Analyte | Retention time (min) | Precursor ion (m/z) | Ion transition | Declustering potential | Collision energy (eV) |
---|---|---|---|---|---|
Brombuterol | 4.2 | 366.8 | 213.9 | 56 | 43 |
292.8 | 47 | ||||
Clenbuterol | 4.1 | 276.8 | 203.0 | 76 | 23 |
132.1 | 47 | ||||
Isoxsuprine | 4.3 | 302.2 | 150.0 | 61 | 31 |
107.0 | 37 | ||||
237.0 | 25 | ||||
Mabuterol | 4.3 | 310.9 | 217.0 | 20 | 35 |
237.0 | 25 | ||||
Mapenterol | 4.5 | 324.9 | 217.0 | 146 | 33 |
Salbutamol | 3.2 | 239.9 | 148.0 | 131 | 25 |
166.1 | 19 | ||||
Ractopamine | 4.0 | 301.9 | 164.1 | 16 | 23 |
136.1 | 31 | ||||
Terbutaline | 3.2 | 225.9 | 152.1 | 121 | 23 |
125.1 | 35 | ||||
Zilpaterol | 3.1 | 261.9 | 185.0 | 26 | 35 |
202.0 | 27 | ||||
Clebuterol-D9 | 4.1 | 285.9 | 204.0 | 31 | 25 |
Isoxsuprine -D5 | 4.3 | 307.2 | 150.0 | 61 | 23 |
Mabuterol-D9 | 4.3 | 319.9 | 238.0 | 76 | 25 |
Mapenterol-D11 | 4.5 | 335.9 | 238.0 | 76 | 25 |
Ractopamine-D6 | 4.0 | 308.0 | 168.1 | 56 | 23 |
Salbutamol-D6 | 3.2 | 248.9 | 148.0 | 136 | 27 |
Matrix | Repeatability (RSDr,%) | Within-lab reproducibility (RSDwR,%) | CCα (μg kg−1) | CCβ (μg kg−1) | Apparent recovery (%) | Concentration range (ng/mL) | Matrix effect (%) | Expanded uncertainty (μg kg−1) |
---|---|---|---|---|---|---|---|---|
Clenbuterol | ||||||||
drinking water | 4.1 ± 3.8 | 4.5 ± 4.0 | 0.10 | 0.11 | 103.9 ± 4.3 | 0.1–1.6 | 4.3 ± 3.9% | 0.20 ± 0.02 |
feed | 6.2 ± 4.6 | 5.6 ± 6.1 | 2.50 | 2.70 | 104.5 ± 6.8 | 2.5–40.0 | 5.0 ± 3.9% | 5.00 ± 1.14 |
liver | 4.3 ± 4.1 | 5.1 ± 4.7 | 0.10 | 0.12 | 103.5 ± 6.2 | 0.1–1.6 | 6.1 ± 3.2% | 0.20 ± 0.06 |
urine | 4.6 ± 3.7 | 5.3 ± 4.4 | 0.10 | 0.11 | 102.6 ± 5.1 | 0.1–1.6 | 5.6 ± 2.7% | 0.20 ± 0.04 |
muscle | 5.9 ± 6.5 | 4.5 ± 7.6 | 0.05 | 0.06 | 109.1 ± 4.4 | 0.1–1.6 | 4.3 ± 6.5% | 0.10 ± 0.02 |
lung | 4.3 ± 3.8 | 4.8 ± 4.6 | 0.10 | 0.11 | 96.1 ± 4.9 | 0.1–1.6 | 5.3 ± 4.9% | 0.20 ± 0.05 |
Mabuterol | ||||||||
drinking water | 3.7 ± 3.6 | 4.7 ± 3.9 | 0.10 | 0.11 | 99.5 ± 4.8 | 0.1–1.6 | 4.0 ± 3.5% | 0.20 ± 0.03 |
feed | 5.6 ± 5.4 | 6.7 ± 5.6 | 2.50 | 2.62 | 106.2 ± 7.8 | 2.5–40.0 | 6.0 ± 3.3% | 5.00 ± 1.25 |
