<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold><italic>Coxiella</italic> (<italic>C</italic>.) <italic>burnetii</italic>, the aetiological agent of Q fever, is able to modulate the macrophage/T-lymphocyte axis in an infected organism and impair synthesis of monokines and lymphokines.<bold>Material and Methods:</bold> The purpose of this research was to determine the levels of the cytokines that play a key role in the response to <italic>C. burnetii</italic> antigens (IL-1β, IL-2, IL-6, IL-10, IFN-γ and TNF-α) in the serum of animals originating from an infected herd prior to vaccination (day 0) and at 1, 7, and 21 days afterwards.<bold>Results:</bold> The vaccination of animals did not affect the production of IL-6, IL-1β, or IL-2. The serum levels of these cytokines were too low to measure in most of the samples. The initial levels of TNFα, IFNγ, and IL-10 were higher in seropositive than in seronegative animals, although significant differences between seropositive shedders and seropositive nonshedders appeared only in the levels of IFNγ and IL-10. Additionally, the course of the post-vaccination response concerning these two cytokines was different among seronegative nonshedders, seropositive nonshedders, and seropositive shedders.<bold>Conclusion:</bold> It seems that analysis of the IFNγ and IL-10 concentrations in animal blood serum may have some practical value in an assessment of the health status of seropositive animals and post-vaccination response.</abstract>

Introduction:Coxiella (C.) burnetii, the aetiological agent of Q fever, is able to modulate the macrophage/T-lymphocyte axis in an infected organism and impair synthesis of monokines and lymphokines.Material and Methods: The purpose of this research was to determine the levels of the cytokines that play a key role in the response to C. burnetii antigens (IL-1β, IL-2, IL-6, IL-10, IFN-γ and TNF-α) in the serum of animals originating from an infected herd prior to vaccination (day 0) and at 1, 7, and 21 days afterwards.Results: The vaccination of animals did not affect the production of IL-6, IL-1β, or IL-2. The serum levels of these cytokines were too low to measure in most of the samples. The initial levels of TNFα, IFNγ, and IL-10 were higher in seropositive than in seronegative animals, although significant differences between seropositive shedders and seropositive nonshedders appeared only in the levels of IFNγ and IL-10. Additionally, the course of the post-vaccination response concerning these two cytokines was different among seronegative nonshedders, seropositive nonshedders, and seropositive shedders.Conclusion: It seems that analysis of the IFNγ and IL-10 concentrations in animal blood serum may have some practical value in an assessment of the health status of seropositive animals and post-vaccination response.

Early cytokine response after vaccination with <italic>Coxiella burnetii</italic> phase I in an infected herd of dairy cattle

Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine

University of Warmia and Mazury in Olsztyn

Department of Cattle and Sheep Diseases/Laboratory of Serological Diagnosis, National Veterinary Research Institute

Department of Internal Diseases with Clinic, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Early cytokine response after vaccination with  phase I in an infected herd of dairy cattle

Introduction:Coxiella (C.) burnetii, the aetiological agent of Q fever, is able to modulate the macrophage/T-lymphocyte axis in an infected organism and impair...

Early cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattle

Publié en ligne: 31 déc. 2018

Pages: 469 - 476

Reçu: 06 sept. 2018

Accepté: 11 déc. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0076

Mots clés
dairy cattle, vaccine, cytokines

© 2018 Joanna Małaczewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Early cytokine response after vaccination with Coxiella burnetii phase I in an infected herd of dairy cattle

Publié en ligne: 31 déc. 2018

Pages: 469 - 476

Reçu: 06 sept. 2018

Accepté: 11 déc. 2018

DOI: https://doi.org/10.2478/jvetres-2018-0076

Mots clésdairy cattle, vaccine, cytokines

© 2018 Joanna Małaczewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
dairy cattle, vaccine, cytokines