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Complete Mitogenome of Cruznema tripartitum Confirms Highly Conserved Gene Arrangement within Family Rhabditidae

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Figure 1

Morphology and mitogenome structure of Cruznema tripartitum. (A, B): Head region; (C): Anterior part of body; (D): Female tail; (E): Lateral view of male tail; (F): Ventral view of male tail; (G): Mitogenome structure. The genes are denoted by the color block and encoded in the clockwise direction. The leucine and serine tRNA genes are separated by their anticodon sequences: L1 is trnL-cta, L2 is trnL-tta, S1 is trnS-aga, and S2 is trnS-tca. The region without color indicates the non-coding region.
Morphology and mitogenome structure of Cruznema tripartitum. (A, B): Head region; (C): Anterior part of body; (D): Female tail; (E): Lateral view of male tail; (F): Ventral view of male tail; (G): Mitogenome structure. The genes are denoted by the color block and encoded in the clockwise direction. The leucine and serine tRNA genes are separated by their anticodon sequences: L1 is trnL-cta, L2 is trnL-tta, S1 is trnS-aga, and S2 is trnS-tca. The region without color indicates the non-coding region.

Figure 2

Predicted secondary structure of tRNA in the mitochondrial genome of Cruznema tripartitum. tRNA, transfer RNA.
Predicted secondary structure of tRNA in the mitochondrial genome of Cruznema tripartitum. tRNA, transfer RNA.

Figure 3

Maximum likelihood phylogenetic tree inferred from amino acid sequences of 12 concatenated protein-coding genes containing 3,893 alignment characters. The newly obtained sequence is indicated with asterisk.
Maximum likelihood phylogenetic tree inferred from amino acid sequences of 12 concatenated protein-coding genes containing 3,893 alignment characters. The newly obtained sequence is indicated with asterisk.

Figure 4

Linearized mitochondrial gene arrangement patterns of Cruznema tripartitum (indicated in bold) and other related species in suborders Rhabditina and Tylenchina. All genes are encoded in the same direction. All 22 tRNA genes are designated by a single letter amino acid code, based on the International Union of Pure and Applied Chemistry code. The two leucine and two serine tRNA genes are labeled according to their distinct anticodon sequences as L1 (trnL1-cta), L2 (trnL2-tta), S1 (trnS1-aga), and S2 (trnS2-tca). The non-coding regions are not included.
Linearized mitochondrial gene arrangement patterns of Cruznema tripartitum (indicated in bold) and other related species in suborders Rhabditina and Tylenchina. All genes are encoded in the same direction. All 22 tRNA genes are designated by a single letter amino acid code, based on the International Union of Pure and Applied Chemistry code. The two leucine and two serine tRNA genes are labeled according to their distinct anticodon sequences as L1 (trnL1-cta), L2 (trnL2-tta), S1 (trnS1-aga), and S2 (trnS2-tca). The non-coding regions are not included.

Mitochondrial genome organization in Cruznema tripartitum.

Gene name Start Stop Size (bp) Intergenic space Codons
COX1 1 1575 1,575 0 ATT/TAA
trnC (tgc) 1587 1640 54 11
trnM (atg) 1643 1702 60 2
trnD (gac) 1703 1757 55 0
trnG (gga) 1758 1812 55 0
COX2 1813 2508 696 0 ATT/TAA
trnH (cac) 2512 2568 57 3
rrnL 2569 3525 957 0
ND3 3526 3861 336 0 ATT/TAA
ND5 3862 5445 1,584 0 ATT/TAA
trnA (gca) 5448 5502 55 2
trnP (cca) 6199 6252 54 696
trnV (gta) 6253 6306 54 0
ND6 6307 6741 435 0 ATT/TAA
ND4L 6742 6975 234 0 ATT/TAA
trnW (tga) 6975 7031 57 –1
trnE (gaa) 7037 7092 56 5
rrnS 7093 7789 697 0
trnS2 (tca) 7788 7841 54 –2
trnN (aac) 7842 7897 56 0
trnY (tac) 7901 7957 57 3
ND1 7955 8830 876 –3 TTG/TAA
ATP6 8860 9429 570 29 ATA/TAA
trnK (aaa) 9438 9499 62 8
trnL2 (tta) 9508 9563 56 8
trnS1 (aga) 9564 9619 56 0
ND2 9620 10465 846 0 ATT/TAA
trnI (atc) 10466 10526 61 0
trnR (cgt) 10528 10583 56 1
trnQ (caa) 10584 10638 55 0
trnF (ttc) 10647 10703 57 8
COB 10704 11816 1,113 0 ATA/TAA
trnL1 (cta) 11821 11875 55 4
COX3 11876 12643 768 0 ATT/TAA
trnT (aca) 12654 12708 55 10
ND4 12709 13941 1,233 126 TTG/TAA
eISSN:
2640-396X
Langue:
Anglais
Périodicité:
Volume Open
Sujets de la revue:
Life Sciences, other