liver | 5.9 ± 5.8 | 7.3 ± 6.3 | 0.10 | 0.12 | 95.4 ± 7.3 | 0.1–1.6 | 6.3 ± 5.2% | 0.20 ± 0.05 |
urine | 5.2 ± 4.9 | 6.3 ± 5.1 | 0.10 | 0.11 | 103.0 ± 4.1 | 0.1–1.6 | 5.4 ± 4.9% | 0.20 ± 0.04 |
muscle | 6.3 ± 4.1 | 6.4 ± 5.0 | 0.05 | 0.06 | 95.9 ± 6.0 | 0.05–0.8 | 5.8 ± 5.0% | 0.10 ± 0.02 |
lung | 5.9 ± 3.9 | 6.6 ± 5.6 | 0.10 | 0.11 | 93.2 ± 4.6 | 0.1–1.6 | 6.3 ± 4.6% | 0.20 ± 0.04 |
Mapenterol | ||||||||
drinking water | 3.2 ± 3.4 | 4.1 ± 4.0 | 0.10 | 0.12 | 98.9 ± 4.8 | 0.1–1.6 | 4.0 ± 2.0% | 0.10 ± 0.02 |
feed | 5.4 ± 4.6 | 5.6 ± 7.1 | 2.50 | 2.61 | 105.1 ± 5.6 | 2.5–40.0 | 5.1 ± 2.9% | 5.00 ± 1.14 |
liver | 5.4 ± 5.0 | 6.4 ± 5.2 | 0.10 | 0.12 | 93.3 ± 5.6 | 0.1–1.6 | 5.4 ± 4.9% | 0.20 ± 0.04 |
urine | 5.3 ± 4.5 | 5.4 ± 4.9 | 0.20 | 0.12 | 102.0 ± 5.5 | 0.1–1.6 | 4.9 ± 5.1% | 0.20 ± 0.04 |
muscle | 4.1 ± 3.4 | 5.0 ± 4.2 | 0.05 | 0.06 | 97.0 ± 5.3 | 0.05–0.8 | 5.5 ± 4.1% | 0.10 ± 0.02 |
lung | 3.9 ± 3.7 | 5.4 ± 4.8 | 0.10 | 0.11 | 100.3 ± 6.1 | 0.1–1.6 | 4.8 ± 5.2% | 0.20 ± 0.05 |
Brombuterol | ||||||||
drinking water | 3.1 ± 3.3 | 4.2 ± 4.1 | 0.10 | 0.12 | 95.4 ± 4.3 | 0.1–1.6 | 4.0 ± 3.6% | 0.20 ± 0.05 |
feed | 5.6 ± 4.1 | 6.2 ± 4.0 | 2.50 | 2.67 | 103.2 ± 6.8 | 2.5–40.0 | 5.3 ± 3.9% | 5.00 ± 1.24 |
liver | 4.9 ± 4.7 | 5.3 ± 4.9 | 0.10 | 0.11 | 96.5 ± 6.8 | 0.1–1.6 | 6.1 ± 4.8% | 0.20 ± 0.05 |
urine | 4.5 ± 3.9 | 5.1 ± 4.1 | 0.10 | 0.11 | 99.6 ± 5.2 | 0.1–1.6 | 5.1 ± 4.6% | 0.20 ± 0.04 |
muscle | 4.6 ± 4.7 | 5.4 ± 4.8 | 0.05 | 0.06 | 105.4 ± 5.4 | 0.05–0.8 | 4.5 ± 5.3% | 0.10 ± 0.03 |
lung | 4.9 ± 3.5 | 5.4 ± 4.6 | 0.10 | 0.12 | 98.2 ± 4.9 | 0.1–1.6 | 5.3 ± 3.6% | 0.20 ± 0.05 |
Ractopamine | ||||||||
drinking water | 3.1 ± 3.7 | 4.6 ± 3.6 | 0.50 | 0.57 | 103.5 ± 3.9 | 0.5–8.0 | 3.9 ± 4.1% | 1.00 ± 0.23 |
feed | 4.4 ± 3.1 | 5.6 ± 4.6 | 2.50 | 2.56 | 102.3 ± 5.2 | 2.5–40.0 | 6.0 ± 3.2% | 5.00 ± 1.35 |
liver | 4.0 ± 4.3 | 4.6 ± 4.9 | 0.50 | 0.52 | 103.6 ± 6.8 | 0.5–8.0 | 5.3 ± 6.0% | 1.00 ± 0.26 |
urine | 4.2 ± 3.8 | 5.3 ± 4.2 | 0.50 | 0.52 | 102.8 ± 5.1 | 0.5–8.0 | 5.8 ± 5.3% | 1.00 ± 0.23 |
