<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Although liquid-liquid extraction methods are currently being applied in many areas such as analytical chemistry, biochemical engineering, biochemistry, and biological applications, accessibility and usability of microfluidics in practical daily life fields are still bounded. Suspended microfluidic devices have the potential to lessen the obstacles, but the absence of robust design rules have hampered their usage. The primary objective of this work is to design and fabricate a microfluidic device to quantitatively monitor the drug uptake of cancer cells. Liquid-liquid extraction is used to quantify the drug uptake. In this research work, designs and simulations of two different microfluidic devices for carrying out multiplex solution experiments are proposed to test their efficiency. These simplified miniaturized chips would serve as suspended microfluidic metabolites extraction platform as it allows extracting the metabolites produced from the cancer cells as a result of applying a specific drug type for a certain period of time. These devices would be fabricated by making polydimethylsiloxane (PDMS) molds from the negative master mold using soft lithography. Furthermore, it can leverage to provide versatile functionalities like high throughput screening, cancer cell invasions, protein purification, and small molecules extractions. As per previous studies, PDMS has been depicting better stability with various solvents and has proved to be a reliable and cost effective material to be used for fabrication, though the sensitivity of the chip would be analyzed by cross contamination and of solvents within the channels of device.</p></abstract>

Although liquid-liquid extraction methods are currently being applied in many areas such as analytical chemistry, biochemical engineering, biochemistry, and biological applications, accessibility and usability of microfluidics in practical daily life fields are still bounded. Suspended microfluidic devices have the potential to lessen the obstacles, but the absence of robust design rules have hampered their usage. The primary objective of this work is to design and fabricate a microfluidic device to quantitatively monitor the drug uptake of cancer cells. Liquid-liquid extraction is used to quantify the drug uptake. In this research work, designs and simulations of two different microfluidic devices for carrying out multiplex solution experiments are proposed to test their efficiency. These simplified miniaturized chips would serve as suspended microfluidic metabolites extraction platform as it allows extracting the metabolites produced from the cancer cells as a result of applying a specific drug type for a certain period of time. These devices would be fabricated by making polydimethylsiloxane (PDMS) molds from the negative master mold using soft lithography. Furthermore, it can leverage to provide versatile functionalities like high throughput screening, cancer cell invasions, protein purification, and small molecules extractions. As per previous studies, PDMS has been depicting better stability with various solvents and has proved to be a reliable and cost effective material to be used for fabrication, though the sensitivity of the chip would be analyzed by cross contamination and of solvents within the channels of device.

Design and simulation of microfluidic device for metabolite screening and quantitative monitoring of drug uptake in cancer cells

Journal of Electrical Bioimpedance

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Although liquid-liquid extraction methods are currently being applied in many areas such as analytical chemistry, biochemical engineering, biochemistry, and...

Design and simulation of microfluidic device for metabolite screening and quantitative monitoring of drug uptake in cancer cells

Article Category: Research articles

Publié en ligne: 16 août 2018

Pages: 10 - 16

Reçu: 05 févr. 2018

DOI: https://doi.org/10.2478/joeb-2018-0003

Mots clés
Microfluidics, micro and nano fabrication, photolithography, drug uptake, cancer cells, multiphysics simulation, in vitro, metabolite screening

© 2018 Asif A, Khawaldeh S, Khan MS, Tekin A, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Design and simulation of microfluidic device for metabolite screening and quantitative monitoring of drug uptake in cancer cells

Article Category: Research articles

Publié en ligne: 16 août 2018

Pages: 10 - 16

Reçu: 05 févr. 2018

DOI: https://doi.org/10.2478/joeb-2018-0003

Mots clésMicrofluidics, micro and nano fabrication, photolithography, drug uptake, cancer cells, multiphysics simulation, in vitro, metabolite screening

© 2018 Asif A, Khawaldeh S, Khan MS, Tekin A, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Mots clés
Microfluidics, micro and nano fabrication, photolithography, drug uptake, cancer cells, multiphysics simulation, in vitro, metabolite screening