<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2022-0038_s_005"><h3>Introduction</h3>
<p>Bovine tuberculosis is one of the most dangerous zoonotic diseases. Despite the near-complete elimination of the disease from cattle breeding in Poland achieved in 2009, its re-emergence is now observed. Globally, the number of human cases is underestimated and the importance of free-living animals as reservoirs of tuberculosis is growing. As a species highly susceptible to <italic>Mycobacterium tuberculosis</italic> complex infection, the European bison (Bison bonasus) has a role in the transmission of the disease in Poland. The purpose of the investigation was to assess the epidemiological situation of tuberculosis in Polish European bison serologically.</p></sec>
<sec id="j_jvetres-2022-0038_s_006"><h3>Material and Methods</h3>
<p>A total of 460 serum samples were collected from 436 European bison from 15 out of 26 national populations between 2013 and 2020. An <italic>M. bovis</italic> ELISA was used, and its sensitivity and specificity were assessed with an eyelid tuberculin skin test (TST) and interferon gamma release assay (IGRA).</p></sec>
<sec id="j_jvetres-2022-0038_s_007"><h3>Results</h3>
<p><italic>Mycobacterium bovis</italic> antibodies were detected in nine serum samples. The presence of antibodies was found in two animals from the Białowieża Forest (1.2% of the population), and one each from the Borecka Forest (2.4%) and the Warsaw Zoo (14.3%). One European bison among the 14 sampled (7.1%) from Smardzewice was positive on five occasions. Other samples from Smardzewice and the Bieszczady Mountains, where tuberculosis had previously been reported, were negative.</p></sec>
<sec id="j_jvetres-2022-0038_s_008"><h3>Conclusion</h3>
<p>ELISA testing is an effective, easy and cost-efficient tool for monitoring of tuberculosis-naïve populations. Serological testing in tuberculosis control programmes can significantly improve the detection of infected herds. Antibody ELISAs may supplement TST and IGRA, but cannot replace them.</p>
</sec>
</abstract>

Introduction
Bovine tuberculosis is one of the most dangerous zoonotic diseases. Despite the near-complete elimination of the disease from cattle breeding in Poland achieved in 2009, its re-emergence is now observed. Globally, the number of human cases is underestimated and the importance of free-living animals as reservoirs of tuberculosis is growing. As a species highly susceptible to Mycobacterium tuberculosis complex infection, the European bison (Bison bonasus) has a role in the transmission of the disease in Poland. The purpose of the investigation was to assess the epidemiological situation of tuberculosis in Polish European bison serologically.
Material and Methods
A total of 460 serum samples were collected from 436 European bison from 15 out of 26 national populations between 2013 and 2020. An M. bovis ELISA was used, and its sensitivity and specificity were assessed with an eyelid tuberculin skin test (TST) and interferon gamma release assay (IGRA).
Results
Mycobacterium bovis antibodies were detected in nine serum samples. The presence of antibodies was found in two animals from the Białowieża Forest (1.2% of the population), and one each from the Borecka Forest (2.4%) and the Warsaw Zoo (14.3%). One European bison among the 14 sampled (7.1%) from Smardzewice was positive on five occasions. Other samples from Smardzewice and the Bieszczady Mountains, where tuberculosis had previously been reported, were negative.
Conclusion
ELISA testing is an effective, easy and cost-efficient tool for monitoring of tuberculosis-naïve populations. Serological testing in tuberculosis control programmes can significantly improve the detection of infected herds. Antibody ELISAs may supplement TST and IGRA, but cannot replace them.

Is serological monitoring a fit-for-purpose tool to assess the epidemiological situation of tuberculosis in the sylvatic species of European bison (<italic>Bison bonasus</italic>) in Poland?

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Is serological monitoring a fit-for-purpose tool to assess the epidemiological situation of tuberculosis in the sylvatic species of European bison () in Poland?

Introduction
Bovine tuberculosis is one of the most dangerous zoonotic diseases. Despite the near-complete elimination of the disease from cattle breeding in...

Is serological monitoring a fit-for-purpose tool to assess the epidemiological situation of tuberculosis in the sylvatic species of European bison (Bison bonasus) in Poland?

Publié en ligne: 30 juil. 2022

Pages: 333 - 344

Reçu: 14 mars 2022

Accepté: 12 juil. 2022

DOI: https://doi.org/10.2478/jvetres-2022-0038

Mots clés
bovine tuberculosis, European bison, serology, ELISA, tuberculin skin test, interferon gamma release assay

© 2022 Michał K. Krzysiak et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Is serological monitoring a fit-for-purpose tool to assess the epidemiological situation of tuberculosis in the sylvatic species of European bison (Bison bonasus) in Poland?

Publié en ligne: 30 juil. 2022

Pages: 333 - 344

Reçu: 14 mars 2022

Accepté: 12 juil. 2022

DOI: https://doi.org/10.2478/jvetres-2022-0038

Mots clésbovine tuberculosis, European bison, serology, ELISA, tuberculin skin test, interferon gamma release assay

© 2022 Michał K. Krzysiak et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
bovine tuberculosis, European bison, serology, ELISA, tuberculin skin test, interferon gamma release assay