Accès libre

Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China

Wang Xifeng
Wang Xifeng
, 
Zhang Kai
Zhang Kai
, 
Zhang Guowu
Zhang Guowu
, 
Li Zhiyuan
Li Zhiyuan
, 
Shang Yunxia
Shang Yunxia
, 
Ning Chengcheng
Ning Chengcheng
, 
Ji Chunhui
Ji Chunhui
, 
Qiao Jun
Qiao Jun
, 
Meng Qingling
Meng Qingling
 et 
Cai Xuepeng
Cai Xuepeng
   | 22 avr. 2022
Journal of Veterinary Research's Cover Image
À propos de cet article
Article précédent
Article suivant
Citez
Publié en ligne: 22 avr. 2022
Pages: 199 - 207
Reçu: 14 sept. 2021
Accepté: 18 mars 2022
DOI: https://doi.org/10.2478/jvetres-2022-0018
Mots clés
© 2022 W. Xifeng et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Morphological identification of parasites from sheep. A – parasites found in bile ducts of sheep; B – parasites fixed on slides with 75% alcohol after washing with phosphate buffered saline (0.01M, pH 7.2–7.4)
Morphological identification of parasites from sheep. A – parasites found in bile ducts of sheep; B – parasites fixed on slides with 75% alcohol after washing with phosphate buffered saline (0.01M, pH 7.2–7.4)

Fig. 2

Median-joining network of F. hepatica isolates based on cox1 (A) and nad1 (B) genes’ haplotypes. The size of the circle indicates the frequency of the haplotype. The colour of the circle indicates the origin of the population. The green dot represents the default vertex
Median-joining network of F. hepatica isolates based on cox1 (A) and nad1 (B) genes’ haplotypes. The size of the circle indicates the frequency of the haplotype. The colour of the circle indicates the origin of the population. The green dot represents the default vertex

Fig. 3

Phylogenetic tree of haplotypes of different geographic populations of F. hepatica in Xinjiang. The tree is based on cox1 (A) and nad1 (B) and constructed with the neighbour-joining method
Phylogenetic tree of haplotypes of different geographic populations of F. hepatica in Xinjiang. The tree is based on cox1 (A) and nad1 (B) and constructed with the neighbour-joining method

Fig. 4

Mismatch distribution of haplotypes of the cox1 (A) and nad1 (B) genes. The number of nucleotide differences between pairs of sequences is indicated along the x–axis, and their frequency along the y–axis. Obs – observed value; Exp – expected value
Mismatch distribution of haplotypes of the cox1 (A) and nad1 (B) genes. The number of nucleotide differences between pairs of sequences is indicated along the x–axis, and their frequency along the y–axis. Obs – observed value; Exp – expected value

Molecular variation analysis based on cox1 and nad1 gene sequences

Source of variation Gene Degrees of freedom Sum of squares Variance components Percentage of variation (%)
Within cox1 141 62.933 0.44634 Vb 99.70
populations nad1 141 55.711 0.39511 Vb 96.48
Among cox1 2 0.990 0.00133 Va 0.30
populations nad1 2 1.844 0.01442 Va 3.52
Total cox1 143 63.924 0.44767
nad1 143 57.556 0.40953
F–statistic cox1 0.04451, P > 0.05
nad1 0.03520, P > 0.05

Genetic diversity parameters of the three geographic populations of F. hepatica

Genes Populations N h Hd K π
Urumqi 45 18 0.909 ± 0.00089 3.160 0.00637
cox1 Hami 90 23 0.890 ± 0.00047 3.280 0.00661
Kuqa 9 6 0.833 ± 0.01600 2.722 0.00549
Total 144 34 0.894 ± 0.00034 3.287 0.00663
Urumqi 45 12 0.835 ± 0.00118 2.638 0.00400
nad1 Hami 90 25 0.7590 ± 0.00218 2.778 0.00421
Kuqa 9 6 0.889 ± 0.00828 2.583 0.00391
Total 144 33 0.805 ± 0.00086 2.969 0.00450

Neutrality indices based on cox1 and nad1 genes of F. hepatica in different geographical regions of Xinjiang

Population cox1 nad1
Tajima’s D Fu’s Fs Tajima’s D Fu’s Fs
Urumqi –0.74410 –7.514 –0.72079 –2.547
Hami –0.36276 –8.659 –1.94511* –13.448
Kuqa –0.34120 –1.395 –0.55536 –1.522
Total –1.01276 –19.204 –1.92702* –19.881
eISSN:
2450-8608
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
RSS Feed de la revue