<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of <italic>Coptotermes curvignathus</italic> in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as <italic>Bacillus</italic> sp., <italic>Lysinibacillus</italic> sp. and <italic>Acinetobacter</italic> sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. <italic>Lysinibacillus</italic> sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of <italic>Bacillus</italic> sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis.</p></abstract>

Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification require high inputs of energy and chemicals, resulting in the release of highly toxic effluents. The ability of gut flora of Coptotermes curvignathus in lignin degradation was investigated in this study. Production of ligninolytic enzymes was done in an aerated submerged fermentation system with kraft lignin as sole carbon source. The degradation experiment was carried out for 7 days at 30 °C, pH 7. Three potential lignin degraders identified as Bacillus sp., Lysinibacillus sp. and Acinetobacter sp. were successfully isolated. The bacterial growth and secretion of extracellular ligninolytic enzymes confirmed metabolism of kraft lignin by the identified strains. Lysinibacillus sp., a novel lignin degrader showed highest manganese peroxidase (76.36 ± 15.74 U/L) and laccase activity (70.67 ± 16.82 U/L) after 7 and 6 days of incubation respectively, while maximal activity of lignin peroxidase (262.49 ± 0.92 U/L) was recorded after 7 days in culture supernatants of Bacillus sp. With respect to the activity of the secreted enzymes, the lignin degrading potential of these bacterial strains can be explored in the valorisations of lignocellulosic biomass in industrial processes such as pulping, bioethanol production, fine chemicals and materials synthesis.

Production of Ligninolytic Enzymes by <italic>Coptotermes curvignathus</italic> Gut Bacteria

Department of Chemical Engineering Technology, Faculty of Engineering Technology

Department of Crop Science, Faculty of Agriculture and Food Sciences

Environmental and Climate Technologies

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Production of Ligninolytic Enzymes by  Gut Bacteria

Maximum utilization of lignocellulosic biomass is contingent upon degrading the recalcitrant lignin polymer. Conventional methods employed in delignification...

Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria

Publié en ligne: 19 juil. 2019

Pages: 111 - 121

DOI: https://doi.org/10.2478/rtuect-2019-0008

Mots clés
Enzymes, kraft lignin, laccase, peroxidases, termites

© 2019 Fadilah Ayeronfe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Production of Ligninolytic Enzymes by Coptotermes curvignathus Gut Bacteria

Publié en ligne: 19 juil. 2019

Pages: 111 - 121

DOI: https://doi.org/10.2478/rtuect-2019-0008

Mots clésEnzymes, kraft lignin, laccase, peroxidases, termites

© 2019 Fadilah Ayeronfe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Enzymes, kraft lignin, laccase, peroxidases, termites