<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Free negatively charged carboxyl groups were selectively modified (neutralised) in sturgeon (Acipenser güldenstädti Br.) gonadotropic hormone (GTH) α and β subunits. Eleven free carboxyl groups, three in the α and eight in the β subunit, were neutralised by reaction with glycine ethyl ester. Investigation of reassociated α-β dimers (recombinants) comprising one or both modified subunits showed that specific hormonal activity was completely lost while immunoreactivity was lowered in comparison with that of the standard α-β dimer. CD-spectroscopy of the modified subunits did not indicate any considerable changes in their spatial structure. A conclusion was made that free COOH groups of GTH were important as bearers of the negative charge necessary for the hormone activity on the level of the hormone-specific membrane receptors.</p></abstract>

Free negatively charged carboxyl groups were selectively modified (neutralised) in sturgeon (Acipenser güldenstädti Br.) gonadotropic hormone (GTH) α and β subunits. Eleven free carboxyl groups, three in the α and eight in the β subunit, were neutralised by reaction with glycine ethyl ester. Investigation of reassociated α-β dimers (recombinants) comprising one or both modified subunits showed that specific hormonal activity was completely lost while immunoreactivity was lowered in comparison with that of the standard α-β dimer. CD-spectroscopy of the modified subunits did not indicate any considerable changes in their spatial structure. A conclusion was made that free COOH groups of GTH were important as bearers of the negative charge necessary for the hormone activity on the level of the hormone-specific membrane receptors.

Role of Carboxyl Groups in the Secondary Structure and Function of Sturgeon Gonadotropin

Institute of Biology, University of Latvia, Miera iela 3, Salaspils, LV-2169, LATVIA

Latvian Institute of Organic Synthesis, Aizkraukles iela 21, Rīga, LV-1006, LATVIA

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

Free negatively charged carboxyl groups were selectively modified (neutralised) in sturgeon (Acipenser güldenstädti Br.) gonadotropic hormone (GTH) α and β...

Role of Carboxyl Groups in the Secondary Structure and Function of Sturgeon Gonadotropin

Publié en ligne: 29 nov. 2008

Pages: 110 - 114

DOI: https://doi.org/10.2478/v10046-008-0014-1

Mots clés
fish gonadotropic hormone, subunits, chemical modification, secondary structure, biological function, CD-spectroscopy

This content is open access.

Role of Carboxyl Groups in the Secondary Structure and Function of Sturgeon Gonadotropin

Publié en ligne: 29 nov. 2008

Pages: 110 - 114

DOI: https://doi.org/10.2478/v10046-008-0014-1

Mots clésfish gonadotropic hormone, subunits, chemical modification, secondary structure, biological function, CD-spectroscopy

This content is open access.

Mots clés
fish gonadotropic hormone, subunits, chemical modification, secondary structure, biological function, CD-spectroscopy