<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>Reinvestigation of vitellogenesis in the caryophyllidean cestode Caryophyllaeus laticeps (Pallas, 1781) has been performed using light microscope (LM) and transmission electron microscopy (TEM), and cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP) for glycogen. Vitellogenesis is generally similar to that reported in the past, however, some new observations were made. The present study reveals the first evidence of: (i) interstitial tissue in the vitelline follicles, (ii) lipid droplets in maturing and mature vitellocytes from vitelline follicles, and (iii) lamellar bodies in vitellocytes from the vitelloduct in C. laticeps. Projections of interstitial tissue surround each vitellocyte and the follicle periphery. The perinuclear cytoplasm of the interstitial tissue contains granular endoplasmic reticulum and vesicles of various size and density. Cytoplasmic osmiophobic lipid droplets and lamellar bodies, previously believed to be absent in most caryophyllid cestodes, are readily apparent in vitellocytes of C. laticeps. The origin and presumed function of these inclusions are discussed. On the other hand, the formation and storage of massive amounts of glycogen in the nucleus and large amounts in the cytoplasm of mature vitelline cells are similar to the condition found in other caryophyllids. Results are compared and contrasted with previous studies on vitellogenesis in other monopleuroid cestodes (Amphilinidea and Gyrocotylidea) as well as polypleuroid cestodes (Spathebothriidea) and the remaining strobilated Eucestoda.</p></abstract>

Reinvestigation of vitellogenesis in the caryophyllidean cestode Caryophyllaeus laticeps (Pallas, 1781) has been performed using light microscope (LM) and transmission electron microscopy (TEM), and cytochemical staining with periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP) for glycogen. Vitellogenesis is generally similar to that reported in the past, however, some new observations were made. The present study reveals the first evidence of: (i) interstitial tissue in the vitelline follicles, (ii) lipid droplets in maturing and mature vitellocytes from vitelline follicles, and (iii) lamellar bodies in vitellocytes from the vitelloduct in C. laticeps. Projections of interstitial tissue surround each vitellocyte and the follicle periphery. The perinuclear cytoplasm of the interstitial tissue contains granular endoplasmic reticulum and vesicles of various size and density. Cytoplasmic osmiophobic lipid droplets and lamellar bodies, previously believed to be absent in most caryophyllid cestodes, are readily apparent in vitellocytes of C. laticeps. The origin and presumed function of these inclusions are discussed. On the other hand, the formation and storage of massive amounts of glycogen in the nucleus and large amounts in the cytoplasm of mature vitelline cells are similar to the condition found in other caryophyllids. Results are compared and contrasted with previous studies on vitellogenesis in other monopleuroid cestodes (Amphilinidea and Gyrocotylidea) as well as polypleuroid cestodes (Spathebothriidea) and the remaining strobilated Eucestoda.

Reinvestigation of vitellogenesis in Caryophyllaeus laticeps (Pallas, 1781) (Cestoda, Caryophyllidea, Caryophyllaeidae), monozoic tapeworm of Abramis brama (Pisces, Teleostei)

Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic

Faculty of Biological Sciences, University of South Bohemia, 370 05, České Budějovice, Czech Republic

Department of Biological Sciences, University at Albany, State University of New York, Albany, New York, 12222, USA

Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Branišovská 31, 370 05, České Budějovice, Czech Republic

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Reinvestigation of vitellogenesis in the caryophyllidean cestode Caryophyllaeus laticeps (Pallas, 1781) has been performed using light microscope (LM) and...

Reinvestigation of vitellogenesis in Caryophyllaeus laticeps (Pallas, 1781) (Cestoda, Caryophyllidea, Caryophyllaeidae), monozoic tapeworm of Abramis brama (Pisces, Teleostei)

Publié en ligne: 27 avr. 2013

Pages: 73 - 81

DOI: https://doi.org/10.2478/s11687-013-0110-x

Mots clés
Caryophyllidea, Caryophyllaeus laticeps, vitellogenesis; vitellocyte, ultrastructure

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Reinvestigation of vitellogenesis in Caryophyllaeus laticeps (Pallas, 1781) (Cestoda, Caryophyllidea, Caryophyllaeidae), monozoic tapeworm of Abramis brama (Pisces, Teleostei)

Publié en ligne: 27 avr. 2013

Pages: 73 - 81

DOI: https://doi.org/10.2478/s11687-013-0110-x

Mots clésCaryophyllidea, Caryophyllaeus laticeps, vitellogenesis; vitellocyte, ultrastructure

© 2013 Institute of Parasitology, SAS, Košice, Slovakia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Caryophyllidea, Caryophyllaeus laticeps, vitellogenesis; vitellocyte, ultrastructure