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First isolation of Aerococcus viridans from clinical specimens collected on a pig farm in Poland

Piotr Cybulski

Piotr Cybulski

Goodvalley Agro S.A.Przechlewo, Poland
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Cybulski, Piotr
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Radosław Kondratiuk

Radosław Kondratiuk

Goodvalley Agro S.A.Przechlewo, Poland
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Kondratiuk, Radosław
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Ines Spiekermeier

Ines Spiekermeier

SAN Group Biotech Germany GmbHHöltinghausen, Germany
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Spiekermeier, Ines
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Grzegorz Woźniakowski

Grzegorz Woźniakowski

  • Department of Infectious, Invasive Diseases and Veterinary Administration, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in ToruńToruń, Poland
  • Department of Biochemistry, National Veterinary Research InstitutePuławy, Poland
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Woźniakowski, Grzegorz
  
11 déc. 2024
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Publié en ligne: 11 déc. 2024
Pages: 509 - 514
Reçu: 05 avr. 2024
Accepté: 30 sept. 2024
DOI: https://doi.org/10.2478/jvetres-2024-0058
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© 2024 Piotr Cybulski et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Successful retrieval of Aerococcus viridans from porcine clinical specimens has been rarely described, and data has only been obtained from a few swine-producing countries. Therefore, the aim of this study was the isolation of A. viridans recovered from a specimen originating from a commercial pig farm located in Poland.

Material and Methods

Seven dead 12-week-old pigs weighing 24–26 kg with joint swelling of the hind legs were selected on a modern farrow-to-nursery farm in Poland in October 2023. The research material was joint swabs from one affected limb amputated through the proximal part of the femur. Bacteria were isolated using matrix-assisted laser desorption/ionisation–time-of-flight mass spectrometry. Antimicrobial susceptibility testing of isolates of Staphylococcus aureus, A. viridans and Trueperella pyogenes was performed by disc diffusion and also by minimal inhibitory concentration evaluation in the case of A. viridans. Two pooled samples were screened by PCR for Streptococcus suis, Actinobacillus pleuropneumoniae, Actinobacillus suis, Mycoplasma hyorhinis, M. hyosynoviae and Glaesserella parasuis.

Results

Five samples were positive for bacteria by culture and five isolates were recovered. Staphylococcus aureus was identified in three samples and T. pyogenes and A. viridans in one each. Pooled samples were G. parasuis-, M. hyorhinis-, M. hyosynoviae-, Actinobacillus pleuropneumoniae-, Actinobacillus suis- and S. suis-negative in the PCR. The A. viridans isolated was susceptible to beta-lactams and gentamicin. Ten representative nucleotide contigs from the 500 obtained showed similarity as high as 97.5% to GenBank reference strains.

Conclusion

To the best of our knowledge, this is the first identification of A. viridans in clinically affected Polish pigs. It elevates the importance of uncommon pathogens, including A. viridans, in the development of lameness in pigs. This research emphasises the role of modern diagnostic tools for accurate identification of swine pathogens. Further research would prove beneficial to elucidate the mechanisms of A. viridans infection, its prevalence, pathogenicity and virulence factors.
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire
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