<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>The adsorption model for an accurate prediction of the analyte retention in the normal - phase liquid chromatography with a binary mobile phase has been proposed. This model was derived using a thermody-namically consistent modified competitive Langmuir isotherm. The performance of the proposed equation was compared with two retention models reported in literature. All models were verified for different NP-LC systems by use of three criteria: the sum of squared differences between the experimental and theoretical data, approximation of the standard deviation and the Fisher test.</p></abstract>

The adsorption model for an accurate prediction of the analyte retention in the normal - phase liquid chromatography with a binary mobile phase has been proposed. This model was derived using a thermody-namically consistent modified competitive Langmuir isotherm. The performance of the proposed equation was compared with two retention models reported in literature. All models were verified for different NP-LC systems by use of three criteria: the sum of squared differences between the experimental and theoretical data, approximation of the standard deviation and the Fisher test.

Modeling of the influence of the modifier concentration on the retention process in NP-HPLC

Department of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland

Polish Journal of Chemical Technology

The adsorption model for an accurate prediction of the analyte retention in the normal - phase liquid chromatography with a binary mobile phase has been...

Modeling of the influence of the modifier concentration on the retention process in NP-HPLC

Publié en ligne: 03 avr. 2008

Pages: 52 - 56

DOI: https://doi.org/10.2478/v10026-008-0013-7

Mots clés
NP-HPLC, retention models, mobile phase composition

This content is open access.

Modeling of the influence of the modifier concentration on the retention process in NP-HPLC

Publié en ligne: 03 avr. 2008

Pages: 52 - 56

DOI: https://doi.org/10.2478/v10026-008-0013-7

Mots clésNP-HPLC, retention models, mobile phase composition

This content is open access.

Mots clés
NP-HPLC, retention models, mobile phase composition