Accès libre

Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams

Jarosław Kaba

Jarosław Kaba

Division of Veterinary Epidemiology and EconomicsWarsaw, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Kaba, Jarosław
, 
Michał Czopowicz

Michał Czopowicz

Division of Veterinary Epidemiology and EconomicsWarsaw, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Czopowicz, Michał
, 
Lucjan Witkowski

Lucjan Witkowski

Division of Veterinary Epidemiology and EconomicsWarsaw, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Witkowski, Lucjan
, 
Olga Szaluś-Jordanow

Olga Szaluś-Jordanow

Department of Small Animal Diseases with Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences–SGGWWarsaw, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Szaluś-Jordanow, Olga
, 
Marcin Mickiewicz

Marcin Mickiewicz

Division of Veterinary Epidemiology and EconomicsWarsaw, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Mickiewicz, Marcin
, 
Iwona Markowska-Daniel

Iwona Markowska-Daniel

Division of Veterinary Epidemiology and EconomicsWarsaw, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Markowska-Daniel, Iwona
, 
Ryszard Puchała

Ryszard Puchała

Applied Physiology Unit, Military Institute of Hygiene and EpidemiologyWarsaw, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Puchała, Ryszard
 et 
Emilia Bagnicka

Emilia Bagnicka

Institute of Genetics and Animal Biotechnology, Polish Academy of SciencesMagdalenka, Poland
Recherchez cet auteur sur
Sciendo|Google Scholar
Bagnicka, Emilia
  
26 déc. 2022
Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams's Cover Image
À propos de cet article
Article précédent
Article suivant
Citez
Télécharger la couverture
Publié en ligne: 26 déc. 2022
Pages: 511 - 521
Reçu: 13 sept. 2022
Accepté: 07 déc. 2022
DOI: https://doi.org/10.2478/jvetres-2022-0071
Mots clés
© 2022 J. Kaba et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Timeline of the serological status of 13 goats that seroconverted during the study. The orange band is the duration of maternal antibodies, the blue band is the time of seronegativity in both ELISAs, and the red band is the time of seropositivity in at least one ELISA. The direct transition from the period of maternal antibodies to the period of active seroreactivity by goat no. 28 results from the overlapping seropositivities of this goat in the two ELISAs used – see Fig. 3 for details
Timeline of the serological status of 13 goats that seroconverted during the study. The orange band is the duration of maternal antibodies, the blue band is the time of seronegativity in both ELISAs, and the red band is the time of seropositivity in at least one ELISA. The direct transition from the period of maternal antibodies to the period of active seroreactivity by goat no. 28 results from the overlapping seropositivities of this goat in the two ELISAs used – see Fig. 3 for details

Fig. 2

Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in intermittent (goat no. 9 and goat no. 18) and late seroreactors (goat no. 13 and goat no. 20). Dashed lines indicate the manufacturer’s cut-off value used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of the sp-iELISA (red) and the SU-cELISA (green) or the times at which an intermittent seroreactor was seropositive
Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in intermittent (goat no. 9 and goat no. 18) and late seroreactors (goat no. 13 and goat no. 20). Dashed lines indicate the manufacturer’s cut-off value used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of the sp-iELISA (red) and the SU-cELISA (green) or the times at which an intermittent seroreactor was seropositive

Fig. 3

Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in early and stable seroreactors. Dashed lines indicate the manufacturer’s cut-off values used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of sp-iELISA (red) and SU-cELISA (green)
Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in early and stable seroreactors. Dashed lines indicate the manufacturer’s cut-off values used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of sp-iELISA (red) and SU-cELISA (green)

Fig. 4

Quantitative results of an indirect ELISA coated with the mixture of synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) of 18 seronegative goats in the first 24 months of their lives. The red dashed line indicates the manufacturer’s cut-off value of S/P% = 50%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months
Quantitative results of an indirect ELISA coated with the mixture of synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) of 18 seronegative goats in the first 24 months of their lives. The red dashed line indicates the manufacturer’s cut-off value of S/P% = 50%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months

Fig. 5

Quantitative results of a competitive ELISA coated with viral surface glycoprotein (SU-cELISA; percent inhibition, PI) of 18 seronegative goats in the first 24 months of their lives. The green dashed line indicates the manufacturer’s cut-off value of PI = 35%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months
Quantitative results of a competitive ELISA coated with viral surface glycoprotein (SU-cELISA; percent inhibition, PI) of 18 seronegative goats in the first 24 months of their lives. The green dashed line indicates the manufacturer’s cut-off value of PI = 35%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months

Dam Number of kids born in a particular year with number of seroreactors in parentheses Year the dam was born Year the dam seroconverted or first tested seropositive
2014 2015
1 1 (0) - 2005 2007
2 1 (0) - 2009 2011
3 1 (0) 1 (0) 2009 2012
4 - 2 (2a) 2009 2014
5 2 (0) - 2010 2011
6 1 (1a) - 2010 2013
7 2 (1c) - 2006 2013
8 1 (0) - 2011 2013
9 1 (1c) - 2011 2013
10 1 (0) - 2011 2013
11 - 2 (1a) 2012 2014
12 1 (1b) 2 (2a) 2008 2013
13 2 - 2007 2010
14 1 (0) - 2008 2010
15 1 (0) - 2008 2013
16 2 (1b) 2 (1a) 2008 2013
17 2 (1a) 2 (1a) 2009 2013
Total 20 (2a, 2b, 2c) 11 (7 a)
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire
RSS Feed de la revue