Prevalence of Toxoplasma gondii in retail fresh meat products from free-range chickens in Spain
, , et
06 oct. 2021
À propos de cet article
Catégorie d'article: Review article
Publié en ligne: 06 oct. 2021
Pages: 457 - 461
Reçu: 14 mars 2021
Accepté: 15 sept. 2021
DOI: https://doi.org/10.2478/jvetres-2021-0052
Mots clés
© 2021 M.J. Gracia Salinas et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1
Real-time positive PCR results for the chicken thigh samples used in this study
| Positive | Average | Estimated number | SAG1 PCR |
|---|---|---|---|
| samples | Ct | of copies |
product** |
| 1 | 35.30 | 2.1/g | positive |
| 4 | 34.60 | 3.4/g | positive |
| 14 | 36.16 | 1.1/g | positive |
| 15 | 34.31 | 4.1/g | positive |
| 20 | 35.71 | 1.6/g | positive |
Primers utilised for the detection of T_ gondii in this study
| Primer | Primer sequences | Gene | Length (nt) | Reference |
|---|---|---|---|---|
| ToxoRoc F | 5ʹ-TAGACGAGACGACGCTTTCC-3ʹ | 64 | ( |
|
| ToxoRoc R | 5ʹ- TCGCCCTCTTCTCCACTCT-3ʹ | sequence | ||
| ToxoRepeat 500 F | 5ʹ-CGCTGCAGGGAGGAAGACGAAAGTTG-3ʹ | |||
| ToxoRepeat 500 R | 5ʹ-CGCTGCAGACACAGTGCATCTGGATT-3ʹ | sequence | 529 | ( |
| ToxoSG1 F | 5ʹ-TCATCGGTCGTCAATAA-3ʹ | |||
| ToxoSG1 R | 5ʹ- CTTTGACTCCATCTTTCC-3ʹ | SAG1 | 123 | ( |