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Effects of bisphenol A and bisphenol F on porcine uterus contractility

Aleksandra Zygmuntowicz
Aleksandra Zygmuntowicz
, 
Włodzimierz Markiewicz
Włodzimierz Markiewicz
, 
Tomasz Grabowski
Tomasz Grabowski
 et 
Jerzy Jaroszewski
Jerzy Jaroszewski
   | 05 juil. 2022
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Publié en ligne: 05 juil. 2022
Pages: 257 - 265
Reçu: 10 févr. 2022
Accepté: 01 juin 2022
DOI: https://doi.org/10.2478/jvetres-2022-0031
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© 2022 A. Zygmuntowicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Scheme presenting the sequence of treatment of the uterine strips. ACh – acetylocholine; BPA – bisphenol A; BPF – bisphenol F. Concentrations of the applied substances are given in moles
Scheme presenting the sequence of treatment of the uterine strips. ACh – acetylocholine; BPA – bisphenol A; BPF – bisphenol F. Concentrations of the applied substances are given in moles

Fig. 2

Representative diagrams showing contractile activity of the myometrial strips collected from immature pigs (A and D), cyclic pigs on days 12–14 of the oestrous cycle (B and E) and early pregnant pigs on days 12–16 of pregnancy (C and F) and treated with bisphenol A (BPA) and bisphenol F (BPF) at concentrations of 10−13 –10−1 mol/L
Representative diagrams showing contractile activity of the myometrial strips collected from immature pigs (A and D), cyclic pigs on days 12–14 of the oestrous cycle (B and E) and early pregnant pigs on days 12–16 of pregnancy (C and F) and treated with bisphenol A (BPA) and bisphenol F (BPF) at concentrations of 10−13 –10−1 mol/L

Fig. 3

Influence of bisphenol A on the tension (A), amplitude (B) and frequency (C) of contractions of the porcine uterine myometrial strips collected from immature pigs, cyclic pigs on days 12–14 of the oestrous cycle and early pregnant pigs on days 12–16 of pregnancy (n = 12 in each group). * – P < 0.05; ** – P < 0.01; *** – P < 0.001 compared to the contractile activity before the treatment
Influence of bisphenol A on the tension (A), amplitude (B) and frequency (C) of contractions of the porcine uterine myometrial strips collected from immature pigs, cyclic pigs on days 12–14 of the oestrous cycle and early pregnant pigs on days 12–16 of pregnancy (n = 12 in each group). * – P < 0.05; ** – P < 0.01; *** – P < 0.001 compared to the contractile activity before the treatment

Fig. 4

Influence of bisphenol F on the tension (A), amplitude (B) and frequency (C) of contractions of the porcine uterine myometrial strips collected from immature pigs, cyclic pigs on days 12–14 of the estrous cycle and early pregnant pigs on days 12–16 of pregnancy (n = 12 in each group). *P < 0.05; **P < 0.01; ***P < 0.001 compared to the contractile activity before the treatment
Influence of bisphenol F on the tension (A), amplitude (B) and frequency (C) of contractions of the porcine uterine myometrial strips collected from immature pigs, cyclic pigs on days 12–14 of the estrous cycle and early pregnant pigs on days 12–16 of pregnancy (n = 12 in each group). *P < 0.05; **P < 0.01; ***P < 0.001 compared to the contractile activity before the treatment

Dose-response parameters (arithmetic mean with 90% confidence interval), based on the tension, frequency and amplitude of contractions in immature pigs, cyclic pigs on days 12–14 of the estrous cycle and early pregnant pigs on days 12–16 of pregnancy treated with concentrations of bisphenol A and F increasing from 10−13 to 10−1 M

Parameters Tension Amplitude Frequency
Immature Cyclic Pregnant Immature Cyclic Pregnant Immature Cyclic Pregnant
BPA 8.887 (8.433–9.341) 6.451 (6.201–6.700) 16.73 (15.64–17.81) 24.82 (23.34–26.30) 23.09 (22.21–23.97) 27.20 (25.66–28.73) 12.74 (11.17–14.31) 6.037 (5.713–6.360) 8.235 (6.842–9.629)
Emax 8.340 BPF (7.948–8.731) 10.03 (9.776–10.29) 8.386 (7.657–9.114) 22.00 (21.45–22.56) 28.77 (28.23–29.31) 23.68 (21.24–26.13) 9.702 (8.885–10.52) 5.055 (4.808–5.302) 4.978 (4.687–5.270)
5.966 BPA (5.071–6.861) 4.321 (2.913–5.728) 10.09 (7.973–12.21) 10.98 (8.672–13.28) 12.11 (10.95–13.27) 21.56 (21.01–22.11) 4.188 (3.280–5.096) 2.676 (2.122–3.229) 4.325 (3.958–4.692)
E0 BPF 6.756 (6.561–6.950) 8.619 (8.504–8.735) 2.383 (0.1448–4.622) −0.07270 (−0.4302–0.2848) −1.726 (−2.557−0.8954) 10.30 (8.076–12.53) 0.1706 (−0.2099–0.5510) 0.9818 (0.8362–1.127) −0.7278 (−1.361−0.09438)
Span BPA 2.921 (1.937–3.905) 2.130 (0.7245–3.536) 6.632 (4.296–8.968) 13.84 (11.24–16.45) 10.98 (9.603–12.36) 5.635 (4.026–7.245) 8.554 (6.768–10.34) 3.361 (2.752–3.970) 3.910 (2.478–5.342)
BPF 1.584 (1.153–2.015) 1.415 (1.135–1.695) 6.002 (3.812–8.192) 22.08 (21.44–22.72) 30.50 (29.55–31.44) 13.38 (10.15–16.61) 9.531 (8.639–10.42) 4.073 (3.791–4.355) 5.706 (5.052–6.360)
−3.775 BPA (−4.633−2.916) −1.758 (−2.769−0.7461) −3.807 (−4.703−2.911) −2.192 (−2.608−1.776) −2.477 (−2.793−2.161) −10.17 (−10.87−9.477) −5.665 (−6.325−5.004) −2.061 (−2.441−1.681) −10.11 (−11.01−9.210)
LogIC50 −6.622 BPF (−7.489−5.755) −7.314 (−7.925−6.703) −1.634 (−2.302−0.9650) −4.799 (−5.226−4.372) −2.225 (−2.294−2.155) −5.113 (−5.772−4.453) −7.070 (−7.351−6.789) −7.292 (−7.497−7.087) −1.830 (−2.057−1.604)
BPA 1.554 −11.34 24.19 9.012 −5.332 −11.53 1.141 −30.13 −18.68
AIC BPF −37.77 −50.61 −8.395 −19.02 −17.05 14.35 −19.84 −46.59 −32.40
BPA 0.6702 0.4082 1.601 0.8928 0.5143 0.3188 0.6596 0.1981 0.3078
RMSE BPF 0.1477 0.09013 0.4571 0.2268 0.3277 1.072 0.2943 0.08467 0.1816
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