<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Our previous research showed that the best yield of virus-like particles (VLPs) formed by RNA bacteriophage GA coat protein was obtained by expression in yeast Pichia pastoris, while other used expression systems in Saccharomyces cerevisiae gave much lower amounts of capsids. The main reasons to attempt further studies in Saccharomyces cerevisiae were to improve the yield of GA-based VLPs using constructs with optimised nucleotide triplets in coding sequences, and to exploit the possibilities of the two-promoter Gal1/Gal10 system of expression vector pESC-URA for production of the desired mosaic VLPs and for packaging of mRNAs into VLPs in vivo</p></abstract>

Our previous research showed that the best yield of virus-like particles (VLPs) formed by RNA bacteriophage GA coat protein was obtained by expression in yeast Pichia pastoris, while other used expression systems in Saccharomyces cerevisiae gave much lower amounts of capsids. The main reasons to attempt further studies in Saccharomyces cerevisiae were to improve the yield of GA-based VLPs using constructs with optimised nucleotide triplets in coding sequences, and to exploit the possibilities of the two-promoter Gal1/Gal10 system of expression vector pESC-URA for production of the desired mosaic VLPs and for packaging of mRNAs into VLPs in vivo

Expression of GA Coat Protein-Derived Mosaic Virus-Like Particles in Saccharomyces cerevisiae and Packaging in vivo of mRNAs into Particles

Biomedical Research and Study Centre, Rātsupites ielā 1, Rigā, LV-1067, LATVIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

Our previous research showed that the best yield of virus-like particles (VLPs) formed by RNA bacteriophage GA coat protein was obtained by expression in yeast...

Expression of GA Coat Protein-Derived Mosaic Virus-Like Particles in Saccharomyces cerevisiae and Packaging in vivo of mRNAs into Particles

Publié en ligne: 01 mai 2013

Pages: 234 - 241

DOI: https://doi.org/10.2478/v10046-012-0015-y

This content is open access.