<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences, flowers, cotyledons, hypocotyls/epicotyls, and embryos). After almost 45 years of research, this culture system has been used for several monocotyledonous and dicotyledonous plants of commercial importance, and for model plants. The limited amount of cells in a TCL is of paramount importance because marker molecules/genes of differentiation can be easily localized <italic>in situ</italic> in the target/responsive cells. Thus, the use of TCLs has allowed, and continues to allow, for the expansion of knowledge in plant research in a practical and applied manner into the fields of tissue culture and micropropagation, cell and organ genetics, molecular biology, biochemistry, and development. Starting from a brief historical background, the actual and potential uses of the TCL system are briefly reviewed.</p></abstract>

Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences, flowers, cotyledons, hypocotyls/epicotyls, and embryos). After almost 45 years of research, this culture system has been used for several monocotyledonous and dicotyledonous plants of commercial importance, and for model plants. The limited amount of cells in a TCL is of paramount importance because marker molecules/genes of differentiation can be easily localized in situ in the target/responsive cells. Thus, the use of TCLs has allowed, and continues to allow, for the expansion of knowledge in plant research in a practical and applied manner into the fields of tissue culture and micropropagation, cell and organ genetics, molecular biology, biochemistry, and development. Starting from a brief historical background, the actual and potential uses of the TCL system are briefly reviewed.

The Untapped Potential Of Plant Thin Cell Layers

Sapienza – Università di Roma, Rome, Italy

Research Institute of Nyíregyháza, University of Debrecen, Hungary

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"The Untapped Potential Of Plant Thin Cell Layers"}

Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences,...

The Untapped Potential Of Plant Thin Cell Layers

Publié en ligne: 30 déc. 2015

Pages: 127 - 131

Reçu: 01 sept. 2015

Accepté: 01 nov. 2015

DOI: https://doi.org/10.2478/johr-2015-0024

Mots clés
model plants, organogenesis, plant biotechnology, TCLs

© 2015 Jaime Teixeira da Silva et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The Untapped Potential Of Plant Thin Cell Layers

Publié en ligne: 30 déc. 2015

Pages: 127 - 131

Reçu: 01 sept. 2015

Accepté: 01 nov. 2015

DOI: https://doi.org/10.2478/johr-2015-0024

Mots clésmodel plants, organogenesis, plant biotechnology, TCLs

© 2015 Jaime Teixeira da Silva et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
model plants, organogenesis, plant biotechnology, TCLs