<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>This study was an essential part of the method’s transfer from the National Reference Laboratory to six regional laboratories in Poland working in the official survey of mycotoxins in feed. The laboratories received a batch of standard solutions, blank samples and quality control materials on which to perform analysis with one procedure and different liquid chromatography–tandem mass spectrometry conditions.</p>
</sec>
<sec>
<h3>Results</h3>
<p>The validation results show good precision (reproducibility coefficient of variation 3.7–20.5%) and accuracy of the method (recovery 89–120% and trueness 94–103%) and sufficient skills of the laboratory personnel.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>The study is an example of the successful transfer of the method among laboratories.</p>
</sec>
</abstract>

Introduction
The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds.


Material and Methods
This study was an essential part of the method’s transfer from the National Reference Laboratory to six regional laboratories in Poland working in the official survey of mycotoxins in feed. The laboratories received a batch of standard solutions, blank samples and quality control materials on which to perform analysis with one procedure and different liquid chromatography–tandem mass spectrometry conditions.


Results
The validation results show good precision (reproducibility coefficient of variation 3.7–20.5%) and accuracy of the method (recovery 89–120% and trueness 94–103%) and sufficient skills of the laboratory personnel.


Conclusion
The study is an example of the successful transfer of the method among laboratories.

Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of...

Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories

Publié en ligne: 20 sept. 2023

Pages: 397 - 406

Reçu: 23 févr. 2023

Accepté: 18 juil. 2023

DOI: https://doi.org/10.2478/jvetres-2023-0045

Mots clés
mycotoxins, mass spectrometry, feed, validation, inter-laboratory comparison

© 2023 Piotr Jedziniak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories

Publié en ligne: 20 sept. 2023

Pages: 397 - 406

Reçu: 23 févr. 2023

Accepté: 18 juil. 2023

DOI: https://doi.org/10.2478/jvetres-2023-0045

Mots clésmycotoxins, mass spectrometry, feed, validation, inter-laboratory comparison

© 2023 Piotr Jedziniak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
mycotoxins, mass spectrometry, feed, validation, inter-laboratory comparison