muscle | 4.6 ± 4.9 | 5.4 ± 5.3 | 0.50 | 0.52 | 105.0 ± 4.7 | 0.5–8.0 | 6.5 ± 5.7% | 1.00 ± 0.16 |
lung | 4.9 ± 4.5 | 5.4 ± 4.9 | 0.50 | 0.56 | 103.8 ± 4.3 | 0.5–8.0 | 5.5 ± 3.9% | 1.00 ± 0.26 |
Salbutamol | ||||||||
drinking water | 4.2 ± 2.4 | 5.4 ± 4.0 | 2.50 | 2.54 | 104.9 ± 4.8 | 2.5–40.0 | 5.3 ± 3.1% | 5.00 ± 1.25 |
feed | 5.9 ± 4.6 | 6.5 ± 6.6 | 2.50 | 2.72 | 105.1 ± 6.9 | 2.5–40.0 | 6.0 ± 4.1% | 5.00 ± 1.31 |
liver | 5.3 ± 4.7 | 7.4 ± 5.2 | 2.50 | 2.61 | 93.3 ± 5.6 | 2.5–40.0 | 6.5 ± 5.4% | 5.00 ± 1.45 |
urine | 4.9 ± 4.8 | 6.4 ± 4.9 | 0.50 | 0.52 | 97.0 ± 6.3 | 0.5–8.0 | 5.4 ± 5.5% | 1.00 ± 0.25 |
muscle | 5.2 ± 3.3 | 5.7 ± 4.3 | 2.50 | 2.61 | 105.3 ± 4.6 | 2.5–40.0 | 6.5 ± 6.5% | 5.00 ± 1.16 |
lung | 4.9 ± 4.5 | 5.0 ± 4.6 | 0.50 | 0.52 | 101.5 ± 5.1 | 0.5–8.0 | 4.8 ± 5.6% | 1.00 ± 0.33 |
Zilpaterol | ||||||||
drinking water | 3.2 ± 2.4 | 4.1 ± 3.0 | 2.50 | 2.58 | 105.4 ± 4.8 | 2.5–40.0 | 3.0 ± 2.1% | 5.00 ± 1.19 |
feed | 4.1 ± 4.5 | 5.9 ± 4.6 | 2.50 | 2.73 | 104.5 ± 6.3 | 2.5–40.0 | 5.3 ± 3.9% | 5.00 ± 1.40 |
liver | 6.4 ± 5.7 | 7.7 ± 6.2 | 2.50 | 2.79 | 107.3 ± 5.6 | 2.5–40.0 | 6.3 ± 6.3% | 5.00 ± 1.52 |
urine | 7.1 ± 4.3 | 8.0 ± 4.9 | 0.50 | 0.56 | 100.3 ± 6.8 | 0.5–8.0 | 5.3 ± 4.6% | 1.00 ± 0.23 |
muscle | 5.1 ± 4.3 | 5.9 ± 4.8 | 2.50 | 2.69 | 112.0 ± 6.1 | 2.5–40.0 | 7.3 ± 5.5% | 5.00 ± 1.64 |
lung | 4.0 ± 3.5 | 8.2 ± 7.6 | 0.50 | 0.56 | 106.4 ± 5.3 | 0.5–8.0 | 7.0 ± 4.3% | 1.00 ± 0.45 |
Terbutaline | ||||||||
drinking water | 5.2 ± 4.4 | 6.0 ± 4.5 | 5.00 | 5.34 | 106.9 ± 3.8 | 5.0–80.0 | 5.1 ± 4.6% | 10.00 ± 3.60 |
feed | 6.2 ± 3.6 | 7.6 ± 3.9 | 2.50 | 2.70 | 107.5 ± 7.1 | 2.5–40.0 | 6.6 ± 4.3% | 5.00 ± 1.72 |
liver | 4.2 ± 4.7 | 6.1 ± 5.7 | 5.00 | 5.25 | 93.3 ± 5.1 | 5.0–80.0 | 5.7 ± 6.1% | 10.00 ± 3.12 |
urine | 6.1 ± 4.2 | 7.3 ± 5.0 | 1.50 | 1.54 | 102.0 ± 6.5 | 1.5–24.0 | 8.8 ± 4.7% | 3.00 ± 0.86 |
muscle | 3.4 ± 3.2 | 4.2 ± 3.6 | 5.00 | 5.63 | 105.3 ± 3.8 | 5.0–80.0 | 3.5 ± 3.5% | 10.00 ± 4.1 |
lung | 5.2 ± 3.5 | 6.0 ± 4.0 | 1.50 | 1.64 | 96.3 ± 5.1 | 1.5–24.0 | 6.3 ± 6.2% | 3.00 ± 0.82 |
