<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Meningiomas are classified by the World Health Organization (WHO) in three grades, based on morphological features. Independent of this grading, the presence of calcification in meningiomas influences their growth rate. The messenger RNA of matrix Gla protein (MGP), an extra-hepatic protein with different conformations involved in the homeostasis of ectopic calcification has been found in meningiomas and was shown to be regulated in breast cancer by miR-155-5p, a specific micro RNA. Therefore, we investigated the expression of miR-155-5p and its relationship with local MGP conformations in different grade meningiomas. According to the WHO classification, our 41 samples of meningiomas were stratified in groups WHO I and WHO II. Using real time polymerase chain reaction, we observed a higher miR-155-5p expression in group WHO I versus group WHO II [with a fold change (FC) of 3.83, p=0.027)]. Moreover, the expression of miR-155-5p was higher in calcified tumors compared to non-calcified tumors in all samples (FC=3.01, p=0.047) and in group WHO I (FC=3.65, p=0.048). Utilizing immunohistochemistry, we determined the concurrent presence of all MGP conformations in calcified meningiomas. This study was the first to establish higher miR-155-5p expression in grade WHO I and calcified meningiomas, which could improve molecular classification and targeted therapy and also the presence of all MGP conformations in calcified meningiomas, confirming the existence of an anti-calcification mechanism in meningiomas..</p>
</abstract>

Meningiomas are classified by the World Health Organization (WHO) in three grades, based on morphological features. Independent of this grading, the presence of calcification in meningiomas influences their growth rate. The messenger RNA of matrix Gla protein (MGP), an extra-hepatic protein with different conformations involved in the homeostasis of ectopic calcification has been found in meningiomas and was shown to be regulated in breast cancer by miR-155-5p, a specific micro RNA. Therefore, we investigated the expression of miR-155-5p and its relationship with local MGP conformations in different grade meningiomas. According to the WHO classification, our 41 samples of meningiomas were stratified in groups WHO I and WHO II. Using real time polymerase chain reaction, we observed a higher miR-155-5p expression in group WHO I versus group WHO II [with a fold change (FC) of 3.83, p=0.027)]. Moreover, the expression of miR-155-5p was higher in calcified tumors compared to non-calcified tumors in all samples (FC=3.01, p=0.047) and in group WHO I (FC=3.65, p=0.048). Utilizing immunohistochemistry, we determined the concurrent presence of all MGP conformations in calcified meningiomas. This study was the first to establish higher miR-155-5p expression in grade WHO I and calcified meningiomas, which could improve molecular classification and targeted therapy and also the presence of all MGP conformations in calcified meningiomas, confirming the existence of an anti-calcification mechanism in meningiomas..

The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas

“Iuliu Hațieganu” University of Medicine and Pharmacy

Department of Biochemistry and Pharmacology

“Victor Babeş” University of Medicine and Pharmacy

University of Medicine and Pharmacy of Craiova

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas"}

Meningiomas are classified by the World Health Organization (WHO) in three grades, based on morphological features. Independent of this grading, the presence...

The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas

Publié en ligne: 31 juil. 2021

Pages: 299 - 306

Reçu: 25 nov. 2020

Accepté: 11 juin 2021

DOI: https://doi.org/10.2478/rrlm-2021-0020

Mots clés
histology, biochemistry, molecular biology, experimental laboratory and clinical studies

© 2021 Simona Roxana Gheorghe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Expression of miR-155-5p and Local Matrix Gla Protein in Meningiomas

Publié en ligne: 31 juil. 2021

Pages: 299 - 306

Reçu: 25 nov. 2020

Accepté: 11 juin 2021

DOI: https://doi.org/10.2478/rrlm-2021-0020

Mots cléshistology, biochemistry, molecular biology, experimental laboratory and clinical studies

© 2021 Simona Roxana Gheorghe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
histology, biochemistry, molecular biology, experimental laboratory and clinical studies