<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Authors evaluated the incidence of total chromosomal aberrations (CA) and their types - chromatid-type (CTA) and chromosome-type (CSA) in peripheral blood lymphocytes from 72 oncologic unit's workers occupationally exposed to cytostatics in relationship to polymorphisms of DNA repair genes <italic>XPD, XPG</italic> and <italic>XPC</italic>. The cytogenetic analysis was used for determination of chromosomal aberrations frequency and PCR-RFLP method for polymorphisms of genes. Statistically higher frequency of total CA was detected in exposed group as compared to control (1.90±1.34% vs. 1.26±0.93%; Mann-Whitney U-test, <italic>p</italic>=0.001). There was not detected any difference between CTA and CSA (0.92±1.04% vs. 0.98±1.17%). Similarly, in genes <italic>XPD</italic> exon 23 and <italic>XPC</italic> exon 15 wasn't detected any difference neither in total chromosomal aberrations nor in CTA and CSA types. Statistically significant decrease of total chromosomal aberrations and CTA-type with presence of variant allele C was detected in gene <italic>XPG</italic> exon 15. Authors pointed out the importance of individual susceptibility factors in evaluation of effects of genotoxic agents, in that event, when the concentration does not meet the occupational exposure limit.</p></abstract>

Authors evaluated the incidence of total chromosomal aberrations (CA) and their types - chromatid-type (CTA) and chromosome-type (CSA) in peripheral blood lymphocytes from 72 oncologic unit's workers occupationally exposed to cytostatics in relationship to polymorphisms of DNA repair genes XPD, XPG and XPC. The cytogenetic analysis was used for determination of chromosomal aberrations frequency and PCR-RFLP method for polymorphisms of genes. Statistically higher frequency of total CA was detected in exposed group as compared to control (1.90±1.34% vs. 1.26±0.93%; Mann-Whitney U-test, p=0.001). There was not detected any difference between CTA and CSA (0.92±1.04% vs. 0.98±1.17%). Similarly, in genes XPD exon 23 and XPC exon 15 wasn't detected any difference neither in total chromosomal aberrations nor in CTA and CSA types. Statistically significant decrease of total chromosomal aberrations and CTA-type with presence of variant allele C was detected in gene XPG exon 15. Authors pointed out the importance of individual susceptibility factors in evaluation of effects of genotoxic agents, in that event, when the concentration does not meet the occupational exposure limit.

Effect of occupational exposure to cytostatics and nucleotide excision repair polymorphism on chromosomal aberrations frequency

Institute of Medical Biology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia

Clinic of Occupational Medicine and Toxicology, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia

Institute of Experimental Medicine Academy of Sciences, Prague, Czech Republic

National Institute of Public Health, Prague, Czech Republic

Institute of Public Health, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia

Interdisciplinary Toxicology

Authors evaluated the incidence of total chromosomal aberrations (CA) and their types - chromatid-type (CTA) and chromosome-type (CSA) in peripheral blood...

Effect of occupational exposure to cytostatics and nucleotide excision repair polymorphism on chromosomal aberrations frequency

Publié en ligne: 19 juin 2009

Pages: 13 - 17

DOI: https://doi.org/10.2478/v10102-009-0002-6

Mots clés
chromosomal aberrations, cytostatics, occupational exposure, DNA repair genes, polymorphisms of genes

This content is open access.

Effect of occupational exposure to cytostatics and nucleotide excision repair polymorphism on chromosomal aberrations frequency

Publié en ligne: 19 juin 2009

Pages: 13 - 17

DOI: https://doi.org/10.2478/v10102-009-0002-6

Mots cléschromosomal aberrations, cytostatics, occupational exposure, DNA repair genes, polymorphisms of genes

This content is open access.

Mots clés
chromosomal aberrations, cytostatics, occupational exposure, DNA repair genes, polymorphisms of genes