<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Studies on caprine leptospirosis using isolation, histochemistry and immunohistochemistry are rare. The role of small ruminants in the epidemiology of leptospirosis is scarce. This study investigated the prevalence of <italic>Leptospira</italic> spp. serovars, and the renal pathology of caprine leptospirosis in slaughterhouses from two states in southwest Nigeria using isolation (IS), Warthin Starry silver (WSs) impregnation and immunohistochemistry (IH). One hundred and sixty-nine kidney samples were randomly obtained from goats between September 2015 and June 2017. Chi-square test was used with a confidence level set at 0.05 to ascertain associations between the positive cases, sex and animal species. Eighty-seven (51.5%) samples were positive on IS, out of which 26/40 and 25/30 were positive on WSs and IH, respectively. Ten (5.9%) kidneys showed macroscopic lesions while interstitial nephritis (48.6%) and tubular nephrosis (64.2%) were the most prominent histopathological changes. The most frequently observed positive reactions were against serovars Hardjo type Prajitno (12/25, 48%), and Gripptotyphosa (5/25, 20%). Other serovars such as Bratislava (2/25, 8%), Canicola (3/25, 12%), Icterohaemorrhagiae (2/25, 8%), and Pomona (1/25, 4.0%) were also detected using IH. The result showed high prevalence of <italic>Leptospira</italic> infection in goats and the possibility of humans contracting the disease. To date, the detection of leptospirosis from kidneys of goats using IS, WSs and IH has not been reported. This study is the first documentation of evidence of pathogenic <italic>Leptospira</italic> species in renal tissues of goats.</p></abstract>

Studies on caprine leptospirosis using isolation, histochemistry and immunohistochemistry are rare. The role of small ruminants in the epidemiology of leptospirosis is scarce. This study investigated the prevalence of Leptospira spp. serovars, and the renal pathology of caprine leptospirosis in slaughterhouses from two states in southwest Nigeria using isolation (IS), Warthin Starry silver (WSs) impregnation and immunohistochemistry (IH). One hundred and sixty-nine kidney samples were randomly obtained from goats between September 2015 and June 2017. Chi-square test was used with a confidence level set at 0.05 to ascertain associations between the positive cases, sex and animal species. Eighty-seven (51.5%) samples were positive on IS, out of which 26/40 and 25/30 were positive on WSs and IH, respectively. Ten (5.9%) kidneys showed macroscopic lesions while interstitial nephritis (48.6%) and tubular nephrosis (64.2%) were the most prominent histopathological changes. The most frequently observed positive reactions were against serovars Hardjo type Prajitno (12/25, 48%), and Gripptotyphosa (5/25, 20%). Other serovars such as Bratislava (2/25, 8%), Canicola (3/25, 12%), Icterohaemorrhagiae (2/25, 8%), and Pomona (1/25, 4.0%) were also detected using IH. The result showed high prevalence of Leptospira infection in goats and the possibility of humans contracting the disease. To date, the detection of leptospirosis from kidneys of goats using IS, WSs and IH has not been reported. This study is the first documentation of evidence of pathogenic Leptospira species in renal tissues of goats.

Detection of Pathogenic <italic>Leptospira</italic> Spp. in Goats from Slaughterhouses in Southwestern Nigeria Using Isolation, Histochemistry and Immunohistochemistry

Department of Public Health and Preventive Medicine

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Detection of Pathogenic  Spp. in Goats from Slaughterhouses in Southwestern Nigeria Using Isolation, Histochemistry and Immunohistochemistry

Studies on caprine leptospirosis using isolation, histochemistry and immunohistochemistry are rare. The role of small ruminants in the epidemiology of...

Detection of Pathogenic Leptospira Spp. in Goats from Slaughterhouses in Southwestern Nigeria Using Isolation, Histochemistry and Immunohistochemistry

Publié en ligne: 15 mars 2021

Pages: 5 - 16

Reçu: 21 févr. 2020

Accepté: 15 sept. 2020

DOI: https://doi.org/10.2478/macvetrev-2020-0031

Mots clés
goats, kidney, leptospirosis, culture isolation, histopathology, immunohistochemistry

© 2021 Olusola Lawrence Ajayi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Detection of Pathogenic Leptospira Spp. in Goats from Slaughterhouses in Southwestern Nigeria Using Isolation, Histochemistry and Immunohistochemistry

Publié en ligne: 15 mars 2021

Pages: 5 - 16

Reçu: 21 févr. 2020

Accepté: 15 sept. 2020

DOI: https://doi.org/10.2478/macvetrev-2020-0031

Mots clésgoats, kidney, leptospirosis, culture isolation, histopathology, immunohistochemistry

© 2021 Olusola Lawrence Ajayi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
goats, kidney, leptospirosis, culture isolation, histopathology, immunohistochemistry