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Figure 1
Interaction between Fol 3 and cultivar on egg production of Tasti Lee isolines (with and without I-3 and Mi genes) at 28 DAT in experiment 1. TL, Tasti Lee; +, with, -, without; DAT, days after transplanting.
Note: Although effects are grouped by genetic background, letters indicating significance apply across all means.
Figure 2
Interaction between Fol race 3, RKN (M. javanica), and Tasti Lee isolines on plant growth of all four Tasti Lee isolines (with and without I-3 and Mi genes) at 28 DAT in experiment 1. TL, Tasti Lee; +, with, -, without; DAT, days after transplanting.
Note: Although effects are grouped by genetic background, letters indicating significance apply across all means.
Figure 3
Effect of Fol (A) and RKN (B) on plant height by time in experiment 1. Fus, Fusarium wilt; Nem, root-knot nematode.
Figure 4
Interaction between Fol race 3, RKN (M. javanica), and Tasti Lee isolines on shoot biomass of all four Tasti Lee isolines (with and without I-3 and Mi genes) at 30 DAT in experiment 1. TL, Tasti Lee; +, with, -, without; DAT, days after transplanting. Note: Although effects are grouped by genetic background, letters indicating significance apply across all means.
Figure 5
Interaction between Fol race 3, RKN (M. javanica), and Tasti Lee isolines on root biomass of all four Tasti Lee isolines (with and without I-3 and Mi genes) at 30 DAT in experiment 1. TL, Tasti Lee; +, with, -, without; DAT, days after transplanting. Note: Although effects are grouped by genetic background, letters indicating significance apply across all means.
Figure 6
Interaction between Fol and cultivar on egg production of two Tasti Lee isolines (with and without Mi gene) at 42 DAT in experiment 2. TL, Tasti Lee; +, with, -, without; DAT, days after transplanting; 0 DAT, inoculated at planting; 10 DAT, inoculated 10 days after transplanting. Note: Although effects are grouped by genetic background, letters indicating significance apply across all means.
Figure 7
Effect of Fol on J2 penetration at 10 DAT on Tasti Lee isoline without Mi gene in experiment 2. J2, second stage RKN juvenile; +, with, −, without; DAT, days after transplanting; 0 DAT, inoculated at planting; 10 DAT, inoculated 10 days after transplanting.
Figure 8
Interaction between Fol race 3 and RKN (M. javanica) on plant growth of two Tasti Lee isolines (without I-3 gene and with or without Mi gene) at 28 and 42 DAT in experiment 2. DAT, days after transplanting; 0 DAT, inoculated at planting; 10 DAT, inoculated 10 days after transplanting.
Figure 9
Effect of Fol (A) and RKN (B) on plant height by time in experiment 2. Fus, Fusarium wilt; Nem, root-knot nematode; 0D, 0 days after transplanting; 10D, 10; DAT, days after transplanting.
Figure 10
Interaction between Fol race 3 and RKN (M. javanica) on root and shoot biomass of two Tasti Lee isolines (without I-3 gene and with or without Mi gene) at 42 DAT in experiment 2. DAT, days after transplanting; 0 DAT, inoculated at planting; 10 DAT, inoculated 10 days after transplanting.
Figure 11
Effect of RKN and Fol and its combination on plant growth and development in tomato. (A) Tasti Lee isolines with Mi gene and (B) Tasti Lee isoline without Mi gene in experiment 2. In both pictures, five plants infected with Fol alone at right and five with Fol + M. javanica at left. Photo credit: Homan Regmi.
Effect of RKN (M. javanica) and Fol race 3 on plant height and biomass of Tasti Lee isolines with and without the Mi gene in experiment 2.
