufin (CYP1A), coumarin (CYP2A) and 7-benzyloxy-4-trifluoromethylcoumarin (BFC; CYP3A), was incubated with porcine liver microsomes. The presence of 7-ethoxyresorufin appears to significantly influence the kinetics of coumarin hydroxylation and BFC <italic>O</italic>-debenzylation. These results indicate that the use of 7-ethoxyresorufin in substrate cocktails together with coumarin and BFC should be avoided.</p></abstract>

The aim of this study was to develop a robust method for the simultaneous determination of the activities of three porcine CYP450 enzymes in hepatic microsomes. A cocktail consisting of three selective CYP450 probe substrates, 7-ethoxyresorufin (CYP1A), coumarin (CYP2A) and 7-benzyloxy-4-trifluoromethylcoumarin (BFC; CYP3A), was incubated with porcine liver microsomes. The presence of 7-ethoxyresorufin appears to significantly influence the kinetics of coumarin hydroxylation and BFC O-debenzylation. These results indicate that the use of 7-ethoxyresorufin in substrate cocktails together with coumarin and BFC should be avoided.

Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes

Department of Food Science, BioCenter Swedish University of Agricultural Sciences PO Box 7051, SE-750 07, Uppsala, Sweden TEL: +46-18-67 20 41 • FAX: +4618672995

University of Veterinary and Pharmaceutical Science Brno, Faculty of Hygiene and Ecology, Department of Meat Hygiene and Technology, Brno, Czech Republic

Department of Food Science, BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden

Interdisciplinary Toxicology

The aim of this study was to develop a robust method for the simultaneous determination of the activities of three porcine CYP450 enzymes in hepatic...

Simultaneous determination of cytochrome P450 1A, 2A and 3A activities in porcine liver microsomes

Publié en ligne: 16 janv. 2013

Pages: 150 - 154

DOI: https://doi.org/10.2478/v10102-012-0024-3

This content is open access.