<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Background: Genetic variations, such as those affecting DNA repair genes, could represent susceptibility factors for sporadic colorectal cancer (CRC) as a result of their interaction with environmental factors. Materials and </p><p>methods: 80 female and 70 males patients diagnosed with sporadic CRC in the Surgical Clinic III Cluj were genotyped for Arg399Gln-XRCC1, Lys751Gln-XPD and Met241Thr-XRCC3 using PCR-RFLP methods. We also genotyped 100 females and 62 males, who formed the control group. Genotyping results were related to environmental risk factors, smoking habit and diet. Results: Male patients carriers of the Arg399Gln, Lys751Gln, Met241Thr had a 4.09 (95%CI[0.96-19.98],p=0.05)-fold, 5.95(95%CI[1.08-43.22],p=0.03)-fold and 3.73(95%CI[0.86- 18.53],p=0.05)- fold significantly increased risk to develop sporadic CRC if they smoked. A significantly increased risk for CRC was observed in females and males with high daily fried red meat intake, carriers of the Arg399Gln (OR 2.77 95%CI [1.34-6.82],p=0.015 and OR 8.64 95%CI[2.67-29.14],p&lt;0.001), Lys751Gln (OR 4.12 95%CI[1.37-12.74],p=0.007 and OR 5.06 95%CI[1.4-19.02],p=0.006), Met241Thr (OR5.92 95%CI[2.21- 16.23],p&lt;0.001 and OR 5.64 95%CI[1.52-21.7],p=0.022). Female patients with high fried red meat intake had a significantly higher risk to develop early-onset sporadic CRC if they were carriers of the Arg399Gln-XRCC1 (OR 5.14 95%CI[0.99-28.3],p=0.047), Thr241Met-XRCC3 (OR 6.67 95%CI[1.05-46.67],p=0.025) and Lys- 751Gln-XPD (OR 4.7 95%CI[0.99-23.32],p=0.034). Conclusions: In Romanians, the association between the mutated genotypes and environmental risk factors modulates the risk for sporadic CRC. Smoking in association with the Arg399Gln-XRCC1 genetic variation influences the early onset of sporadic colorectal cancer in females. Diet rich in fried red meat intake associated with Arg399Gln-XRCC1, Lys751Gln-XPD and Thr241Met- XRCC3 genetic variations significantly influences the early onset of sporadic colorectal cancer in females.</p></abstract>

Background: Genetic variations, such as those affecting DNA repair genes, could represent susceptibility factors for sporadic colorectal cancer (CRC) as a result of their interaction with environmental factors. Materials and methods: 80 female and 70 males patients diagnosed with sporadic CRC in the Surgical Clinic III Cluj were genotyped for Arg399Gln-XRCC1, Lys751Gln-XPD and Met241Thr-XRCC3 using PCR-RFLP methods. We also genotyped 100 females and 62 males, who formed the control group. Genotyping results were related to environmental risk factors, smoking habit and diet. Results: Male patients carriers of the Arg399Gln, Lys751Gln, Met241Thr had a 4.09 (95%CI[0.96-19.98],p=0.05)-fold, 5.95(95%CI[1.08-43.22],p=0.03)-fold and 3.73(95%CI[0.86- 18.53],p=0.05)- fold significantly increased risk to develop sporadic CRC if they smoked. A significantly increased risk for CRC was observed in females and males with high daily fried red meat intake, carriers of the Arg399Gln (OR 2.77 95%CI [1.34-6.82],p=0.015 and OR 8.64 95%CI[2.67-29.14],p<0.001), Lys751Gln (OR 4.12 95%CI[1.37-12.74],p=0.007 and OR 5.06 95%CI[1.4-19.02],p=0.006), Met241Thr (OR5.92 95%CI[2.21- 16.23],p<0.001 and OR 5.64 95%CI[1.52-21.7],p=0.022). Female patients with high fried red meat intake had a significantly higher risk to develop early-onset sporadic CRC if they were carriers of the Arg399Gln-XRCC1 (OR 5.14 95%CI[0.99-28.3],p=0.047), Thr241Met-XRCC3 (OR 6.67 95%CI[1.05-46.67],p=0.025) and Lys- 751Gln-XPD (OR 4.7 95%CI[0.99-23.32],p=0.034). Conclusions: In Romanians, the association between the mutated genotypes and environmental risk factors modulates the risk for sporadic CRC. Smoking in association with the Arg399Gln-XRCC1 genetic variation influences the early onset of sporadic colorectal cancer in females. Diet rich in fried red meat intake associated with Arg399Gln-XRCC1, Lys751Gln-XPD and Thr241Met- XRCC3 genetic variations significantly influences the early onset of sporadic colorectal cancer in females.

Interaction between lifestyle factors and the XRCC1, XPD, and XRCC3 genetic variations modulates the risk for sporadic colorectal cancer

Department of Medical Biochemistry, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

3rd Surgical Clinic, Cluj-Napoca, Romania

Revista Romana de Medicina de Laborator

Background: Genetic variations, such as those affecting DNA repair genes, could represent susceptibility factors for sporadic colorectal cancer (CRC) as a...

Interaction between lifestyle factors and the XRCC1, XPD, and XRCC3 genetic variations modulates the risk for sporadic colorectal cancer

Publié en ligne: 25 mars 2014

Pages: 129 - 141

DOI: https://doi.org/10.2478/rrlm-2014-0002

Mots clés
Arg399Gln-XRCC1, Lys751Gln-XPD, Thr241Met-XRCC3, PCR-RFLP, environmental exposure, Romania

This content is open access.

Interaction between lifestyle factors and the XRCC1, XPD, and XRCC3 genetic variations modulates the risk for sporadic colorectal cancer

Publié en ligne: 25 mars 2014

Pages: 129 - 141

DOI: https://doi.org/10.2478/rrlm-2014-0002

Mots clésArg399Gln-XRCC1, Lys751Gln-XPD, Thr241Met-XRCC3, PCR-RFLP, environmental exposure, Romania

This content is open access.

Mots clés
Arg399Gln-XRCC1, Lys751Gln-XPD, Thr241Met-XRCC3, PCR-RFLP, environmental exposure, Romania