<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Twenty polymorphic microsatellite markers were developed, using Next Generation Sequencing (Illumina), from genomic DNA of Parashorea malaanonan, a species of the Dipterocarpa­ceae which is ecologically and economically important in the Philippines. Thirty adult trees from a natural population were used to assess the success of PCR amplification and the degree of polymorphism. The number of alleles per locus varied from three to 13, and observed and expected heterozygosity varied from 0.200 to 0.808 and from 0.301 to 0.890 respectively. Total exclusion probabilities for the first and second parents over the 20 loci were 0.99932499 and 0.99999723 respectively. The high level of polymorphism at these loci makes it possible to obtain precise estimates of genetic parameters and thus the markers will help in studies on population genetics, conservation gene­tics, and molecular ecology of P. malaanonan.</p></abstract>

Twenty polymorphic microsatellite markers were developed, using Next Generation Sequencing (Illumina), from genomic DNA of Parashorea malaanonan, a species of the Dipterocarpa­ceae which is ecologically and economically important in the Philippines. Thirty adult trees from a natural population were used to assess the success of PCR amplification and the degree of polymorphism. The number of alleles per locus varied from three to 13, and observed and expected heterozygosity varied from 0.200 to 0.808 and from 0.301 to 0.890 respectively. Total exclusion probabilities for the first and second parents over the 20 loci were 0.99932499 and 0.99999723 respectively. The high level of polymorphism at these loci makes it possible to obtain precise estimates of genetic parameters and thus the markers will help in studies on population genetics, conservation gene­tics, and molecular ecology of P. malaanonan.

Development of microsatellite markers from genomic DNA of Parashorea malaanonan (Dipterocarpaceae) using next-generation sequencing

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Twenty polymorphic microsatellite markers were developed, using Next Generation Sequencing (Illumina), from genomic DNA of Parashorea malaanonan, a species of...

Development of microsatellite markers from genomic DNA of Parashorea malaanonan (Dipterocarpaceae) using next-generation sequencing

Publié en ligne: 28 févr. 2019

Pages: 22 - 25

DOI: https://doi.org/10.2478/sg-2019-0004

Mots clés
Dipterocarpaceae, microsatellite markers, Parashorea malaanonan, next generation sequencing, simple sequence repeat

© 2019 Crusty E. Tinio, et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Development of microsatellite markers from genomic DNA of Parashorea malaanonan (Dipterocarpaceae) using next-generation sequencing

Publié en ligne: 28 févr. 2019

Pages: 22 - 25

DOI: https://doi.org/10.2478/sg-2019-0004

Mots clésDipterocarpaceae, microsatellite markers, Paras­horea malaanonan, next generation sequencing, simple sequence repeat

© 2019 Crusty E. Tinio, et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Dipterocarpaceae, microsatellite markers, Parashorea malaanonan, next generation sequencing, simple sequence repeat