<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, <italic>Hoplolaimus seinhorsti</italic> and <italic>H. pararobustus</italic>, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC and bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 of 28S rRNA, 1 ITS rRNA and 7 <italic>COI</italic> mtDNA (the first <italic>COI</italic> sequences of <italic>H. seinhorsti</italic> and <italic>H. pararobustus</italic>), and an updated morphological character comparison of 37 <italic>Hoplolaimus</italic> species is presented. The tree-based molecular species-delimitation approaches employed gave markedly differing results, and also showed remarkable discrepancies among the investigated genes, although the bPTP output was found to agree well with established morphological species delimitations. Both species-delimitation approaches did, however, provide the same output for the <italic>COI</italic> mtDNA sequences, and the <italic>COI</italic> mtDNA gene sequence was also found to correspond better to established morphological species. It is therefore recommended by this paper as representing the most suitable barcode marker for <italic>Hoplolaimus</italic> species identification. This integrative study also resulted in the corrective reassignment of 17 gene sequences that were previously unidentified or incorrectly classified, as well as concluding that <italic>H. pararobustus</italic> consists of two cryptic species.</p>
</abstract>

The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H. pararobustus, are provided based on an integrative study that includes light and scanning electron microscopy, phylogenetic analysis and two tree-based molecular species delimitation methods (GMYC and bPTP). Nineteen new sequences were obtained, including 5 partial 18S rRNA, 6 D2-D3 of 28S rRNA, 1 ITS rRNA and 7 COI mtDNA (the first COI sequences of H. seinhorsti and H. pararobustus), and an updated morphological character comparison of 37 Hoplolaimus species is presented. The tree-based molecular species-delimitation approaches employed gave markedly differing results, and also showed remarkable discrepancies among the investigated genes, although the bPTP output was found to agree well with established morphological species delimitations. Both species-delimitation approaches did, however, provide the same output for the COI mtDNA sequences, and the COI mtDNA gene sequence was also found to correspond better to established morphological species. It is therefore recommended by this paper as representing the most suitable barcode marker for Hoplolaimus species identification. This integrative study also resulted in the corrective reassignment of 17 gene sequences that were previously unidentified or incorrectly classified, as well as concluding that H. pararobustus consists of two cryptic species.

Characterization of <italic>Hoplolaimus seinhorsti</italic> and <italic>Hoplolaimus pararobustus</italic> (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus <italic>Hoplolaimus</italic>

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Characterization of  and  (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus 

The morphological and molecular characterisations of two lance nematode species isolated from the rhizosphere of banana, Hoplolaimus seinhorsti and H....

Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus

Article Category: Research Paper

Publié en ligne: 23 mai 2023

Pages: -

Reçu: 19 nov. 2022

DOI: https://doi.org/10.2478/jofnem-2023-0019

Mots clés
28S rDNA, ITS rDNA, Banana, Lance nematode, Morphology, Phylogeny, Plant-parasitic nematode, Scanning electron microscopy, Systematic, Taxonomy

© 2023 Emmanuel Olajide et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina: Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus

Article Category: Research Paper

Publié en ligne: 23 mai 2023

Pages: -

Reçu: 19 nov. 2022

DOI: https://doi.org/10.2478/jofnem-2023-0019

Mots clés28S rDNA, ITS rDNA, Banana, Lance nematode, Morphology, Phylogeny, Plant-parasitic nematode, Scanning electron microscopy, Systematic, Taxonomy

© 2023 Emmanuel Olajide et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Mots clés
28S rDNA, ITS rDNA, Banana, Lance nematode, Morphology, Phylogeny, Plant-parasitic nematode, Scanning electron microscopy, Systematic, Taxonomy