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Metagenomic analysis of acquired antibiotic resistance determinants in the gut microbiota of wild boars (Sus scrofa) – preliminary results

Balázs Libisch
Balázs Libisch
, 
Tibor Keresztény
Tibor Keresztény
, 
Zoltán Kerényi
Zoltán Kerényi
, 
Róbert Kocsis
Róbert Kocsis
, 
Rita Sipos
Rita Sipos
, 
Péter P. Papp
Péter P. Papp
 et 
Ferenc Olasz
Ferenc Olasz
   | 29 févr. 2020
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Publié en ligne: 29 févr. 2020
Pages: 111 - 118
Reçu: 03 juin 2019
Accepté: 13 févr. 2020
DOI: https://doi.org/10.2478/jvetres-2020-0015
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© 2020 B. Libisch et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Phylum-level bacterial composition of the microbiota in three sections of the gastrointestinal tract of the diarrhoeic wild boar (WB) from Hungary analysed in this work in comparison with those established earlier for the domestic pig (DP) by a meta-analysis (13)
Phylum-level bacterial composition of the microbiota in three sections of the gastrointestinal tract of the diarrhoeic wild boar (WB) from Hungary analysed in this work in comparison with those established earlier for the domestic pig (DP) by a meta-analysis (13)

Fig. 2

GrapeTree diagram generated in EnteroBase based on seven-gene MLST profiles of 104 E. coli strains isolated in Europe from wild boars and domestic pigs (2, 28, 29). Countries of origin (and number of strains): Denmark (48), Hungary (8), Czech Republic (7), Spain (7), Ireland (6), Luxembourg (5), Italy (4), Switzerland (3), United Kingdom (3), Croatia (2), France (2), Germany (2), Portugal (2), Austria (1), Belgium (1), Bulgaria (1), Netherlands (1), and Sweden (1). Dark blue, light blue, and orange indicate E. coli isolates of domestic pig, wild boar, and food origin, respectively. Numbers stand for sequence types by the Achtman seven-gene MLST scheme (28). The node size in the GrapeTree diagram is proportional to the number of bacterial strains with the same sequence type (ST), and the length of edges are proportional to inter-node genetic distances (29). ST388 determined for the E. coli O112ab:H2 strain characterised in this work is marked by an asterisk (*)
GrapeTree diagram generated in EnteroBase based on seven-gene MLST profiles of 104 E. coli strains isolated in Europe from wild boars and domestic pigs (2, 28, 29). Countries of origin (and number of strains): Denmark (48), Hungary (8), Czech Republic (7), Spain (7), Ireland (6), Luxembourg (5), Italy (4), Switzerland (3), United Kingdom (3), Croatia (2), France (2), Germany (2), Portugal (2), Austria (1), Belgium (1), Bulgaria (1), Netherlands (1), and Sweden (1). Dark blue, light blue, and orange indicate E. coli isolates of domestic pig, wild boar, and food origin, respectively. Numbers stand for sequence types by the Achtman seven-gene MLST scheme (28). The node size in the GrapeTree diagram is proportional to the number of bacterial strains with the same sequence type (ST), and the length of edges are proportional to inter-node genetic distances (29). ST388 determined for the E. coli O112ab:H2 strain characterised in this work is marked by an asterisk (*)

Fig. 3

Immediate genetic environment of the tetracycline resistance determinants identified from the gut microbiome of free-living wild boars in Hungary. Schematic diagrams of metagenomic contigs for the tetQ (A), tetW (B), and tetO genes (C), respectively. Abbreviations for selected genes: traG – conjugal transfer protein TraG; tnp – transposase; mobC – mobilisation protein MobC; tnpV – transposon-encoded protein TnpV; and ctp – conjugal transfer protein
Immediate genetic environment of the tetracycline resistance determinants identified from the gut microbiome of free-living wild boars in Hungary. Schematic diagrams of metagenomic contigs for the tetQ (A), tetW (B), and tetO genes (C), respectively. Abbreviations for selected genes: traG – conjugal transfer protein TraG; tnp – transposase; mobC – mobilisation protein MobC; tnpV – transposon-encoded protein TnpV; and ctp – conjugal transfer protein

Fig. 4

Proportions of the sales of various veterinary antimicrobial agents in three countries of the European Union in 2015 (9) compared to those in Japan in 2013 for food producing animals (4)
Proportions of the sales of various veterinary antimicrobial agents in three countries of the European Union in 2015 (9) compared to those in Japan in 2013 for food producing animals (4)

Examples of acquired resistance determinants against various antibiotic classes in wastewater metagenomes in Hungary

Location Tetracyclines Macrolides Sulphonamides β-lactams Aminoglycosides NCBI SRA Codes
North Pest tetW, tetO, tetM mefA, mphE, ermB, ermF, ermG sul1 blaOXA aadA11, aadA1, aph(6)-Id ERR2607463
North Pest tetQ, tetW, tetO, tetM mefA, mphE, ermB, ermF, ermG sul1, sul2 cfxA , blaOXA, blaTEM aadA11, aph(6)-Id ERR1713361
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Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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