<abstract xmlns="http://www.w3.org/1999/xhtml"><p><bold>Background/aim</bold>: This prospective study aimed to determine the presence of the most common carbapenemase genes, blaOXA-48, blaKPC, blaIMP, blaVIM and blaNDM on carbapenem resistant clinical K.pneumoniae and E.coli isolates. <bold>Materials and methods</bold>: Isolates were selected according to EUCAST guideline; gradient test and disc diffusion with both meropenem and ertapenem discs. Resistance rates of these isolates to other antimicrobial agents were also examined by disc diffusion method. Carbapenem resistance gene were investigated by using Real-Time PCR. <bold>Results</bold>: A total of 3845 E. coli and 1689 K.pneumoniae isolates from clinical samples between January 2015 and April 2017 were evaluated. The 419 isolates were found as carbapenem resistant but only the first resistant isolate (n=155; 126 K.pneumoniae and 29 E.coli) of each patient were included. Carbapenem resistant isolates were most frequently isolated from intensive care units (48.8%). Colistin was the most effective antibiotic (91.0%). The 121 (78.1%) of the tested isolates were positive for OXA-48 (103 K.pneumoniae and 18 E.coli) and 9 K. pneumoniae carrying blaNDM were also positive for blaOXA-48. VIM, IMP and KPC type carbapenemases were not detected in any isolates. <bold>Conclusion</bold>: Carbapenem-resistant pathogens have been shown to be able to develop resistance mechanisms with more than one carbapenemase encoding gene.</p></abstract>

Background/aim: This prospective study aimed to determine the presence of the most common carbapenemase genes, blaOXA-48, blaKPC, blaIMP, blaVIM and blaNDM on carbapenem resistant clinical K.pneumoniae and E.coli isolates. Materials and methods: Isolates were selected according to EUCAST guideline; gradient test and disc diffusion with both meropenem and ertapenem discs. Resistance rates of these isolates to other antimicrobial agents were also examined by disc diffusion method. Carbapenem resistance gene were investigated by using Real-Time PCR. Results: A total of 3845 E. coli and 1689 K.pneumoniae isolates from clinical samples between January 2015 and April 2017 were evaluated. The 419 isolates were found as carbapenem resistant but only the first resistant isolate (n=155; 126 K.pneumoniae and 29 E.coli) of each patient were included. Carbapenem resistant isolates were most frequently isolated from intensive care units (48.8%). Colistin was the most effective antibiotic (91.0%). The 121 (78.1%) of the tested isolates were positive for OXA-48 (103 K.pneumoniae and 18 E.coli) and 9 K. pneumoniae carrying blaNDM were also positive for blaOXA-48. VIM, IMP and KPC type carbapenemases were not detected in any isolates. Conclusion: Carbapenem-resistant pathogens have been shown to be able to develop resistance mechanisms with more than one carbapenemase encoding gene.

Arising Prevalence of OXA-48 producer <italic>Escherichia coli</italic> and OXA-48 with NDM co-producer <italic>Klebsiella pneumoniae</italic> Strains

Gulhane Faculty of Medicine, Department of Medical Microbiology

Faculty of Medicine, Department of Medical Microbiology

Istanbul Medical Faculty, Department of Medical Microbiology

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Arising Prevalence of OXA-48 producer  and OXA-48 with NDM co-producer  Strains

Background/aim: This prospective study aimed to determine the presence of the most common carbapenemase genes, blaOXA-48, blaKPC, blaIMP, blaVIM and blaNDM on...

Arising Prevalence of OXA-48 producer Escherichia coli and OXA-48 with NDM co-producer Klebsiella pneumoniae Strains

Publié en ligne: 30 juil. 2019

Pages: 319 - 326

Reçu: 25 janv. 2019

Accepté: 13 juin 2019

DOI: https://doi.org/10.2478/rrlm-2019-0030

Mots clés
Klebsiella pneumoniae, Escherichia coli, carbapenem resistance, antimicrobial resistance, Enterobacterales

© 2019 Aylin Uskudar-Guclu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Arising Prevalence of OXA-48 producer Escherichia coli and OXA-48 with NDM co-producer Klebsiella pneumoniae Strains

Publié en ligne: 30 juil. 2019

Pages: 319 - 326

Reçu: 25 janv. 2019

Accepté: 13 juin 2019

DOI: https://doi.org/10.2478/rrlm-2019-0030

Mots clésKlebsiella pneumoniae, Escherichia coli, carbapenem resistance, antimicrobial resistance, Enterobacterales

© 2019 Aylin Uskudar-Guclu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Klebsiella pneumoniae, Escherichia coli, carbapenem resistance, antimicrobial resistance, Enterobacterales