Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds
et | 20 sept. 2023
À propos de cet article
Publié en ligne: 20 sept. 2023
Pages: 347 - 352
Reçu: 20 févr. 2023
Accepté: 04 juil. 2023
DOI: https://doi.org/10.2478/jvetres-2023-0041
Mots clés
© 2023 Monika Beinhauerova et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Examination of fresh environmental samples for the presence of Mycobacterium avium subsp. paratuberculosis (MAP) using an Actiphage–IS900 qPCR assay, direct IS900 qPCR, and a conventional phage amplification assay (PA)
| Sample no. | Sample characteristics | Direct IS |
Conventional PA (PFU/g) |
Actiphage–IS |
|---|---|---|---|---|
| 1 | Soil | 5.42 × 102 | 4.80 × 102 | – |
| 2 | Soil | 1.09 × 102 | 2.18 × 102 | – |
| 3 | Soil | 1.02 × 102 | 2.88 × 102 | – |
| 4 | Soil | 4.20 × 102 | – | – |
| 5 | Cobweb | 1.20 × 102 | – | – |
| 6 | Soil/plants | 1.64 × 100 | 7.54 × 103 | 1.84 × 100 |
| 7 | Soil | 5.88 × 100 | 3.06 × 102 | – |
| 8 | Soil | 6.56 × 100 | 1.09 × 102 | – |
| 9 | Soil | 2.40 × 100 | 4.36 × 101 | – |
Examination of frozen environmental samples for the presence of Mycobacterium avium subsp. paratuberculosis (MAP) using an Actiphage–IS900 qPCR assay, direct IS900 qPCR, a conventional phage amplification assay (PA) and culture
| Sample no. | Sample characteristics | Time frozen | Direct |
Conventional PA (PFU/g) |
Culture |
Actiphage–IS |
|---|---|---|---|---|---|---|
| 1 | Soil | 11 years | 6.43 × 103 | 6.98 × 102 | – | – |
| 2 | Soil | 11 years | 1.18 × 103 | 8.72 × 102 | – | – |
| 3 | Soil | 11 years | 1.16 × 102 | 2.88 × 103 | – | – |
| 4 | Soil | 11 years | 2.11 × 102 | 1.92 × 103 | – | – |
| 5 | Soil | 11 years | 1.30 × 102 | 2.01 × 102 | – | – |
| 6 | Soil | 11 years | 7.94 × 102 | 2.62 × 102 | – | – |
| 7 | Soil | 11 years | 3.48 × 102 | 7.85 × 102 | – | – |
| 8 | Roots | 11 years | 1.07 × 102 | 3.49 × 102 | – | – |
| 9 | Soil | 11 years | 7.39 × 102 | 2.62 × 102 | – | – |
| 10 | Roots | 11 years | 1.68 × 102 | 3.49 × 102 | – | – |
| 11 | Soil | 11 years | 1.61 × 102 | 2.62 × 102 | – | – |
| 12 | Roots | 11 years | 4.36 × 102 | 6.98 × 102 | – | – |
| 13 | Roots | 11 years | 6.41 × 102 | 2.27 × 102 | – | – |
| 14 | Soil | 11 years | 9.81 × 102 | 6.10 × 102 | – | – |
| 15 | Soil | 11 years | 4.09 × 102 | 1.74 × 102 | – | – |
| 16 | Roots | 11 years | 1.57 × 102 | 5.58 × 102 | – | – |
| 17 | Soil | 8 years | 4.40 × 102 | 4.36 × 102 | – | – |
| 18 | Detritus | 8 years | 1.25 × 102 | 4.36 × 101 | – | – |
| 19 | Feed residues | 8 years | 1.94 × 102 | – | – | – |
| 20 | Soil | 11 years | 2.67 × 101 | 8.72 × 101 | – | – |
| 21 | Soil | 11 years | 7.26 × 101 | 1.13 × 103 | – | – |
| 22 | Soil | 11 years | 8.93 × 101 | 5.67 × 102 | – | – |
| 23 | Soil | 11 years | 6.11 × 101 | 3.49 × 103 | – | – |
| 24 | Soil | 11 years | 7.95 × 101 | 1.74 × 102 | – | – |
| 25 | Roots | 11 years | 6.44 × 101 | 5.23 × 101 | – | – |
| 26 | Soil | 11 years | 7.90 × 101 | 8.72 × 100 | – | – |
| 27 | Soil | 11 years | 2.70 × 101 | 1.40 × 102 | – | – |
| 28 | Roots | 11 years | 9.91 × 101 | 1.74 × 102 | – | – |