<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Frequency of callusogenesis, type and capacity of regeneration, cell ploidy, DNA methylation level, histological features, autofluorescence and photobleaching effects were studied in flax calli grown on medium supplemented with different concentrations of carbon (C) nanoparticles. The presence of carbon nanoparticles on medium decreased the percentage calli formation. Detailed histological study led to better understanding of changes in calli caused by nanoparticles. Ploidy variation in calli cells significantly depended on carbon nanoparticle concentration in the medium. Different types of calli regeneration associated with different C nanoparticles concentration in medium were observed: somatic embryogenesis (direct or indirect) or organogenesis. C nanoparticle dose-dependent DNA hypermethylation in flax calli cells were found. Increased repair ability during laser irradiation was found in calli grown on medium with carbon nanoparticles</p></abstract>

Frequency of callusogenesis, type and capacity of regeneration, cell ploidy, DNA methylation level, histological features, autofluorescence and photobleaching effects were studied in flax calli grown on medium supplemented with different concentrations of carbon (C) nanoparticles. The presence of carbon nanoparticles on medium decreased the percentage calli formation. Detailed histological study led to better understanding of changes in calli caused by nanoparticles. Ploidy variation in calli cells significantly depended on carbon nanoparticle concentration in the medium. Different types of calli regeneration associated with different C nanoparticles concentration in medium were observed: somatic embryogenesis (direct or indirect) or organogenesis. C nanoparticle dose-dependent DNA hypermethylation in flax calli cells were found. Increased repair ability during laser irradiation was found in calli grown on medium with carbon nanoparticles

Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Institute of Systematic Biology, Daugavpīls University, Vienības iela 13, Daugavpīls, LV-5401, LATVIA

G. Liberts’ Innovative Microscopy Centre, Daugavpils University, Parādes iela 1, Daugavpīls, LV-5401, LATVIA

Institute of Biology, University of Latvia, Miera iela 3, Salaspils, LV-2169, LATVIA

Animal Health and Environment “BIOR”, Institute of Food Safety, Lejupes iela 3, Rîga, LV-1076, LATVIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

Frequency of callusogenesis, type and capacity of regeneration, cell ploidy, DNA methylation level, histological features, autofluorescence and photobleaching...

Reaction of Flax (Linum Usitatissimum L.) Calli Culture to Supplement of Medium by Carbon Nanoparticles

Publié en ligne: 12 avr. 2013

Pages: 200 - 209

DOI: https://doi.org/10.2478/v10046-012-0010-3

This content is open access.