Isoxsuprine | ||||||||
drinking water | 3.6 ± 4.3 | 4.4 ± 5.1 | 0.25 | 0.27 | 102.9 ± 4.9 | 0.25–4.0 | 3.2 ± 2.3% | 0.50 ± 0.09 |
feed | 3.6 ± 3.7 | 4.6 ± 4.0 | 2.50 | 2.59 | 104.5 ± 2.8 | 2.5–40.0 | 5.0 ± 4.9% | 5.00 ± 1.19 |
liver | 3.8 ± 4.0 | 4.9 ± 5.2 | 0.25 | 0.26 | 102.3 ± 4.3 | 0.25–4.0 | 4.6 ± 4.5% | 0.50 ± 0.11 |
urine | 4.9 ± 3.8 | 5.4 ± 4.5 | 0.25 | 0.26 | 106.2 ± 4.6 | 0.25–4.0 | 5.3 ± 4.0% | 0.50 ± 0.12 |
muscle | 3.6 ± 3.8 | 4.3 ± 4.1 | 0.25 | 0.26 | 98.0 ± 4.3 | 0.25–4.0 | 4.5 ± 3.5% | 0.50 ± 0.08 |
lung | 3.6 ± 4.5 | 5.8 ± 5.6 | 0.25 | 0.27 | 108.0 ± 6.2 | 0.25–4.0 | 4.3 ± 5.0% | 0.50 ± 0.12 |
Analyte | drinking water μg kg−1 | feed μg kg−1 | liver μg kg−1 | urine μg kg−1 | muscle μg kg−1 | lung μg kg−1 |
---|---|---|---|---|---|---|
Brombuterol | 0.1; 0.2; 0.4; 1.6 | 2.5; 5.0; 10.0; 40.0 | 0.1; 0.2; 0.4; 1.6 | 0.1; 0.2; 0.4; 1.6 | 0.05; 0.1; 0.2; 0.8 | 0.1; 0.2; 0.4; 1.6 |
Clenbuterol | 0.1; 0.2; 0.4; 1.6 | 2.5; 5.0; 10.0; 40.0 | 0.1; 0.2; 0.4; 1.6 | 0.1; 0.2; 0.4; 1.6 | 0.05; 0.1; 0.2; 0.8 | 0.1; 0.2; 0.4; 1.6 |
Isoxsuprine | 0.25; 0.5; 1.0; 4.0 | 2.5; 5.0; 10.0; 40.0 | 0.25; 0.5; 1.0; 4.0 | 0.25; 0.5; 1.0; 4.0 | 0.25; 0.5; 1.0; 4.0 | 0.25; 0.5; 1.0; 4.0 |
Mabuterol | 0.1; 0.2; 0.4; 1.6 | 2.5; 5.0; 10.0; 40.0 | 0.1; 0.2; 0.4; 1.6 | 0.1; 0.2; 0.4; 1.6 | 0.05; 0.1; 0.2; 0.8 | 0.1; 0.2; 0.4; 1.6 |
Mapenterol | 0.1; 0.2; 0.4; 1.6 | 2.5; 5.0; 10.0; 40.0 | 0.1; 0.2; 0.4; 1.6 | 0.1; 0.2; 0.4; 1.6 | 0.05; 0.1; 0.2; 0.8 | 0.1; 0.2; 0.4; 1.6 |
Salbutamol | 2.5; 5.0; 10.0; 40.0 | 2.5; 5.0; 10.0; 40.0 | 2.5; 5.0; 10.0; 40.0 | 0.5; 1.0; 2.0; 8.0 | 2.5; 5.0; 10.0; 40.0 | 0.5; 1.0; 2.0; 8.0 |
Ractopamine | 0.5; 1.0; 2.0; 8.0 | 2.5; 5.0; 10.0; 40.0 | 0.5; 1.0; 2.0; 8.0 | 0.5; 1.0; 2.0; 8.0 | 0.5; 1.0; 2.0; 8.0 | 0.5; 1.0; 2.0; 8.0 |
Terbutaline | 5.0; 10.0; 20.0; 80.0 | 2.5; 5.0; 10.0; 40.0 | 5.0; 10.0; 20.0; 80.0 | 1.5; 3.0; 6.0; 24.0 | 5.0; 10.0; 20.0; 80.0 | 1.5; 3.0; 6.0; 24.0 |
Zilpaterol | 2.5; 5.0; 10.0; 40.0 | 2.5; 5.0; 10.0; 40.0 | 2.5; 5.0; 10.0; 40.0 | 0.5; 1.0; 2.0; 8.0 | 2.5; 5.0; 10.0; 40.0 | 0.5; 1.0; 2.0; 8.0 |