Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland

Lotmaria passim (L. passim) is a single-celled flagellate which colonises the bee gastrointestinal tract and is highly prevalent in honey bees. This parasite is associated with colony losses. Honey bee (Apis mellifera) colonies were sampled from five apiaries in the north-eastern part of Poland for the phylogenetic analysis of L. passim.

Material and Methods

Each apiary consisted of approximately 60 bee colonies, of which 20 were randomly selected. Samples of 60 differently aged worker bees were collected from each colony and pooled. A total of 100 bee colonies from five apiaries were examined. Protozoa of the Trypanosomatidae family were identified by PCR. L. passim was detected in 47 (47%) of the samples. The 18S ribosomal (r) RNA amplicons of L. passim were sequenced by a commercial service. Their sequences were analysed with BLASTN and noted to be compatible with the GenBank sequences of this region of the organism’s genome. A sequence analysis was performed using the BioEdit Sequence Alignment Editor and Clustal W software.

Results

The amplicon sequences of L. passim were 100% homologous with the sequences deposited in GenBank under accession numbers KM066243.1., KJ684964.1 and KM980181.1.

Conclusion

This is the first study to perform a phylogenetic analysis of L. passim in Polish honey bees. The analysis demonstrated high levels of genetic similarity between isolates of L. passim colonising apiaries in the north-eastern region of Poland.

Langue:: Anglais

Périodicité:: 4 fois par an
Sujets de la revue:: Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire

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Phylogenetic analysis of the trypanosomatid parasite Lotmaria passim in honey bees (Apis mellifera) in Poland

Maria Iller

Karolina Lipczyńska-Ilczuk

Rajmund Sokół

Grzegorz Borsuk

Agata Bancerz-Kisiel

Publié en ligne: 23 mars 2024

Pages: 123 - 127

Reçu: 04 sept. 2023

Accepté: 13 mars 2024

DOI: https://doi.org/10.2478/jvetres-2024-0018

Mots clésbee parasite, phylogenetic analysis

© 2024 Maria Iller et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Material and Methods

Results

Conclusion

Mots clés
bee parasite, phylogenetic analysis