Detection of myxoma virus in the classical form of myxomatosis using an AGID assay: statistical assessment of the assay’s diagnostic performance
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14 juil. 2020
À propos de cet article
Catégorie d'article: Short Communication
Publié en ligne: 14 juil. 2020
Pages: 369 - 372
Reçu: 03 janv. 2020
Accepté: 01 juil. 2020
DOI: https://doi.org/10.2478/jvetres-2020-0049
Mots clés
© 2020 E. Kwit et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1
Results of samples tested using AGID and molecular methods with confidence intervals (CI) determined by the Clopper–Pearson method
| Molecular methods/ type of samples | Number of tested and PCR-positive/negative samples | AGID-confirmed results | Assessed results Median values obtained from proportions of confirmed results in AGID to the total number of samples for which test results agreed positively and negatively |
References |
|---|---|---|---|---|
| IAC-PCR (3,125 PCRU DNA of MYXV/sample) | 20 | 20 | 1.00 (0.83–1.00) | |
| IAC-PCR (1 PCRU DNA of MYXV/sample) | 20 | 0 | 0.00 (0.00–0.17) | |
| IAC-PCR (rabbit skin homogenates) | 23 | 18 | 0.78 (0.56–0.93) | This study |
| OIE PCR and OIE real-time PCR (control reference material) | 8 | 8 | 1.00 (0.63–1.00) | |
| OIE PCR and OIE real-time PCR (control reference material without MYXV) | 4 | 4 | 1.00 (0.40–1.00) | |
| All positive (with MYXV) | 71 | 46 | 0.65 (0.53–0.76) | |
| All samples in this study (accuracy) | 75 | 50 | 0.67 (0.55–0.76) |