<abstract xmlns="http://www.w3.org/1999/xhtml">The ability of physiological (1α,25-dihydroxyvitamin D3, retinoic acid) and non-physiological (various LPS) agents and their combinations to influence the direction of promonocytic THP-1 cell differentiation was studied.The differentiating activity of the agents was evaluated by the expression and the ratio of surface receptors (TLR4, CD11b, and CD14) as well as by the change in THP-1 cell phagocytic activity of different degree of differentiation by Flow cytometry.The THP-1 cell differentiation by VD3 was shown to lead probably to the formation of classical monocytes.Summarizing we can conclude that VD3 induces the THP-1 cells differentiation with the formation of classical monocytes and the sequence of 1α, 25-dihydroxyvitamin D3 and non-toxic LPS R. capsulatus PG causes the THP-1 cells differentiation with the formation of inflammatory or intermediate monocytes.</abstract>

The ability of physiological (1α,25-dihydroxyvitamin D3, retinoic acid) and non-physiological (various LPS) agents and their combinations to influence the direction of promonocytic THP-1 cell differentiation was studied.The differentiating activity of the agents was evaluated by the expression and the ratio of surface receptors (TLR4, CD11b, and CD14) as well as by the change in THP-1 cell phagocytic activity of different degree of differentiation by Flow cytometry.The THP-1 cell differentiation by VD3 was shown to lead probably to the formation of classical monocytes.Summarizing we can conclude that VD3 induces the THP-1 cells differentiation with the formation of classical monocytes and the sequence of 1α, 25-dihydroxyvitamin D3 and non-toxic LPS R. capsulatus PG causes the THP-1 cells differentiation with the formation of inflammatory or intermediate monocytes.

Effect of Various Agents on the Direction of THP-1 Cell Differentiation

FSBIS Institute of Basic Biological Problems Russian Academy of Sciences

Federal State Autonomous Educational Institution of High Professional Education

I.M. Sechenov’s First Moscow State Medical University of Russian Healthcare Ministry

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Effect of Various Agents on the Direction of THP-1 Cell Differentiation"}

The ability of physiological (1α,25-dihydroxyvitamin D3, retinoic acid) and non-physiological (various LPS) agents and their combinations to influence the...

Effect of Various Agents on the Direction of THP-1 Cell Differentiation

Publié en ligne: 29 oct. 2018

Pages: 263 - 269

Reçu: 10 mars 2018

Accepté: 09 avr. 2018

DOI: https://doi.org/10.2478/sjecr-2018-0029

Mots clés
ТНР-1 cells, vitamin D3, retinoic acid, lipopolysaccharides, receptor expression, phagocytosis

© 2018 Svetlana V. Zubova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of Various Agents on the Direction of THP-1 Cell Differentiation

Publié en ligne: 29 oct. 2018

Pages: 263 - 269

Reçu: 10 mars 2018

Accepté: 09 avr. 2018

DOI: https://doi.org/10.2478/sjecr-2018-0029

Mots clésТНР-1 cells, vitamin D3, retinoic acid, lipopolysaccharides, receptor expression, phagocytosis

© 2018 Svetlana V. Zubova et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
ТНР-1 cells, vitamin D3, retinoic acid, lipopolysaccharides, receptor expression, phagocytosis