Factor
Level
Plant height (cm)
Dry weight (gm) at 42 DAT
0 DAT
14 DAT
28 DAT
42 DAT
Root
Shoot
RKN
Yes
6.8 a
13.0 b
35.7 b
47.1 b
1.3 b
6.8 b
None
6.6 a
14.7 a
45.1 a
61.4 a
1.9 a
9.7 a
Fol
0 DAT
6.5 b
12.9 b
35.3 b
50.2 b
1.2 b
6.5 c
10 DAT
7.0 a
14.7 a
44.5 a
58.4 a
1.6 a
7.9 b
None
6.6 b
13.9 ab
41.5 a
54.1 ab
1.9 a
10.4 a
Cultivar
TL (−I-3, −Mi)
6.7 a
14.2 a
39.0 b
52.1 a
1.5 a
8.0 a
TL (−I-3, +Mi)
6.7 a
13.5 a
41.8 a
56.4 a
1.7 a
8.5 a
P-value
RKN
0.479
0.0008
<0.0001
<0.0001
<0.0001
<0.0001
Fol
0.037
0.009
<0.0001
0.049
<0.0001
<0.0001
RKN*Fol
0.374
0.470
<0.0001
0.0001
0.008
0.0004
Cultivar
0.919
0.151
0.049
0.105
0.219
0.278
RKN*Cultivar
0.479
0.275
0.154
0.652
0.652
0.009
Fol *Cultivar
0.352
0.406
0.388
0.288
0.076
0.130
RKN*Fol*Cultivar
0.374
0.430
0.936
0.964
0.804
0.241
Effect of Fol race 3 on RKN (M. javanica) severity in Tasti Lee isolines with and without Mi and I-3 genes in experiment 1.
Factors
Level
*Gall index (30 DAT)
Eggs/root (30 DAT)
RF (30 DAT)
Fol
Yes
1.1 b
2,921 b
0.3 b
None
2.7 a
33,580 a
3.4 a
Cultivars
TL (+/-3, +Mi)
0.5 b
140 d
0.01 b
TL (−/-3, +Mi)
0.6 b
379 c
0.03 b
TL (+/-3, −Mi)
3.2 a
32,736 b
3.3 a
TL (−/-3, −Mi)
3.5 a
39,750 a
4.0 a
P-value
Fol
<0.0001
<0.0001
0.0004
Cultivar
<0.0001
<0.0001
0.0008
Fol*Cultivar
0.212
0.003
0.004
Comparisons of plant biomass in non-treated control with different combinations of RKN (M. javanica) and Fol race 3 in experiments 1 and 2.
Experiment 1
Comparison
Estimated difference and level of significance
Shoot
P-value
Root
P-value
NTC vs RKN
0.9
<0.0001
−0.1
0.036
NTC vs Fol
3.4
<0.0001
0.4
<0.0001
NTC vs RKN+Fol
4.0
<0.0001
0.7
<0.0001
Experiment 2
NTC vs RKN
1.2
0.120
0.3
0.106
NTC vs Fol (0 DAT)
1.7
0.024
0.4
0.1007
NTC vs RKN+Fol (0 DAT)
7.3
<0.0001
1.5
<0.0001
NTC vs Fol (10 DAT)
2.1
0.008
0.4
0.098
NTC vs RKN+Fol (10 DAT)
4.1
<0.0001
0.6
0.007
Effect of Fol race 3 on RKN (M. javanica) severity in Tasti Lee isolines with and without Mi gene in experiment 2.
Factors
Level
*Gall index (42 DAT)
Eggs/root (42 DAT)
RF (42 DAT)
Fol
0 DAT
4.5 a
44,101 b
4.4 b
10 DAT
5.1 a
149,912 a
15.0 a
None
5.2 a
162,088 a
16.2 a
Cultivars
TL (−I-3, −Mi)
5.6 a
161,328 a
16.1 b
TL (−I-3, +Mi)
4.3 b
76,072 b
7.6 a
P-value
Fol
0.278
0.0002
0.002
Cultivar
0.001
0.05
0.003
Fol*Cultivar
0.329
0.001
0.005
Effect of RKN (M. javanica) and Fol race 3 on plant height and biomass of Tasti Lee isolines with and without Mi and I-3 genes in experiment 1.
