<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2020-0070_s_005_w2aab3b7c38b1b6b1aab1c15b1Aa"><h3>Introduction</h3><p>To date, there have been no panoramic studies of the serum metabolome in feline mammary carcinoma. As the first such study, metabolomics techniques were used to analyse the serum of cats with these tumours. Three important metabolic pathways of screened differential metabolites closely related to feline mammary carcinomas were analysed to lay a theoretical basis for further study of the pathogenesis of these carcinomas.</p></sec><sec id="j_jvetres-2020-0070_s_006_w2aab3b7c38b1b6b1aab1c15b2Aa"><h3>Material and Methods</h3><p>Blood in a 5–8 mL volume was sampled from twelve cats of the same breed and similar age (close to nine years on average). Six were feline mammary carcinoma patients and six were healthy. L glutamate, L alanine, succinate, adenine, hypoxanthine, and inosine were screened as were alanine, aspartate, and glutamate metabolism, the tricarboxylid acid (TCA) cycle, and purine metabolism. Data were acquired with LC-MS non-target metabolomics, multiple reaction monitoring target metabolomics, and multivariate statistical and bioinformatic analysis.</p></sec><sec id="j_jvetres-2020-0070_s_007_w2aab3b7c38b1b6b1aab1c15b3Aa"><h3>Results</h3><p>Expression of five of the metabolites was upregulated and only inosine expression was downregulated. Up- and downregulation of metabolites related to glycometabolism, potentiation of the TCA cycle, greater content of lipid mobilisation metabolites, and abnormality of amino acid metabolism were closely related to the occurrence of the carcinomas.</p></sec><sec id="j_jvetres-2020-0070_s_008_w2aab3b7c38b1b6b1aab1c15b4Aa"><h3>Conclusion</h3><p>These findings provide a new direction for further study of the mechanisms associated with cat mammary neoplasms.</p></sec></abstract>

IntroductionTo date, there have been no panoramic studies of the serum metabolome in feline mammary carcinoma. As the first such study, metabolomics techniques were used to analyse the serum of cats with these tumours. Three important metabolic pathways of screened differential metabolites closely related to feline mammary carcinomas were analysed to lay a theoretical basis for further study of the pathogenesis of these carcinomas.Material and MethodsBlood in a 5–8 mL volume was sampled from twelve cats of the same breed and similar age (close to nine years on average). Six were feline mammary carcinoma patients and six were healthy. L glutamate, L alanine, succinate, adenine, hypoxanthine, and inosine were screened as were alanine, aspartate, and glutamate metabolism, the tricarboxylid acid (TCA) cycle, and purine metabolism. Data were acquired with LC-MS non-target metabolomics, multiple reaction monitoring target metabolomics, and multivariate statistical and bioinformatic analysis.ResultsExpression of five of the metabolites was upregulated and only inosine expression was downregulated. Up- and downregulation of metabolites related to glycometabolism, potentiation of the TCA cycle, greater content of lipid mobilisation metabolites, and abnormality of amino acid metabolism were closely related to the occurrence of the carcinomas.ConclusionThese findings provide a new direction for further study of the mechanisms associated with cat mammary neoplasms.

Serum metabolomic analysis of feline mammary carcinomas based on LC-MS and MRM techniques

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

IntroductionTo date, there have been no panoramic studies of the serum metabolome in feline mammary carcinoma. As the first such study, metabolomics techniques...

Serum metabolomic analysis of feline mammary carcinomas based on LC-MS and MRM techniques

Publié en ligne: 06 nov. 2020

Pages: 581 - 588

Reçu: 16 avr. 2020

Accepté: 07 oct. 2020

DOI: https://doi.org/10.2478/jvetres-2020-0070

Mots clés
cats, feline mammary carcinomas, LC-MS, metabolomics, multiple reaction monitoring (MRM)

© 2020 J. Zheng et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Serum metabolomic analysis of feline mammary carcinomas based on LC-MS and MRM techniques

Publié en ligne: 06 nov. 2020

Pages: 581 - 588

Reçu: 16 avr. 2020

Accepté: 07 oct. 2020

DOI: https://doi.org/10.2478/jvetres-2020-0070

Mots cléscats, feline mammary carcinomas, LC-MS, metabolomics, multiple reaction monitoring (MRM)

© 2020 J. Zheng et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
cats, feline mammary carcinomas, LC-MS, metabolomics, multiple reaction monitoring (MRM)