<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Additional data on the occurrence and distribution of Xiphinema non-americanum group species in Romania are provided. Xiphinema diversicaudatum, X. index, X. vuittenezi and X. italiae were recovered from vineyards and cherry fruit trees; adults and juvenile stages were described and analysed and the morphology/variability discussed. Multiplex PCR diagnostic test using species-specific primers designed by Wang et al. (2003) yielded amplification products with expected lengths for all screened populations of these four species. Two ribosomal markers (D2-D3 28 LSU rDNA and ITS) were sequenced and ITS RFLP patterns were obtained from two X. vuittenezi populations, which have shown some morphological differences. Comparatively low level of interpopulation genetic dissimilarity (&lt;1 %) was revealed for both markers (for D2D3 — 0.5 %; for ITS — 0.7 %). Both populations of X. vuittenezi studied produced identical ITS-RFLP specific pattern that clearly identify this species.</p></abstract>

Additional data on the occurrence and distribution of Xiphinema non-americanum group species in Romania are provided. Xiphinema diversicaudatum, X. index, X. vuittenezi and X. italiae were recovered from vineyards and cherry fruit trees; adults and juvenile stages were described and analysed and the morphology/variability discussed. Multiplex PCR diagnostic test using species-specific primers designed by Wang et al. (2003) yielded amplification products with expected lengths for all screened populations of these four species. Two ribosomal markers (D2-D3 28 LSU rDNA and ITS) were sequenced and ITS RFLP patterns were obtained from two X. vuittenezi populations, which have shown some morphological differences. Comparatively low level of interpopulation genetic dissimilarity (<1 %) was revealed for both markers (for D2D3 — 0.5 %; for ITS — 0.7 %). Both populations of X. vuittenezi studied produced identical ITS-RFLP specific pattern that clearly identify this species.

Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR

University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Blvd. Mărăşti, Bucharest, 011464, România

Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113, Sofia, Bulgaria

Central Phytosanitary Laboratory, 11 Voluntari Blv., 077190, Voluntari, Ilfov, România

Istituto per la Protezione delle Piante (IPP), Sezione di Bari, Consiglio Nazionale delle Ricerche, (C.N.R.), Via G. Amendola 122/D, 70126, Bari, Italy

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Additional data on the occurrence and distribution of Xiphinema non-americanum group species in Romania are provided. Xiphinema diversicaudatum, X. index, X....

Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR

Publié en ligne: 22 févr. 2014

Pages: 215 - 231

DOI: https://doi.org/10.2478/s11687-013-0133-3

Mots clés
distribution, juvenile stages, D2-D3, ITS, RFLP

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Morphological characterisation and diagnostics of Xiphinema non-americanum group species (Nematoda: Longidoridae) from Romania using mutiplex PCR

Publié en ligne: 22 févr. 2014

Pages: 215 - 231

DOI: https://doi.org/10.2478/s11687-013-0133-3

Mots clésdistribution, juvenile stages, D2-D3, ITS, RFLP

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
distribution, juvenile stages, D2-D3, ITS, RFLP