<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Infection with M. tuberculosis (MT) is difficult to diagnose in young BCG (Bacillus Calmette-Guérin) vaccinated children using <italic>Mantoux</italic> test alone, as a positive test result may be due to infection with MT and previous BCG vaccination. We aimed to test the T-SPOT. <italic>TB</italic> test for BCG-vaccinated children aged five and younger in two groups — with or without contact with an active tuberculosis (ATB) patient. Prospectively a study group of 121 children (having contact with ATB patient) and a control group of 64 children (without known contact with ATB patient) were examined using <italic>Mantoux</italic> and T-SPOT. <italic>TB</italic> tests. The T-SPOT. <italic>TB</italic> test was positive in 66 (54.5%) study group children and in 2 (3.1%) control group children (P &lt; 0.01). Induration in the <italic>Mantoux</italic> test ≥ 10 mm was observed in 62 (91.0%) of 68 T-SPOT. <italic>TB</italic> positive children, and 34 (29.1%) of 117 T-SPOT. <italic>TB</italic> negative children (P &lt; 0.01). In the group with a negative T-SPOT. <italic>TB</italic> result boosting of the <italic>Mantoux</italic> test was observed in 21 (66%) of 32 children who had received repeated <italic>Mantoux</italic> testing before being included in the study. According to the results, the application of the T-SPOT. <italic>TB</italic> test is reasonable for primary contact children evaluation and follow-up contact children for whom primary tests did not confirm infection with MT.</p></abstract>

Infection with M. tuberculosis (MT) is difficult to diagnose in young BCG (Bacillus Calmette-Guérin) vaccinated children using Mantoux test alone, as a positive test result may be due to infection with MT and previous BCG vaccination. We aimed to test the T-SPOT. TB test for BCG-vaccinated children aged five and younger in two groups — with or without contact with an active tuberculosis (ATB) patient. Prospectively a study group of 121 children (having contact with ATB patient) and a control group of 64 children (without known contact with ATB patient) were examined using Mantoux and T-SPOT. TB tests. The T-SPOT. TB test was positive in 66 (54.5%) study group children and in 2 (3.1%) control group children (P < 0.01). Induration in the Mantoux test ≥ 10 mm was observed in 62 (91.0%) of 68 T-SPOT. TB positive children, and 34 (29.1%) of 117 T-SPOT. TB negative children (P < 0.01). In the group with a negative T-SPOT. TB result boosting of the Mantoux test was observed in 21 (66%) of 32 children who had received repeated Mantoux testing before being included in the study. According to the results, the application of the T-SPOT. TB test is reasonable for primary contact children evaluation and follow-up contact children for whom primary tests did not confirm infection with MT.

Using of T-Spot.<italic>TB</italic> and <italic>Mantoux</italic> tests in diagnosis of <italic>M. tuberculosis</italic> infection in BCG vaccinated children aged five and younger

State Agency of Tuberculosis and Lung Diseases of Latvia, p.o. Cekule, Rīgas raj., LV-2118, LATVIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

Using of T-Spot. and  tests in diagnosis of  infection in BCG vaccinated children aged five and younger

Infection with M. tuberculosis (MT) is difficult to diagnose in young BCG (Bacillus Calmette-Guérin) vaccinated children using Mantoux test alone, as a...

Using of T-Spot.TB and Mantoux tests in diagnosis of M. tuberculosis infection in BCG vaccinated children aged five and younger

Publié en ligne: 23 avr. 2010

Pages: 257 - 263

DOI: https://doi.org/10.2478/v10046-010-0001-1

Mots clés
Mantoux test, T-SPOT. test, tuberculosis, children

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