<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec>
<h3>Introduction</h3>
<p>Bioactive proteins and peptides generated from fruit, vegetables, meat or fish have great potential as functional food or substitutes for antibiotics. In recent years it has also been demonstrated that the fungus kingdom could be a source of these compounds. The study investigated the bioactivity of an extract of the lignicolous fungus <italic>Trametes versicolor</italic> and its hydrolysate.</p>
</sec>
<sec>
<h3>Material and Methods</h3>
<p>The fungus was collected in a mixed forest in October, extracted and hydrolysed. To inspect the protein and peptide profiles before and after hydrolysis, matrix-assisted laser desorption/ionisation–time-of-flight mass spectrometric analysis was performed. To evaluate the antioxidant properties of the preparations, 2,2-diphenyl-1-picrylhydrazyl (DPPH<sup>•</sup>) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS<sup>•+</sup>) radical scavenging assays were used. The activity of the fungus extract and hydrolysate against <italic>Aeromonas veronii</italic>, <italic>Bacillus cereus</italic>, <italic>Enterococcus faecalis</italic>, <italic>Enterococcus faecium</italic>, <italic>Escherichia coli</italic>, <italic>Klebsiella pneumoniae</italic>, <italic>Pseudomonas aeruginosa</italic>, <italic>Salmonella</italic> Typhimurium, <italic>Staphylococcus aureus</italic>, <italic>Staphylococcus epidermidis</italic>, <italic>Streptococcus agalactiae</italic>, <italic>Streptococcus dysgalactiae</italic>, and <italic>Streptococcus uberis</italic> was determined by the minimum inhibitory concentration and minimum bactericidal concentration values.</p>
</sec>
<sec>
<h3>Results</h3>
<p>The extract and its hydrolysate showed almost 100% ABTS<sup>•+</sup> and DPPH<sup>•</sup> radical scavenging with a low half maximal inhibitory concentration. The water extract and hydrolysate of <italic>T. versicolor</italic> exhibited antimicrobial activity against two <italic>S. aureus</italic> strains, <italic>E. coli</italic>, <italic>P. aeruginosa</italic> and <italic>Salmonella</italic> Typhimurium.</p>
</sec>
<sec>
<h3>Conclusion</h3>
<p>These results provide compelling evidence that the analysed fungus extract and its hydrolysate hold promise with their antibacterial and antioxidant properties.</p>
</sec>
</abstract>

Introduction
Bioactive proteins and peptides generated from fruit, vegetables, meat or fish have great potential as functional food or substitutes for antibiotics. In recent years it has also been demonstrated that the fungus kingdom could be a source of these compounds. The study investigated the bioactivity of an extract of the lignicolous fungus Trametes versicolor and its hydrolysate.


Material and Methods
The fungus was collected in a mixed forest in October, extracted and hydrolysed. To inspect the protein and peptide profiles before and after hydrolysis, matrix-assisted laser desorption/ionisation–time-of-flight mass spectrometric analysis was performed. To evaluate the antioxidant properties of the preparations, 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radical scavenging assays were used. The activity of the fungus extract and hydrolysate against Aeromonas veronii, Bacillus cereus, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella Typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Streptococcus uberis was determined by the minimum inhibitory concentration and minimum bactericidal concentration values.


Results
The extract and its hydrolysate showed almost 100% ABTS•+ and DPPH• radical scavenging with a low half maximal inhibitory concentration. The water extract and hydrolysate of T. versicolor exhibited antimicrobial activity against two S. aureus strains, E. coli, P. aeruginosa and Salmonella Typhimurium.


Conclusion
These results provide compelling evidence that the analysed fungus extract and its hydrolysate hold promise with their antibacterial and antioxidant properties.

Antioxidant and antimicrobial properties of an extract rich in proteins obtained from <italic>Trametes versicolor</italic>

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antioxidant and antimicrobial properties of an extract rich in proteins obtained from 

Introduction
Bioactive proteins and peptides generated from fruit, vegetables, meat or fish have great potential as functional food or substitutes for...

Antioxidant and antimicrobial properties of an extract rich in proteins obtained from Trametes versicolor

Publié en ligne: 16 juin 2023

Pages: 209 - 218

Reçu: 25 avr. 2023

Accepté: 12 juin 2023

DOI: https://doi.org/10.2478/jvetres-2023-0036

Mots clés
bioactive proteins, bioactive peptides, antioxidant properties, antibacterial properties

© 2023 K. Michalak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antioxidant and antimicrobial properties of an extract rich in proteins obtained from Trametes versicolor

Publié en ligne: 16 juin 2023

Pages: 209 - 218

Reçu: 25 avr. 2023

Accepté: 12 juin 2023

DOI: https://doi.org/10.2478/jvetres-2023-0036

Mots clésbioactive proteins, bioactive peptides, antioxidant properties, antibacterial properties

© 2023 K. Michalak et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
bioactive proteins, bioactive peptides, antioxidant properties, antibacterial properties