<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The high sensitivity of Fanconi's anemia (FA) cells to drug induced DNA interstrand crosslinks (ICL) such as diepoxybutane (DEB) was used as a part of FA screening in the children with clinical suspicion of FA. The study considered a total of 66 children with the hematological and/or congenital phenotypic symptoms reminiscent of FA. Blood samples from patients with clinical suspicion of FA and controls were collected for chromosome fragility evaluation by the DEB test. According to the results of DEB test, the patients were divided into two subgroups: FA displaying typical DEB sensitive cellular response and non FA.</p><p>In this study, 10 out of 66 patients were found to have a FA cellular phenotype. The percentage of DEB-induced aberrant cells was increased more than 26 times in FA patients (range 22.00-82.00% with a mean of 48.32%) when compared to non FA patients (range 0.00-12.00% with a mean of 1.84%). The number of DEB-induced breaks/ cells was more than 68 times higher in FA patients (range 0.26-4.39 with a mean of 1.37 breaks/cell) when compared to non FA patients (range 0.00-0.20 with a mean of 0.02 breaks/cell). The spontaneous chromosome fragility values in FA patients were overlapping those in non FA patients. Our results indicate that the DEB sensitivity test is the most reliable <italic>in vitro</italic> method for verification of the FA cellular phenotype.</p></abstract>

The high sensitivity of Fanconi's anemia (FA) cells to drug induced DNA interstrand crosslinks (ICL) such as diepoxybutane (DEB) was used as a part of FA screening in the children with clinical suspicion of FA. The study considered a total of 66 children with the hematological and/or congenital phenotypic symptoms reminiscent of FA. Blood samples from patients with clinical suspicion of FA and controls were collected for chromosome fragility evaluation by the DEB test. According to the results of DEB test, the patients were divided into two subgroups: FA displaying typical DEB sensitive cellular response and non FA.In this study, 10 out of 66 patients were found to have a FA cellular phenotype. The percentage of DEB-induced aberrant cells was increased more than 26 times in FA patients (range 22.00-82.00% with a mean of 48.32%) when compared to non FA patients (range 0.00-12.00% with a mean of 1.84%). The number of DEB-induced breaks/ cells was more than 68 times higher in FA patients (range 0.26-4.39 with a mean of 1.37 breaks/cell) when compared to non FA patients (range 0.00-0.20 with a mean of 0.02 breaks/cell). The spontaneous chromosome fragility values in FA patients were overlapping those in non FA patients. Our results indicate that the DEB sensitivity test is the most reliable in vitro method for verification of the FA cellular phenotype.

Diagnosis of Fanconi's Anemia by Diepoxybutane Analysis in Children from Serbia

Department of Medical Genetics, Mother and Child Health Care Institute of Serbia "Dr. Vukan Cupic", Belgrade, Serbia

Chair of Genetics and Evolution, Faculty of Biology, University of Belgrade, Serbia

Chair of Pediatrics, Faculty of Medicine, University of Belgrade, Serbia

Department of Hematology-Oncology, Mother and Child Health Care Institute of Serbia "Dr. Vukan Cupic", Belgrade, Serbia

Department of Bone Marrow Transplantation with Laboratory for Cryobiology, Mother and Child Health Care Institute of Serbia "Dr. Vukan Cupic", Belgrade, Serbia

Department of Hematology-Oncology, Faculty of Medicine, University Children's Hospital, University of Belgrade, Serbia

Balkan Journal of Medical Genetics

{"article-title":"Diagnosis of Fanconi's Anemia by Diepoxybutane Analysis in Children from Serbia"}

The high sensitivity of Fanconi's anemia (FA) cells to drug induced DNA interstrand crosslinks (ICL) such as diepoxybutane (DEB) was used as a part of FA...

Diagnosis of Fanconi's Anemia by Diepoxybutane Analysis in Children from Serbia

Publié en ligne: 08 déc. 2011

Pages: 65 - 70

DOI: https://doi.org/10.2478/v10034-011-0048-6

Mots clés
Chromosome fragility, Diepoxybutane (DEB), Fanconi's anemia (FA)

This content is open access.

Diagnosis of Fanconi's Anemia by Diepoxybutane Analysis in Children from Serbia

Publié en ligne: 08 déc. 2011

Pages: 65 - 70

DOI: https://doi.org/10.2478/v10034-011-0048-6

Mots clésChromosome fragility, Diepoxybutane (DEB), Fanconi's anemia (FA)

This content is open access.

Mots clés
Chromosome fragility, Diepoxybutane (DEB), Fanconi's anemia (FA)