<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Studies were conducted to characterize morphological and molecular profiles of two isolates of Paratrichodorus porosus (SZ1 and SZ2) which were recovered from Acacia mangium in Tianxinshan and Gleichenia linearis in Yangmeikeng environmental monitoring sites in Shenzhen, China, respectively. Analysis of morphometric, morphological and molecular characters revealed these two Shenzhen isolates are identical to P. porosus. Measurements of both study isolates lie within the ranges for P. porosus. It is typologically characterized by possessing a clearly swollen body cuticle after fixation, an onchiostyle ventrally curved, 46–58 μm long, a pharyngeal bulb usually with a well developed anterior-dorsal intestinal overlap, a secretoryexcretory pore opening between the nerve ring and anterior end of pharyngeal bulb, 90–110 μm from the anterior end, a reproductive system with didelphic, amphidelphic, without spermathecae, a pore-like vulva in ventral view and occupying 52.0 %–59.5 % of total body length from anterior end, a short and barrel-shaped vagina with small sclerotizations, a pair of ventromedian advulvar body pores located prevulvar and postvulvar, a rounded tail and a subterminal anus in females. The sequence analysis based on partial rDNA 18S gene and 28S D2/D3 expansion segment confirm its identity as P. porosus. This is the first report of P. porosus associated with A. mangium and G. linearis.</p></abstract>

Studies were conducted to characterize morphological and molecular profiles of two isolates of Paratrichodorus porosus (SZ1 and SZ2) which were recovered from Acacia mangium in Tianxinshan and Gleichenia linearis in Yangmeikeng environmental monitoring sites in Shenzhen, China, respectively. Analysis of morphometric, morphological and molecular characters revealed these two Shenzhen isolates are identical to P. porosus. Measurements of both study isolates lie within the ranges for P. porosus. It is typologically characterized by possessing a clearly swollen body cuticle after fixation, an onchiostyle ventrally curved, 46–58 μm long, a pharyngeal bulb usually with a well developed anterior-dorsal intestinal overlap, a secretoryexcretory pore opening between the nerve ring and anterior end of pharyngeal bulb, 90–110 μm from the anterior end, a reproductive system with didelphic, amphidelphic, without spermathecae, a pore-like vulva in ventral view and occupying 52.0 %–59.5 % of total body length from anterior end, a short and barrel-shaped vagina with small sclerotizations, a pair of ventromedian advulvar body pores located prevulvar and postvulvar, a rounded tail and a subterminal anus in females. The sequence analysis based on partial rDNA 18S gene and 28S D2/D3 expansion segment confirm its identity as P. porosus. This is the first report of P. porosus associated with A. mangium and G. linearis.

Morphological and molecular characterization of two isolates of Paratrichodorus porosus from Shenzhen, China

Department of Plant Protection, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, People’s Republic of China

Department of Plant Pathology, North Carolina State University, Raleigh, NC, 27695-7613, USA

Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC, 27607, USA

Shenzhen Environmental Monitoring Center, Shenzhen, 518049, People’s Republic of China

College of Life Science, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, People’s Republic of China

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Studies were conducted to characterize morphological and molecular profiles of two isolates of Paratrichodorus porosus (SZ1 and SZ2) which were recovered from...

Morphological and molecular characterization of two isolates of Paratrichodorus porosus from Shenzhen, China

Publié en ligne: 04 nov. 2014

Pages: 323 - 330

DOI: https://doi.org/10.2478/s11687-014-0248-1

Mots clés
Paratrichodorus porosus, Acacia mangium, Gleichenia linearis, 18S small subunit rDNA, 28S large subunit rDNA, PCR, stubby-root nematode, taxonomy

© 2014 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological and molecular characterization of two isolates of Paratrichodorus porosus from Shenzhen, China

Publié en ligne: 04 nov. 2014

Pages: 323 - 330

DOI: https://doi.org/10.2478/s11687-014-0248-1

Mots clésParatrichodorus porosus, Acacia mangium, Gleichenia linearis, 18S small subunit rDNA, 28S large subunit rDNA, PCR, stubby-root nematode, taxonomy

© 2014 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Paratrichodorus porosus, Acacia mangium, Gleichenia linearis, 18S small subunit rDNA, 28S large subunit rDNA, PCR, stubby-root nematode, taxonomy