<abstract xmlns="http://www.w3.org/1999/xhtml">

<bold>Background</bold>: Sepsis is the leading cause of morbidity and mortality in intensive care units. This study explored the possible role of vascular endothelial growth factor-C (VEGF-C) and podoplanin (PDPN) in sepsis.
<bold>Methods</bold>: 22 Wistar rats were divided into three groups: two experimental (Group A and B, n=8/8) and a control (Group C, n=6). Sepsis was induced with intraperitoneal injection of ESBL (extended-spectrum beta-lactamases)-producing E-coli live bacteria for group A and with lipopolysaccharide for group B. Sterile saline solution was injected for group C. Blood samples were collected after 24 hours to determine the serum level of VEGF-C, and PDPN expression was examined in liver, kidney, and lung tissues. Bacteremia was assessed for group A.
<bold>Results</bold>: Higher serum levels of VEGF-C were found in Group A vs C (p=0.05) and group B vs. C (p=0.004), respectively.VEGF-C was also increased in animals with negative- vs. positive blood cultures from group A (p=0.04) and from group B vs. those with positive blood cultures from group A (p=0.03). High intensity of PDPN tissue expression was observed in the pulmonary alveolocytes from Group A and epithelium of the proximal renal tubules in groups B and C, compared to group A.
<bold>Conclusions</bold>: Circulating VEGF-C can be succesfuly used as a biomarker of sepsis with negative blood cultures and high risk of renal failure, whereas PDPN seems to exert a protective role against lung injuries in live bacteria-induced sepsis.
</abstract>

Background: Sepsis is the leading cause of morbidity and mortality in intensive care units. This study explored the possible role of vascular endothelial growth factor-C (VEGF-C) and podoplanin (PDPN) in sepsis.
Methods: 22 Wistar rats were divided into three groups: two experimental (Group A and B, n=8/8) and a control (Group C, n=6). Sepsis was induced with intraperitoneal injection of ESBL (extended-spectrum beta-lactamases)-producing E-coli live bacteria for group A and with lipopolysaccharide for group B. Sterile saline solution was injected for group C. Blood samples were collected after 24 hours to determine the serum level of VEGF-C, and PDPN expression was examined in liver, kidney, and lung tissues. Bacteremia was assessed for group A.
Results: Higher serum levels of VEGF-C were found in Group A vs C (p=0.05) and group B vs. C (p=0.004), respectively.VEGF-C was also increased in animals with negative- vs. positive blood cultures from group A (p=0.04) and from group B vs. those with positive blood cultures from group A (p=0.03). High intensity of PDPN tissue expression was observed in the pulmonary alveolocytes from Group A and epithelium of the proximal renal tubules in groups B and C, compared to group A.
Conclusions: Circulating VEGF-C can be succesfuly used as a biomarker of sepsis with negative blood cultures and high risk of renal failure, whereas PDPN seems to exert a protective role against lung injuries in live bacteria-induced sepsis.

VEGF-C and podoplanin, as biomarkers of sepsis. An experimental study

George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Pathophysiology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Pathology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Microbiology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Humoral Immunology Laboratory Center for Advanced Medical and Pharmaceutical Research

Anesthesiology and Intensive Care Clinic, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"VEGF-C and podoplanin, as biomarkers of sepsis. An experimental study"}

Background: Sepsis is the leading cause of morbidity and mortality in intensive care units. This study explored the possible role of vascular endothelial...

VEGF-C and podoplanin, as biomarkers of sepsis. An experimental study

Publié en ligne: 22 oct. 2021

Pages: 403 - 412

Reçu: 01 sept. 2021

Accepté: 27 sept. 2021

DOI: https://doi.org/10.2478/rrlm-2021-0030

Mots clés
podoplanin, vascular endothelial growth factor, sepsis, lipopolysaccharide

© 2021 Emoke Almasy et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

VEGF-C and podoplanin, as biomarkers of sepsis. An experimental study

Publié en ligne: 22 oct. 2021

Pages: 403 - 412

Reçu: 01 sept. 2021

Accepté: 27 sept. 2021

DOI: https://doi.org/10.2478/rrlm-2021-0030

Mots cléspodoplanin, vascular endothelial growth factor, sepsis, lipopolysaccharide

© 2021 Emoke Almasy et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
podoplanin, vascular endothelial growth factor, sepsis, lipopolysaccharide