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High prevalence of Plasmodium infection in fighting cocks in Thailand determined with a molecular method

Kotchaphon Vaisusuk

Kotchaphon Vaisusuk

Department of Veterinary Technology and Veterinary Nursing, Faculty of Agricultural Technology, Rajabhat Maha Sarakham UniversityMaha Sarakham, Thailand
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Vaisusuk, Kotchaphon
, 
Wasupon Chatan

Wasupon Chatan

Faculty of Veterinary SciencesMaha Sarakham, Thailand
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Chatan, Wasupon
, 
Tossapol Seerintra

Tossapol Seerintra

Faculty of Veterinary SciencesMaha Sarakham, Thailand
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Seerintra, Tossapol
 et 
Supawadee Piratae

Supawadee Piratae

One Health Research Unit, Faculty of Veterinary Sciences, Mahasarakham UniversityMaha Sarakham, Thailand
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Piratae, Supawadee
  
15 sept. 2022
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Publié en ligne: 15 sept. 2022
Pages: 373 - 379
Reçu: 22 avr. 2022
Accepté: 02 sept. 2022
DOI: https://doi.org/10.2478/jvetres-2022-0049
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© 2022 K. Vaisusuk et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Avian malaria caused by Plasmodium and the malaria-like parasites of the genus Haemoproteus has been regularly described in multiple regions worldwide. These parasites significantly affect many avian taxa, including domestic chickens and fighting cocks. There are limited epidemiological studies of these blood parasites in vertebrate hosts, especially in Thailand.

Material and Methods

This study used microscopic examination of blood samples and PCR amplification exploiting primers for nucleotide sequences of Plasmodium or Haemoproteus species based on the cytochrome b gene to determine the occurrence of Plasmodium spp. in fighting cocks.

Results

Examination of 249 blood samples of fighting cocks revealed that 41.37% (103/249) were positive for malaria by microscopic examination and 88.76% (221/249) were positive by DNA amplification. Sequencing and DNA analysis of 61 PCR products revealed that infection by Plasmodium juxtanucleare was the most common avian malaria in fighting cocks in Thailand followed by infections by Plasmodium gallinaceum; however, Haemoproteus infection was not discovered.

Conclusion

This study indicated that plasmodiasis is widespread in fighting cocks in Thailand although the prevalence was not clearly determined; therefore, prevention and control strategies for these protozoa should be improved, especially those for avoiding vector exposure and eliminating mosquito breeding sites.
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire
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