<abstract xmlns="http://www.w3.org/1999/xhtml">

The present study strives to assess the cardioprotective role of phosphocreatine as an agent for postconditioning and perconditioning of isolated rat heart.
Rat hearts (n=30) were perfused with a Langendorff apparatus and randomly assigned to three groups subjected to 20 minutes of global ischemia and 30 minutes of reperfusion: control group (untreated rat hearts), postconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of reperfusion), and perconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of ischemia). During the experimental protocol, cardiodynamic parameters were evaluated, while oxidative stress parameters such as superoxide anion radical, hydrogen peroxide, nitrites and index of lipid peroxidation were determined in coronary venous effluent.
Postconditioning and perconditioning with phosphocreatine improved contractile function, heart rate and coronary flow, while the examined oxidative stress parameters in coronary venous effluent were significantly reduced in groups of treated rat hearts. The results of this study indicate that phosphocreatine has the potential as a therapeutic agent for perconditioning and postconditioning the heart in ischemia reperfusion injury.
</abstract>

The present study strives to assess the cardioprotective role of phosphocreatine as an agent for postconditioning and perconditioning of isolated rat heart.
Rat hearts (n=30) were perfused with a Langendorff apparatus and randomly assigned to three groups subjected to 20 minutes of global ischemia and 30 minutes of reperfusion: control group (untreated rat hearts), postconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of reperfusion), and perconditioning group (hearts treated with 0.2 mmol/l of phosphocreatine during the first 5 minutes of ischemia). During the experimental protocol, cardiodynamic parameters were evaluated, while oxidative stress parameters such as superoxide anion radical, hydrogen peroxide, nitrites and index of lipid peroxidation were determined in coronary venous effluent.
Postconditioning and perconditioning with phosphocreatine improved contractile function, heart rate and coronary flow, while the examined oxidative stress parameters in coronary venous effluent were significantly reduced in groups of treated rat hearts. The results of this study indicate that phosphocreatine has the potential as a therapeutic agent for perconditioning and postconditioning the heart in ischemia reperfusion injury.

The Role of Phosphocreatine in the Perconditioning and Postconditioning of Isolated Rat Heart

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The present study strives to assess the cardioprotective role of phosphocreatine as an agent for postconditioning and perconditioning of isolated rat...

The Role of Phosphocreatine in the Perconditioning and Postconditioning of Isolated Rat Heart

Publié en ligne: 11 juil. 2022

Pages: 111 - 119

Reçu: 28 mai 2022

Accepté: 30 mai 2022

DOI: https://doi.org/10.2478/sjecr-2022-0021

Mots clés
Langendorff apparatus, creatin phosphate, cardioprotection, oxidative stress

© 2022 Justyna Świerczyńska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Role of Phosphocreatine in the Perconditioning and Postconditioning of Isolated Rat Heart

Publié en ligne: 11 juil. 2022

Pages: 111 - 119

Reçu: 28 mai 2022

Accepté: 30 mai 2022

DOI: https://doi.org/10.2478/sjecr-2022-0021

Mots clésLangendorff apparatus, creatin phosphate, cardioprotection, oxidative stress

© 2022 Justyna Świerczyńska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
Langendorff apparatus, creatin phosphate, cardioprotection, oxidative stress